{"title":"Nucleoside Azides\/Alkynes [Conjugation Chemistry\/Click Chemistry]","description":"\u003cp\u003e\u003cstrong\u003eNucleoside Azides\/Alkynes [Conjugation Chemistry\/Click Chemistry]\u003c\/strong\u003e — berisi nukleosida termodifikasi yang membawa gugus azida atau alkuna terminal pada posisi gula atau basa. Golongan ini berperan sebagai bahan baku reaksi cycloaddition azida-alkuna terkatalisis tembaga (klik kimia) untuk konjugasi biomolekul.\u003c\/p\u003e\u003cp\u003eTurunan seperti 5-etinilurasil dan analog azido-deoksinukleosida dimanfaatkan untuk pelabelan DNA atau RNA yang baru disintesis dalam sel melalui inkorporasi metabolik, diikuti reaksi klik dengan pasangan berlabel fluoresen atau biotin. Peneliti biologi molekular dan pengembang probe pelabelan asam nukleat menggunakan golongan ini untuk visualisasi replikasi DNA maupun deteksi sintesis RNA baru pada mikroskopi dan sitometri.\u003c\/p\u003e\u003cp\u003eContoh produk dalam koleksi ini: \u003ca href=\"\/en\/products\/tci2510a29423688\"\u003eTCI A2942 26929-65-7 2'-Azido-2'-deoxyuridine\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510e105728939\"\u003eTCI E1057 61135-33-9 5-Ethynyl-2'-deoxyuridine\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510a20522606\"\u003eTCI A2052 30516-87-1 3'-Azido-3'-deoxythymidine\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510e156029612\"\u003eTCI E1560 69075-42-9 5-Ethynyluridine\u003c\/a\u003e.\u003c\/p\u003e\u003cp\u003ePilih posisi modifikasi (gula atau basa) dan jenis gugus reaktif (azida atau alkuna) yang sesuai dengan pasangan reaksi klik serta kompatibilitas dengan enzim polimerase pada aplikasi inkorporasi metabolik. Seluruh produk merupakan produk asli Tokyo Chemical Industry (TCI) Jepang, dengan kemurnian, kemasan, dan kondisi penyimpanan tercantum pada halaman masing-masing item.\u003c\/p\u003e\u003cp\u003eKategori lain yang sejajar di bawah Conjugation Chemistry\/Click Chemistry, sering dipakai bersamaan dalam satu alur kerja laboratorium:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-terminal-alkynes-conjugation-chemistry-click-chemistry\"\u003eTerminal Alkynes [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-azides-conjugation-chemistry-click-chemistry\"\u003eAzides [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-crosslinker-azides-alkynes-conjugation-chemistry-click-chemistry\"\u003eCrosslinker Azides\/Alkynes [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-catalysts-ligands-reagents-conjugation-chemistry-click-chemistry\"\u003eCatalysts, Ligands, Reagents [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-tetrazines-conjugation-chemistry-click-chemistry\"\u003eTetrazines [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-peg-azides-alkynes-conjugation-chemistry-click-chemistry\"\u003ePEG Azides\/Alkynes [Conjugation Chemistry\/Click Chemistry]\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eKembali ke \u003ca href=\"\/en\/collections\/tci-l3-conjugation-chemistry-click-chemistry\"\u003eConjugation Chemistry\/Click Chemistry\u003c\/a\u003e. Untuk pengadaan volume besar, kemasan khusus, atau grade tertentu, hubungi tim AMI Scientific — distributor resmi TCI Japan di Indonesia — untuk pengecekan ketersediaan dan lead time langsung ke Jepang.\u003c\/p\u003e","products":[{"product_id":"tci2510e105728939","title":"TCI E1057 61135-33-9 5-Ethynyl-2'-deoxyuridine","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003e5-Ethynyl-2'-deoxyuridine (EdU) is a chemical compound widely used in molecular biology and biomedical research to directly monitor cell proliferation within a living environment. This compound is capable of incorporating into the DNA of dividing cells, making it an effective tool for identifying actively cycling cells. EdU is particularly valuable in studies that require real-time observation of cellular processes, such as cell growth, differentiation, and apoptosis. Its ability to integrate into DNA during replication allows researchers to track cell division with high specificity and accuracy.\u003c\/p\u003e\n\u003cp\u003eEdU is preferred due to its chemical stability, reactivity, and efficient cellular uptake. Its structure closely resembles a DNA nucleotide, enabling it to be incorporated into DNA during replication. This feature makes it ideal for use in conjugation chemistry, especially in click chemistry techniques, which allow for the specific and rapid attachment of other molecules to DNA. The compound's reactivity and compatibility with various detection methods further enhance its utility in laboratory settings.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, EdU is commonly used in applied research, including studies on cell growth, drug response, and diagnostic technique development. Its application supports a wide range of biological investigations, from cancer research to tissue regeneration studies. The compound's reliability and effectiveness make it a key reagent in both academic and industrial research environments.