{"title":"Substrates, Buffers, Blockers [Antibody Related Products]","description":"\u003cp\u003e\u003cstrong\u003eSubstrates, Buffers, Blockers [Antibody Related Products]\u003c\/strong\u003e — menghimpun senyawa kromogenik dan fluorogenik seperti 3,3',5,5'-Tetramethylbenzidine, Blue Tetrazolium, dan ADHP yang menghasilkan perubahan warna atau pendaran saat bereaksi dengan enzim penanda. Golongan ini digunakan sebagai substrat pendukung dalam sistem deteksi berbasis antibodi seperti ELISA atau imunoblotting.\u003c\/p\u003e\u003cp\u003eSubstrates, Buffers, Blockers untuk produk terkait antibodi dipakai peneliti imunologi dan biologi molekuler sebagai substrat enzim pelapor seperti horseradish peroxidase atau alkaline phosphatase pada uji ELISA, Western blot, maupun histokimia. 3,3',5,5'-Tetramethylbenzidine umum digunakan sebagai substrat kromogenik peroksidase, 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt dipakai pada deteksi aktivitas fosfatase alkali, sedangkan garam tetrazolium seperti Blue Tetrazolium dan 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride berperan dalam uji viabilitas atau deteksi aktivitas dehidrogenase.\u003c\/p\u003e\u003cp\u003eContoh produk dalam koleksi ini: \u003ca href=\"\/en\/products\/tci2510a0257366\"\u003eTCI A0257 22198-72-7 4-Aminoantipyrine Hydrochloride [for Biochemical Research]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510c229117021\"\u003eTCI C2291 604-44-4 4-Chloro-1-naphthol [for Biochemical Research]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510i078136040\"\u003eTCI I0781 146-68-9 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride [for Biochemical Research]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510c338418427\"\u003eTCI C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510t257353940\"\u003eTCI T2573 54827-17-7 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510c408719199\"\u003eTCI C4087 Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510b35819533\"\u003eTCI B3581 1871-22-3 Blue Tetrazolium [for Biochemical Research]\u003c\/a\u003e, \u003ca href=\"\/en\/products\/tci2510d084420483\"\u003eTCI D0844 298-83-9 Nitro Blue Tetrazolium [for Biochemical Research]\u003c\/a\u003e.\u003c\/p\u003e\u003cp\u003ePemilihan perlu disesuaikan dengan jenis enzim pelapor yang digunakan dalam sistem deteksi, panjang gelombang produk warna atau fluoresensi yang dihasilkan, serta kompatibilitas reagen dengan protokol pewarnaan yang berlaku. Seluruh produk merupakan produk asli Tokyo Chemical Industry (TCI) Jepang, dengan kemurnian, kemasan, dan kondisi penyimpanan tercantum pada halaman masing-masing item.\u003c\/p\u003e\u003cp\u003eKategori lain yang sejajar di bawah Antibody Supplementary Products, sering dipakai bersamaan dalam satu alur kerja laboratorium:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-antibody-modification\"\u003eAntibody Modification\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-streptavidins\"\u003eStreptavidins\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-protein-a\"\u003eProtein A\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-protein-g\"\u003eProtein G\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/en\/collections\/tci-l4-protein-l\"\u003eProtein L\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eKembali ke \u003ca href=\"\/en\/collections\/tci-l3-antibody-supplementary-products\"\u003eAntibody Supplementary Products\u003c\/a\u003e. Untuk pengadaan volume besar, kemasan khusus, atau grade tertentu, hubungi tim AMI Scientific — distributor resmi TCI Japan di Indonesia — untuk pengecekan ketersediaan dan lead time langsung ke Jepang.\u003c\/p\u003e","products":[{"product_id":"tci2510c229117021","title":"TCI C2291 604-44-4 4-Chloro-1-naphthol [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003e4-Chloro-1-naphthol is a chemical compound widely used in biochemical research. It is a key reagent in laboratory settings where precise chemical reactions are required. This compound features an aromatic structure with a chlorine substituent at the C-4 position, which enhances its reactivity in various chemical processes. Its versatility makes it a valuable tool for researchers working on enzyme reactions and analytical methods. Due to its stability and reactivity, it is frequently used in experiments that demand accuracy and reproducibility.\u003c\/p\u003e\n\u003cp\u003eThe compound's chemical properties make it a preferred choice for laboratory applications. It exhibits solubility in organic solvents such as ethyl acetate and ethanol, allowing for easy incorporation into reaction mixtures. Its ability to participate in electrophilic substitution reactions further expands its utility in synthetic chemistry. Additionally, its resistance to degradation under standard laboratory conditions ensures long-term stability and reliability of experimental results. These characteristics make it a reliable and effective reagent for biochemical research.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, 4-Chloro-1-naphthol is commonly used in biochemical research, including enzyme studies and analytical method development. Its role is crucial in experiments that require precise chemical interactions and stable reagents. Researchers in Indonesia rely on this compound for its consistent performance and compatibility with various experimental protocols. Its widespread use highlights its importance in both academic and industrial research settings.