{"title":"Functional Group Blocking Reagents","description":"\u003cp\u003e\u003cstrong\u003eFunctional Group Blocking Reagents\u003c\/strong\u003e — menghimpun reagen kecil yang bereaksi selektif dengan gugus fungsi tertentu, terutama tiol dan amina, untuk menonaktifkan sementara atau permanen reaktivitasnya sebelum tahap konjugasi lain dilangsungkan. Perannya melindungi atau memblokir gugus tersebut agar reaksi konjugasi berikutnya berjalan selektif pada situs yang diinginkan.\u003c\/p\u003e\u003cp\u003eReagen seperti N-Ethylmaleimide dan 2-Iodoacetamide umum dipakai peneliti biokimia untuk alkilasi gugus tiol bebas pada protein sebelum elektroforesis atau pemetaan ikatan disulfida, sementara Citraconic Anhydride dan Maleic Anhydride dipakai untuk asilasi reversibel gugus amina pada lisin. 4,4'-Dipyridyl Disulfide sering menjadi mitra reaksi pertukaran disulfida dalam studi konjugasi protein pada penelitian bioconjugation.\u003c\/p\u003e\u003cp\u003eContoh produk dalam koleksi ini: \u003ca href=\"\/en\/products\/tci2510m138239444\"\u003eTCI M1382 2949-92-0 S-Methyl Methanethiosulfonate\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510d111420833\"\u003eTCI D1114 2127-03-9 2,2'-Dipyridyl Disulfide [for Peptide Synthesis]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510m036537997\"\u003eTCI M0365 616-02-4 Citraconic Anhydride\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510d247722707\"\u003eTCI D2477 2645-22-9 4,4'-Dipyridyl Disulfide\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510m000537475\"\u003eTCI M0005 108-31-6 Maleic Anhydride\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510e013627811\"\u003eTCI E0136 128-53-0 N-Ethylmaleimide\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510i074135987\"\u003eTCI I0741 144-48-9 2-Iodoacetamide [for Biochemical Research]\u003c\/a\u003e.\u003c\/p\u003e\u003cp\u003ePerhatikan kemurnian dan bentuk fisik reagen (padatan kristal atau larutan), serta kestabilan terhadap hidrolisis karena sebagian anhidrida dan turunan iodoasetil peka terhadap kelembapan selama penyimpanan. Seluruh produk merupakan produk asli Tokyo Chemical Industry (TCI) Jepang, dengan kemurnian, kemasan, dan kondisi penyimpanan tercantum pada halaman masing-masing item.\u003c\/p\u003e\u003cp\u003eKategori lain yang sejajar di bawah Bioconjugation, sering dipakai bersamaan dalam satu alur kerja laboratorium:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-pegylation-reagents-and-peg-spacers\"\u003ePEGylation Reagents and PEG Spacers\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-biotins-and-streptavidins\"\u003eBiotins and Streptavidins\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-copper-free-click-reaction-reagents\"\u003eCopper-free Click Reaction Reagents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-functionality-creating-reagents\"\u003eFunctionality Creating Reagents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-zero-length-crosslinkers\"\u003eZero-length Crosslinkers\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-n-terminal-cysteine-residue-specific-conjugation-reagents\"\u003eN-Terminal Cysteine Residue-specific Conjugation Reagents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eKembali ke \u003ca href=\"\/en\/collections\/tci-l3-bioconjugation\"\u003eBioconjugation\u003c\/a\u003e. Untuk pengadaan volume besar, kemasan khusus, atau grade tertentu, hubungi tim AMI Scientific — distributor resmi TCI Japan di Indonesia — untuk pengecekan ketersediaan dan lead time langsung ke Jepang.\u003c\/p\u003e","products":[{"product_id":"tci2510e013627811","title":"TCI E0136 128-53-0 N-Ethylmaleimide","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eN-Ethylmaleimide with CAS number 128-53-0 is a classic reagent in the field of chemical biology, widely recognised for its ability to react specifically with thiol groups. The compound belongs to the maleimide family, characterised by an unsaturated ring that readily accepts nucleophilic attack through a Michael addition reaction. In routine biochemical laboratory practice, this reagent is used to block cysteine residues on proteins, allowing researchers to establish the role of sulfhydryl groups in enzyme activity, protein structure, and the biological reaction pathways under investigation in a systematic and controlled manner.\u003c\/p\u003e\n\u003cp\u003eThe property that makes N-Ethylmaleimide so dependable is the selectivity of its reaction toward thiols in the near-neutral pH range, which allows modification to proceed without disturbing other functional groups present on the biomolecule. The thioether bond that is formed is stable, so the modified product can be carried forward into further analysis without concern that the reaction will reverse. Its small molecular size means the reagent penetrates readily and can reach cysteine residues that are comparatively shielded. These characteristics are precisely what have made it a benchmark for comparison in many bioconjugation protocols and enzyme inhibition studies.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, N-Ethylmaleimide is typically found in academic biochemistry and molecular biology laboratories, university research groups, and analytical or quality-control laboratories that work with proteins and enzymes. It is generally used at the bench scale in method development and teaching contexts, where a well-documented and predictable thiol-blocking reagent is required so that experimental results remain comparable with published protocols.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCysteine residue blocking in proteins — the reagent reacts specifically with free sulfhydryl groups, allowing researchers to mask reactive cysteines before further treatment and confirm their contribution to protein function.