{"title":"{Disable Category} Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability","description":"\u003cp\u003e{Disable Category} Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability — reagen TCI untuk riset dan industri. Tersedia di Indonesia melalui AMI Scientific.\u003c\/p\u003e","products":[{"product_id":"tci2510e037028116","title":"TCI E0370 1239-45-8 Ethidium Bromide","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eTCI E0370 Ethidium Bromide is a nucleic acid intercalating dye that has served for decades as a dependable reagent for the visualization of DNA and RNA in molecular biology laboratories. The molecule inserts itself between the base pairs of double-stranded nucleic acids, and once bound, its fluorescence intensity increases sharply under ultraviolet illumination. Its practical role is straightforward: after samples have been resolved by electrophoresis on an agarose gel, this dye converts DNA bands that would otherwise remain invisible into glowing bands that can be photographed, measured, and compared against a molecular size marker.\u003c\/p\u003e\n\u003cp\u003eThe characteristics that keep this material in continuous use are its high detection sensitivity toward very small quantities of DNA, the simplicity of the working procedure, and its compatibility with virtually every agarose gel electrophoresis system in circulation. The dye can be mixed directly into the gel before it sets, or it can be used as a post-electrophoresis soaking solution, so the workflow can be adapted to the established habits of each individual laboratory. The signal produced is stable, easy to read on a UV transilluminator as well as on digital gel documentation systems, and consistent from run to run.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, this reagent is a routine fixture wherever agarose gel electrophoresis is performed. It is used in university molecular biology teaching and research laboratories, in diagnostic and testing facilities that verify PCR products, and in institutional laboratories where DNA and RNA preparations must be checked before downstream work proceeds. Because the handling procedure is simple and the equipment required is already present in most gel rooms, the dye fits readily into existing routines without additional instrumentation.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAgarose gel electrophoresis of DNA — the dye binds to separated fragments and renders them as fluorescent bands under UV light, making band position and size comparison against a molecular weight marker possible.\u003c\/li\u003e\n\u003cli\u003eVerification of PCR products — amplified fragments can be confirmed for presence and approximate size directly on the gel, since the dye detects even very small quantities of DNA with high sensitivity.\u003c\/li\u003e\n\u003cli\u003eChecking RNA preparations — as an intercalator active on nucleic acids, the dye allows electrophoresed RNA samples to be inspected visually before further downstream procedures are carried out.\u003c\/li\u003e\n\u003cli\u003eGel documentation and imaging work — the stable fluorescent signal is read reliably by UV transilluminators and by digital gel documentation systems, so images can be photographed, stored, and compared.\u003c\/li\u003e\n\u003cli\u003eRoutine quality checks on extracted nucleic acids — laboratories use the dye to confirm that DNA or RNA isolates are intact and present before committing material to more demanding experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 1239-45-8\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Cell Culture \u0026gt; Cell Staining\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in the catalogue pack sizes offered by TCI for this item\u003c\/li\u003e\n\u003cli\u003ePhysical form: supplied as a nucleic acid staining reagent for agarose gel electrophoresis\u003c\/li\u003e\n\u003cli\u003eStorage: store according to the conditions stated on the product label and in the manufacturer's documentation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the material in its original, tightly closed container under the conditions indicated on the product label, protected from light and kept away from heat sources. Containers should be chemically compatible and clearly labelled, and stock solutions should be kept in sealed vessels within a designated storage area. Because this is a nucleic acid intercalating dye, handling should follow standard laboratory precautions: wear gloves, a laboratory coat, and eye protection, work in a well-ventilated area or designated gel station, and avoid direct skin contact with the powder or solution. Gels, solutions, gloves, and contaminated consumables should be segregated from ordinary laboratory waste and disposed of according to institutional chemical waste procedures. When reading gels, use appropriate shielding for UV exposure.