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme Kinetics Studies: This compound is ideal for enzyme reaction experiments due to its reactivity and stability, allowing accurate measurement of enzyme activity.\u003c\/li\u003e\n\u003cli\u003eQuantitative Analysis Development: Its solubility and chemical behavior make it suitable for creating reagents used in quantitative testing methods.\u003c\/li\u003e\n\u003cli\u003eSynthetic Organic Chemistry: The compound's ability to undergo electrophilic substitution reactions makes it a valuable reagent in organic synthesis.\u003c\/li\u003e\n\u003cli\u003eBiochemical Assay Preparation: It is commonly used in preparing substrates for biochemical assays that require controlled chemical reactions.\u003c\/li\u003e\n\u003cli\u003eSpectroscopic Analysis: Its distinct chemical properties make it useful in spectroscopic studies for identifying and analyzing molecular structures.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS Number: 604-44-4\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Antibodies \u0026gt; Antibody Supplementary Products\u003c\/li\u003e\n\u003cli\u003ePack Sizes: Available in various quantities as per standard laboratory supply\u003c\/li\u003e\n\u003cli\u003ePhysical Form: Solid, crystalline\u003c\/li\u003e\n\u003cli\u003eStorage Note: Store in a cool, dry place away from light\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eThis compound should be stored in a cool, dry place away from direct light to maintain its stability and effectiveness. It is recommended to use airtight containers to prevent moisture absorption, which can affect its chemical properties. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. It is important to ensure good ventilation when working with this material to avoid inhalation of vapors. Proper storage and handling practices help preserve the compound's integrity and ensure safe laboratory operations.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086117777626,"sku":"TCI2510C229117021","price":732000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086117810394,"sku":"TCI2510C229117022","price":1997000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510C2291.jpg?v=1767098083"},{"product_id":"tci2510d375624119","title":"TCI D3756 91-95-2 3,3'-Diaminobenzidine [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003e3,3'-Diaminobenzidine, commonly abbreviated as DAB, is a tetra-amino aromatic compound prepared for biochemical research purposes. Its molecule consists of two directly linked benzene rings, each carrying two amino groups, which provides four nucleophilic centres together with a readily oxidised pi-electron system. Its principal role in the laboratory is as a chromogenic substrate in reactions catalysed by peroxidase enzymes, where DAB is oxidised and precipitates as an insoluble brown product exactly at the site of the enzyme. In addition, this tetra-amino framework is also used as a monomer and building block in heterocyclic synthesis.\u003c\/p\u003e\n\u003cp\u003eThe characteristics that make DAB such a widely chosen reagent are the stability, contrast, and strong adherence of its oxidation precipitate to tissues or membranes. The brown colour that forms is insoluble in both water and alcohol, so preparations can be dehydrated, cleared, and mounted in a permanent medium without any loss of signal. It is precisely this property that allows stained preparations to be archived and re-examined years later. As a free base, the compound is generally dissolved first in a suitable solvent before use in the working system.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, this material is typically handled in biochemistry, molecular biology, histology, and pathology work, where peroxidase-based detection is a routine part of the workflow. It is also drawn on by organic and materials synthesis groups that require a non-heterocyclic building block with multiple amino centres. Supplied as a TCI catalogue item under the Chemistry \u0026gt; Building Blocks \u0026gt; Non-Heterocyclic Building Blocks category, it is ordered by university research units, hospital laboratories, and industrial R\u0026amp;D facilities alike.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eImmunohistochemistry (IHC) detection: DAB oxidised by horseradish peroxidase deposits an insoluble brown precipitate directly at the antigen site, giving sharp, high-contrast localisation on tissue sections.\u003c\/li\u003e\n\u003cli\u003eWestern blot membrane development: the precipitate adheres firmly to the blotting membrane and does not wash away, producing permanent visible bands that require no imaging equipment to read.\u003c\/li\u003e\n\u003cli\u003eImmunocytochemistry on cultured cells: because the coloured product is insoluble in water and alcohol, stained cell preparations survive dehydration and clearing without any loss of signal intensity.\u003c\/li\u003e\n\u003cli\u003eEnzyme histochemistry and peroxidase activity mapping: the reaction proceeds precisely where the enzyme is located, so endogenous or introduced peroxidase activity can be mapped with good spatial fidelity.