\u003c\/li\u003e\n\u003cli\u003eEnzyme inhibition studies — because many enzymes depend on an active-site cysteine, controlled reaction with this maleimide helps establish whether sulfhydryl groups are essential to catalytic activity under the conditions studied.\u003c\/li\u003e\n\u003cli\u003eBioconjugation protocol development — the stable thioether bond formed and the predictable near-neutral pH selectivity make this compound a common benchmark against which newer thiol-reactive linkers are compared.\u003c\/li\u003e\n\u003cli\u003eInvestigation of protein structure and folding — selective modification of cysteine residues lets researchers probe which sulfhydryl groups are exposed and which remain shielded within the folded structure.\u003c\/li\u003e\n\u003cli\u003eStudies of biological reaction pathways — by removing free thiols from the system in a controlled way, investigators can determine which steps of a biochemical pathway genuinely depend on sulfhydryl chemistry.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 128-53-0\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Chemical Biology \u0026gt; Bioconjugation\u003c\/li\u003e\n\u003cli\u003eProduct code: E0136\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in standard TCI laboratory-scale research packaging; please confirm the currently listed pack options when ordering.\u003c\/li\u003e\n\u003cli\u003eStorage: store according to the manufacturer's stated conditions on the product label and safety data sheet.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the container tightly closed in a cool, dry, well-ventilated place, away from moisture, heat, and incompatible materials, following the conditions stated by the manufacturer on the label and safety data sheet. Keep the material in its original chemically compatible container, and use clean, dry glassware or plasticware when preparing working solutions. As with any thiol-reactive laboratory chemical, handle it inside a fume hood where practical, wear a laboratory coat, safety glasses, and suitable gloves, and avoid contact with skin, eyes, and inhalation of dust. Prepare solutions fresh where the protocol requires it, label all containers clearly, and dispose of residues and contaminated consumables in line with your institution's chemical waste procedures.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086819799258,"sku":"TCI2510E013627811","price":564000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086819832026,"sku":"TCI2510E013627812","price":1153000.0,"currency_code":"IDR","in_stock":true},{"title":"25g","offer_id":48086819864794,"sku":"TCI2510E013627813","price":3064000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510E0136.jpg?v=1767110727"},{"product_id":"tci2510m000537475","title":"TCI M0005 108-31-6 Maleic Anhydride","description":"\u003cp\u003e\u003cstrong\u003eMaleic Anhydride\u003c\/strong\u003e (CAS 108-31-6) is a high-quality chemical from TCI, supplied as \u003cstrong\u003eHigh Purity Reagent Grade\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e Synthesis reagent for a wide range of organic chemistry transformations in laboratory and industrial settings.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eMolecular formula: C4H2O3\u003c\/li\u003e\n\u003cli\u003eCAS number: 108-31-6\u003c\/li\u003e\n\u003cli\u003ePurity: \u0026gt;99.0%(GC)(T)\u003c\/li\u003e\n\u003cli\u003eTCI product code: M0005\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAvailable from \u003cstrong\u003eAMI Scientific\u003c\/strong\u003e, the authorised TCI distributor in Indonesia. \u003cstrong\u003eContact us for pricing and stock availability\u003c\/strong\u003e — we ship throughout Indonesia.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"25g","offer_id":48087503601882,"sku":"TCI2510M000537475","price":479000.0,"currency_code":"IDR","in_stock":true},{"title":"500g","offer_id":48087503634650,"sku":"TCI2510M000537476","price":620000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510M0005.jpg?v=1767121988"},{"product_id":"tci2510m036537997","title":"TCI M0365 616-02-4 Citraconic Anhydride","description":"\u003cp\u003e\u003cstrong\u003eCitraconic Anhydride\u003c\/strong\u003e (CAS 616-02-4) is a high-quality chemical from TCI, supplied as \u003cstrong\u003eGC Grade \/ for Synthesis\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e Synthesis reagent for a wide range of organic chemistry transformations in laboratory and industrial settings.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eMolecular formula: C5H4O3\u003c\/li\u003e\n\u003cli\u003eCAS number: 616-02-4\u003c\/li\u003e\n\u003cli\u003ePurity: \u0026gt;98.0%(GC)\u003c\/li\u003e\n\u003cli\u003eTCI product code: M0365\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSynonyms:\u003c\/strong\u003e Methylmaleic Anhydride\u003c\/p\u003e\u003cp\u003eAvailable from \u003cstrong\u003eAMI Scientific\u003c\/strong\u003e, the authorised TCI distributor in Indonesia. \u003cstrong\u003eContact us for pricing and stock availability\u003c\/strong\u003e — we ship throughout Indonesia.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"25g","offer_id":48087533355226,"sku":"TCI2510M036537997","price":507000.0,"currency_code":"IDR","in_stock":true},{"title":"100g","offer_id":48087533387994,"sku":"TCI2510M036537998","price":1322000.0,"currency_code":"IDR","in_stock":true},{"title":"500g","offer_id":48087533420762,"sku":"TCI2510M036537999","price":3993000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510M0365.jpg?v=1767122762"}],"url":"https:\/\/amiscientific.com\/en\/collections\/tci-l4-functional-group-blocking-reagents.oembed","provider":"AMI Scientific","version":"1.0","type":"link"}