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086840738010,"sku":"TCI2510E037028116","price":1363000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086840770778,"sku":"TCI2510E037028117","price":4518000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510E0370.jpg?v=1767111122"},{"product_id":"tci2510h134334163","title":"TCI H1343 23491-45-4 Bisbenzimide H 33258 [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eBisbenzimide H 33258, referred to in much of the literature as Hoechst 33258 stain, is a bisbenzimidazole-based fluorescent dye developed for biochemical and cell biology research. Its role in the laboratory is to label cell nuclei by binding to double-stranded DNA, so that nuclei which are essentially invisible under an ordinary light microscope become clearly readable under a fluorescence microscope. Because the dye is able to cross the cell membrane, it can be applied to living cells as well as to fixed cells, making it one of the most commonly encountered nuclear stains in cell culture protocols.\u003c\/p\u003e\n\u003cp\u003eThe principal characteristics behind its selection are its affinity for the minor groove of DNA, particularly in regions rich in adenine and thymine, together with the sharp increase in fluorescence intensity that follows binding to DNA. This behaviour produces good contrast between the nucleus and the background, so microscope images are easier to interpret and to count. Its emission falls in the blue range when excited with an ultraviolet source, which makes it straightforward to combine with other green or red markers in double-staining experiments.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, the material is typically used in cell culture facilities, biochemistry and molecular biology teaching laboratories, and university or institutional research units that operate a fluorescence microscope. It is handled in the same workflow as other cell staining reagents: prepared as a working solution, applied to cultured or fixed cells, then examined under ultraviolet excitation. Supplied by AMI Scientific as part of the TCI range for biochemical research, it fits routine nuclear staining work without requiring specialised instrumentation beyond a fluorescence setup.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine nuclear counterstaining in cell culture — the dye penetrates the membrane and binds double-stranded DNA, so nuclei of both living and fixed cells become clearly visible under fluorescence.\u003c\/li\u003e\n\u003cli\u003eCell counting and nuclear morphology assessment — the sharp rise in fluorescence intensity after DNA binding gives strong nucleus-to-background contrast, making counting and image interpretation more reliable.\u003c\/li\u003e\n\u003cli\u003eMulticolour double-staining experiments — blue emission under ultraviolet excitation separates cleanly from green and red markers, allowing the nuclear channel to be combined with other labels.\u003c\/li\u003e\n\u003cli\u003eLive-cell nuclear labelling studies — because the stain crosses the cell membrane, cells do not need to be fixed first, which suits work that must observe intact, viable cultures.\u003c\/li\u003e\n\u003cli\u003eBiochemical research on DNA binding — affinity for the minor groove, particularly adenine- and thymine-rich regions, makes the dye a useful probe in studies of double-stranded DNA interaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI (product code H1343)\u003c\/li\u003e\n\u003cli\u003eCAS Number: 23491-45-4\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Cell Culture \u0026gt; Cell Staining\u003c\/li\u003e\n\u003cli\u003eGrade \/ intended use: for biochemical research\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in the pack sizes listed for this catalogue item\u003c\/li\u003e\n\u003cli\u003eStorage: store according to the conditions stated on the manufacturer's label\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the container in the conditions indicated on the manufacturer's label, keeping it tightly closed and protected from light, since fluorescent dyes are generally sensitive to prolonged light exposure. Use clean, well-sealed containers of a material compatible with the reagent and its solvent, and label any working solutions clearly with their contents and preparation date. Handle the material in a properly ventilated area using standard personal protective equipment — laboratory coat, gloves, and eye protection — and avoid direct contact with skin and eyes. As with all DNA-binding stains used in research, handle the substance with care, avoid generating dust or aerosols, and dispose of residues and contaminated consumables through the laboratory's established chemical waste procedure. Consult the manufacturer's safety data sheet before first use.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"25mg","offer_id":48087277699290,"sku":"TCI2510H134334163","price":1389000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510H1343.jpg?v=1767117845"},{"product_id":"tci2510r020349701","title":"TCI R0203 62758-13-8 Resazurin Sodium Salt","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eResazurin Sodium Salt (TCI R0203), with CAS number 62758-13-8, is a chemical compound widely used as a stain and indicator in laboratory experiments. It plays a crucial role in various analytical procedures, particularly in detecting microbial activity and assessing reduction processes. Its ability to change color in response to reducing agents makes it an essential tool for researchers and scientists. This compound is commonly employed in environmental and microbiological studies to evaluate the presence of contaminants and the effectiveness of bioremediation processes. Its reliability and sensitivity make it a preferred choice for accurate and reproducible results in laboratory settings.