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCell proliferation studies benefit from EdU’s ability to label dividing cells, providing insights into cell cycle dynamics and growth patterns.\u003c\/li\u003e\n\u003cli\u003eCancer research utilizes EdU to monitor tumor cell proliferation and assess the effectiveness of therapeutic interventions.\u003c\/li\u003e\n\u003cli\u003eTissue regeneration studies rely on EdU to track cell division in regenerating tissues, aiding in the understanding of repair mechanisms.\u003c\/li\u003e\n\u003cli\u003eDrug response analysis employs EdU to evaluate how cells respond to various treatments by measuring changes in proliferation rates.\u003c\/li\u003e\n\u003cli\u003eDiagnostic technique development incorporates EdU to create new methods for detecting and analyzing cellular activity in clinical samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 61135-33-9\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Chemical Biology \u0026gt; Conjugation Chemistry\/Click Chemistry\u003c\/li\u003e\n\u003cli\u003ePack sizes: Available in various quantities as per standard laboratory reagent packaging\u003c\/li\u003e\n\u003cli\u003ePhysical form: Solid powder\u003c\/li\u003e\n\u003cli\u003eStorage note: Store in a cool, dry place away from light\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eEdU should be stored in a cool, dry environment, away from direct light to maintain its chemical stability. It is recommended to use airtight containers to prevent moisture absorption, which could affect its reactivity. When handling EdU, laboratory personnel should wear appropriate personal protective equipment, such as gloves and safety goggles, to avoid skin or eye contact. The compound is generally non-toxic but should be handled with care as with any chemical reagent. Proper labeling of storage containers is essential to ensure safe access and prevent accidental exposure. Regular monitoring of storage conditions ensures the compound remains effective for its intended applications in research.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"50mg","offer_id":48086920593626,"sku":"TCI2510E105728939","price":1687000.0,"currency_code":"IDR","in_stock":true},{"title":"200mg","offer_id":48086920626394,"sku":"TCI2510E105728940","price":4919000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510E1057.jpg?v=1767112255"},{"product_id":"tci2510e156029612","title":"TCI E1560 69075-42-9 5-Ethynyluridine","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003e5-Ethynyluridine, commonly abbreviated as EU, is a uridine nucleoside analogue that carries an ethynyl group at the 5 position of the uracil ring. The compound is a key reagent in molecular biology research for labelling newly synthesised RNA inside living cells. Its working principle is simple yet elegant: cells supplied with EU incorporate the analogue into RNA that is actively being transcribed, and the ethynyl group embedded within that RNA is then detected through a click reaction with an azide-labelled dye. The result is a picture of transcriptional activity that can be visualised directly.\u003c\/p\u003e\n\u003cp\u003eThe advantages that make EU the reagent of choice lie in the very small size of the ethynyl group, which keeps interference with biological processes to a minimum, and in the selectivity of the click reaction, which barely touches other functional groups present in the cell. This approach replaces older methods based on radiolabelled nucleosides or antibodies, because it requires no handling of radioactive material and demands no harsh denaturation to open access for the probe. Click-based detection also produces sharp signals with low background, making it suitable for both fluorescence microscopy and flow cytometry analysis.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, EU is typically used in university research groups, biomedical and molecular biology institutes, and cell culture facilities that study gene expression and transcription dynamics. It fits workflows where fluorescence microscopy or flow cytometry is already established, and where investigators need a non-radioactive route to visualising nascent RNA. Supplied by TCI under catalogue number E1560, it is handled alongside the usual click chemistry reagents such as azide-labelled fluorescent dyes.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eNascent RNA labelling in cultured cells: EU is incorporated into RNA during active transcription, allowing newly synthesised transcripts to be distinguished from the pre-existing RNA pool without radioactive tracers.\u003c\/li\u003e\n\u003cli\u003eTranscriptional activity imaging by fluorescence microscopy: the click reaction with azide-labelled dyes yields sharp signals and low background, so sites of active transcription can be visualised directly within the cell.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis of transcription: click-based detection of EU-labelled RNA gives clean, quantifiable fluorescence across large cell populations, supporting comparison of transcriptional activity between samples.