\u003c\/li\u003e\n\u003cli\u003eHeterocyclic and polymer synthesis: the four amino groups on a directly linked biphenyl framework make this a versatile monomer and building block for condensation into heterocyclic ring systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI (Tokyo Chemical Industry), catalogue number D3756\u003c\/li\u003e\n\u003cli\u003eCAS number: 91-95-2\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Building Blocks \u0026gt; Non-Heterocyclic Building Blocks\u003c\/li\u003e\n\u003cli\u003eGrade: [for Biochemical Research]\u003c\/li\u003e\n\u003cli\u003eChemical form: free base of the tetra-amino aromatic compound, dissolved in a suitable solvent before use\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in the standard TCI research pack sizes; storage conditions follow the manufacturer's label\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the container tightly closed in a cool, dry place away from direct sunlight, heat sources, and strong oxidising agents, following the storage conditions stated on the manufacturer's label. Amber glass or the original supplier container is preferred, since the compound is readily oxidised and light exposure should be minimised. Handle weighing and dissolution inside a fume hood, and wear a laboratory coat, nitrile gloves, and eye protection throughout. Avoid raising dust, and do not inhale or allow contact with skin. Prepare working solutions fresh where possible and keep them protected from light. Collect residues and used solutions as designated chemical waste for disposal in accordance with applicable institutional and local regulations.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086636200154,"sku":"TCI2510D375624119","price":788000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086636232922,"sku":"TCI2510D375624120","price":2306000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510D3756.jpg?v=1767106510"},{"product_id":"tci2510d375724121","title":"TCI D3757 868272-85-9 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eTCI D3757 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate is the hydrated tetrahydrochloride salt form of DAB, prepared specifically for biochemical research and classified within antibody supplementary products. Like its free base counterpart, this compound functions as a chromogenic substrate for peroxidase enzymes, particularly horseradish peroxidase conjugated to secondary antibodies. When the enzyme acts in the presence of hydrogen peroxide, DAB is oxidised and precipitates as an insoluble brown reaction product, allowing the position of an antigen in tissue or a protein band on a membrane to be observed directly by eye or under an ordinary light microscope.\u003c\/p\u003e\n\u003cp\u003eThe principal advantage of the tetrahydrochloride salt form lies in its ease of handling. As a salt, this compound is considerably more soluble in water and aqueous buffers than the free base, so working solutions can be prepared quickly without the need for additional organic solvents. The salt form is also generally more stable against oxidation during storage, which reduces the risk of the material darkening before it is used. The properties of the final reaction product remain equally advantageous: the brown precipitate that forms adheres strongly and resists dehydration and subsequent processing steps.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, this product is typically used wherever peroxidase-based antibody detection is routine. It serves immunohistochemistry benches in hospital and university pathology units, research groups working on tissue localisation studies, and laboratories performing membrane-based protein detection. Because the salt dissolves readily in aqueous buffer, it suits facilities that prefer straightforward reagent preparation, and the stable brown product allows slides and membranes to be archived and re-examined long after the staining run is complete.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eImmunohistochemistry on tissue sections — the insoluble brown precipitate marks antigen position directly on the slide and remains visible under an ordinary light microscope without specialised optics.\u003c\/li\u003e\n\u003cli\u003eWestern blot and membrane-based protein detection — DAB oxidation produces a permanent brown band on the membrane, giving a visual record of the target protein that needs no imaging equipment.\u003c\/li\u003e\n\u003cli\u003eHorseradish peroxidase conjugate detection systems — the compound acts as the chromogenic substrate for HRP linked to secondary antibodies, completing the enzyme-based signal generation step.\u003c\/li\u003e\n\u003cli\u003eRoutine diagnostic and teaching histology preparations — because the reaction product resists dehydration and further processing, stained slides can be mounted, archived and reviewed repeatedly.\u003c\/li\u003e\n\u003cli\u003eGeneral biochemical research on peroxidase activity — the salt dissolves quickly in aqueous buffer, so working solutions for enzyme-linked assays can be prepared without organic solvents.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 868272-85-9\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Antibodies \u0026gt; Antibody Supplementary Products\u003c\/li\u003e\n\u003cli\u003eProduct code: D3757\u003c\/li\u003e\n\u003cli\u003eChemical form: tetrahydrochloride salt, hydrate; grade stated for biochemical research\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in the pack sizes offered by the manufacturer for this catalogue item\u003c\/li\u003e\n\u003cli\u003eStorage: store as directed for the product; keep protected from light and air to limit oxidation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eKeep the container tightly closed and store it in a cool, dark place, since DAB salts darken on exposure to light and air. Original manufacturer packaging or amber glass with a well-sealed cap is suitable; transfer only the quantity needed for immediate use and reseal promptly. Prepare working solutions fresh in aqueous buffer and handle them in a fume hood where practicable. Wear gloves, a laboratory coat and eye protection, avoid skin contact and inhalation of the powder, and collect DAB-containing waste separately for disposal according to institutional chemical waste procedures.