\u003c\/p\u003e\n\u003cp\u003eResazurin Sodium Salt is known for its stability under specific conditions and its ability to undergo a visible color change, which simplifies the interpretation of experimental results. It is non-toxic at the concentrations typically used, ensuring a safe working environment. The compound is also resistant to moisture and light, enhancing its shelf life and usability. These properties make it an ideal choice for laboratories requiring precision and consistency in their analytical methods. Its versatility and ease of use further contribute to its popularity among researchers in various scientific fields.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, Resazurin Sodium Salt is extensively used in environmental, microbiological, and analytical chemistry research. It is a valuable resource for educational institutions and research centers due to its broad applications and ease of availability. Its role in both academic and industrial settings highlights its importance in advancing scientific knowledge and supporting practical experimentation.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eEnvironmental testing for microbial activity and water quality assessment due to its color-changing properties when reacting with reducing agents.\u003c\/li\u003e\n\u003cli\u003eMicrobiological research to detect and quantify microbial contamination in various samples such as soil, water, and food.\u003c\/li\u003e\n\u003cli\u003eBiochemical analysis to evaluate the presence of reducing substances in samples, aiding in the study of metabolic processes.\u003c\/li\u003e\n\u003cli\u003eEducational laboratories for teaching students about chemical indicators and their applications in experimental science.\u003c\/li\u003e\n\u003cli\u003eAnalytical chemistry for the development of assays and methods to assess the effectiveness of bioremediation processes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 62758-13-8\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Stains and Dyes (for Research and Experimental Use) \u0026gt; Stains and Dyes (Other) (for Research and Experimental Use)\u003c\/li\u003e\n\u003cli\u003ePack sizes: Available in various quantities as per supplier specifications\u003c\/li\u003e\n\u003cli\u003ePhysical form: Powder or crystalline solid\u003c\/li\u003e\n\u003cli\u003eStorage note: Store in a cool, dry place away from direct sunlight\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eResazurin Sodium Salt should be stored in a cool, dry environment to maintain its stability and effectiveness. It is recommended to keep the compound in airtight containers to prevent exposure to moisture and light, which can affect its properties. Laboratory personnel should handle the material with care, using appropriate personal protective equipment when necessary. The compound is non-toxic at typical usage levels, but standard safety protocols should be followed to ensure safe handling. Proper storage conditions help preserve the integrity of the material, ensuring reliable results in experimental applications.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48088212603098,"sku":"TCI2510R020349701","price":986000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48088212635866,"sku":"TCI2510R020349702","price":3257000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510R0203.jpg?v=1767138058"},{"product_id":"tci2510a24123050","title":"TCI A2412 28718-90-3 DAPI 2HCl [for Biochemical Research]","description":"\u003cp\u003e\u003cstrong\u003eDAPI 2HCl [for Biochemical Research]\u003c\/strong\u003e (CAS 28718-90-3) adalah produk kimia berkualitas tinggi dari TCI dengan grade \u003cstrong\u003eHPLC Grade\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAplikasi:\u003c\/strong\u003e Reagen sintesis untuk berbagai transformasi kimia organik di laboratorium dan industri.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpesifikasi:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eRumus Molekul: C16H15N5.2HCl\u003c\/li\u003e\n\u003cli\u003eCAS Number: 28718-90-3\u003c\/li\u003e\n\u003cli\u003eKemurnian: \u0026gt;90.0%(HPLC)\u003c\/li\u003e\n\u003cli\u003eKode Produk TCI: A2412\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eNama lain:\u003c\/strong\u003e 4',6-Diamidino-2-phenylindole Dihydrochloride; 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride; 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride\u003c\/p\u003e\u003cp\u003eTersedia di \u003cstrong\u003eAMI Scientific\u003c\/strong\u003e, distributor resmi TCI di Indonesia. \u003cstrong\u003eHubungi kami untuk penawaran harga dan ketersediaan stok\u003c\/strong\u003e — pengiriman ke seluruh Indonesia.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"5mg","offer_id":48085409562842,"sku":"TCI2510A24123050","price":1515000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510A2412.jpg?v=1767082817"}],"url":"https:\/\/amiscientific.com\/en\/collections\/tci-l4-disable-category-assays-and-reagents-for-measuring-cytotoxicity-proliferation-and-viability.oembed","provider":"AMI Scientific","version":"1.0","type":"link"}