\u003c\/li\u003e\n\u003cli\u003eClick chemistry and conjugation chemistry method development: the ethynyl handle reacts selectively with azide partners and barely touches other cellular functional groups, making EU a dependable model substrate for protocol optimisation.\u003c\/li\u003e\n\u003cli\u003eGene expression studies as an alternative to antibody or radiolabel methods: no radioactive handling is required and no harsh denaturation is needed to give the probe access to the target RNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 69075-42-9\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Chemical Biology \u0026gt; Conjugation Chemistry \/ Click Chemistry\u003c\/li\u003e\n\u003cli\u003eCatalogue number: E1560\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in standard research-scale pack sizes; please confirm the size required when ordering.\u003c\/li\u003e\n\u003cli\u003eStorage: store according to the conditions stated on the manufacturer's label and product documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the material in its original tightly closed container, following the storage conditions stated on the manufacturer's label and safety data sheet. Keep the container sealed and protected from moisture, and return it promptly to the designated storage location after each use. Weigh and dispense in a clean, well-ventilated working area, and prepare solutions using appropriate solvent and labware for cell culture work. Wear standard laboratory personal protective equipment, including gloves, a laboratory coat, and eye protection, and avoid direct contact with skin, eyes, and clothing. Use only clean, dry spatulas to prevent contamination of the remaining stock, and record the opening date on the container. Dispose of residues and contaminated consumables in accordance with the applicable laboratory chemical waste procedures. Consult the manufacturer's safety data sheet before first use.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"50mg","offer_id":48086969680090,"sku":"TCI2510E156029612","price":2053000.0,"currency_code":"IDR","in_stock":true},{"title":"250mg","offer_id":48086969712858,"sku":"TCI2510E156029613","price":5876000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510E1560.jpg?v=1767113063"},{"product_id":"tci2510a20522606","title":"TCI A2052 30516-87-1 3'-Azido-3'-deoxythymidine","description":"\u003cp\u003e\u003cstrong\u003e3'-Azido-3'-deoxythymidine\u003c\/strong\u003e (CAS 30516-87-1) is a high-quality chemical from TCI, supplied as \u003cstrong\u003eReagent Grade \/ for Synthesis\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e Used as a building block in oligonucleotide synthesis and biochemical research.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eMolecular formula: C10H13N5O4\u003c\/li\u003e\n\u003cli\u003eCAS number: 30516-87-1\u003c\/li\u003e\n\u003cli\u003ePurity: \u0026gt;98.0%(T)\u003c\/li\u003e\n\u003cli\u003eTCI product code: A2052\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSynonyms:\u003c\/strong\u003e Azidothymidine; AZT; 1-(3-Azido-2,3-dideoxy-beta-D-ribofuranosyl)thymine; Zidovudine\u003c\/p\u003e\u003cp\u003eAvailable from \u003cstrong\u003eAMI Scientific\u003c\/strong\u003e, the authorised TCI distributor in Indonesia. \u003cstrong\u003eContact us for pricing and stock availability\u003c\/strong\u003e — we ship throughout Indonesia.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48085390885082,"sku":"TCI2510A20522606","price":2559000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48085390917850,"sku":"TCI2510A20522607","price":7336000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510A2052.jpg?v=1768192979"},{"product_id":"tci2510a29423688","title":"TCI A2942 26929-65-7 2'-Azido-2'-deoxyuridine","description":"\u003cp\u003e\u003cstrong\u003e2'-Azido-2'-deoxyuridine\u003c\/strong\u003e (CAS 26929-65-7) is a high-quality chemical from TCI, supplied as \u003cstrong\u003eHPLC Grade\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e Used as a building block in oligonucleotide synthesis and biochemical research.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eMolecular formula: C9H11N5O5\u003c\/li\u003e\n\u003cli\u003eCAS number: 26929-65-7\u003c\/li\u003e\n\u003cli\u003ePurity: \u0026gt;98.0%(HPLC)\u003c\/li\u003e\n\u003cli\u003eTCI product code: A2942\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSynonyms:\u003c\/strong\u003e N3-dU\u003c\/p\u003e\u003cp\u003eAvailable from \u003cstrong\u003eAMI Scientific\u003c\/strong\u003e, the authorised TCI distributor in Indonesia. \u003cstrong\u003eContact us for pricing and stock availability\u003c\/strong\u003e — we ship throughout Indonesia.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"100mg","offer_id":48085442003162,"sku":"TCI2510A29423688","price":5369000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510A2942.jpg?v=1767083593"}],"url":"https:\/\/amiscientific.com\/en\/collections\/tci-l4-nucleoside-azides-alkynes-conjugation-chemistry-click-chemistry.oembed","provider":"AMI Scientific","version":"1.0","type":"link"}