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086636265690,"sku":"TCI2510D375724121","price":1350000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086636298458,"sku":"TCI2510D375724122","price":3598000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510D3757.jpg?v=1767106514"},{"product_id":"tci2510d393124365","title":"TCI D3931 536-46-9 N,N-Dimethyl-1,4-phenylenediamine Dihydrochloride [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eTCI D3931 N,N-Dimethyl-1,4-phenylenediamine Dihydrochloride [for Biochemical Research] is the dihydrochloride salt of a para-phenylenediamine derivative that carries two methyl groups on one of its nitrogen atoms. The compound is a classic chromogenic reagent, and its role in the laboratory is to develop colour when it undergoes oxidation or reacts with certain analytes, so that the presence of a substance that was originally colourless can be followed visually or measured quantitatively with a spectrophotometer. This product class is categorised as a supplementary material for biochemical research and antibody-based work.\u003c\/p\u003e\n\u003cp\u003eThe property that makes this reagent a preferred choice is the ease with which it is oxidised into a strongly coloured species, combined with its good solubility in water thanks to the dihydrochloride salt form. The salt form also makes the powder more stable to handle and weigh than the free base, which darkens in air far more readily. Because the response is sensitive and relatively rapid, the reagent is suited both to qualitative bench-top tests and to colorimetric methods that require absorbance readings. The electron-donating character of the dimethylamino group is what underlies this colour formation behaviour.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, this reagent fits routine analytical and biochemical research work where a colour-forming indicator is needed to confirm or measure a reaction. It is typically used in university research laboratories, biochemistry and life science units, and analytical laboratories that run colorimetric procedures. Because it serves as a supplementary product for antibody-related work, it is generally kept on hand as part of the reagent stock supporting detection and staining workflows rather than as a bulk consumable.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eColorimetric assay development: the reagent forms a strongly coloured oxidation product, allowing colourless analytes to be quantified by absorbance measurement on a standard laboratory spectrophotometer.\u003c\/li\u003e\n\u003cli\u003eQualitative bench-top screening tests: because the colour response is sensitive and relatively rapid, results can be judged visually without instrumentation during preliminary or routine confirmation work.\u003c\/li\u003e\n\u003cli\u003eAntibody-based detection support: it is classified as an antibody supplementary product, so it serves as the colour-generating component in detection systems built around antibody reagents.\u003c\/li\u003e\n\u003cli\u003eOxidation-based indicator chemistry: the electron-donating dimethylamino group makes the molecule readily oxidised, so it acts as a reliable visual indicator of oxidising conditions in a reaction system.\u003c\/li\u003e\n\u003cli\u003eGeneral biochemical research reagent preparation: its good water solubility in the dihydrochloride form makes it convenient to dissolve into aqueous working solutions for biochemical experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 536-46-9\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Antibodies \u0026gt; Antibody Supplementary Products\u003c\/li\u003e\n\u003cli\u003eGrade \/ designation: for Biochemical Research\u003c\/li\u003e\n\u003cli\u003ePhysical form: solid powder (dihydrochloride salt)\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in standard TCI catalogue pack sizes; please confirm the required size when ordering\u003c\/li\u003e\n\u003cli\u003eStorage: keep in a tightly closed container, protected from air and light\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the reagent in a tightly closed container, protected from air and light, and keep it in a cool, dry place away from oxidising agents. Amber glass bottles or the original supplier container are suitable, and the container should be reclosed promptly after each use because phenylenediamine derivatives darken on exposure to air. Weigh the powder in a well-ventilated area or fume hood, and prepare aqueous solutions fresh where the method allows. Wear gloves, safety glasses and a laboratory coat during handling, avoid contact with skin and eyes and avoid inhaling dust, and dispose of residues and contaminated materials according to the chemical waste procedures of your laboratory.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"1g","offer_id":48086646161626,"sku":"TCI2510D393124365","price":536000.0,"currency_code":"IDR","in_stock":true},{"title":"5g","offer_id":48086646194394,"sku":"TCI2510D393124366","price":1125000.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510D3931.jpg?v=1769141930"},{"product_id":"tci2510d400524464","title":"TCI D4005 333338-18-4 Disodium 4-Nitrophenyl Phosphate Hexahydrate [for Biochemical Research]","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eDisodium 4-Nitrophenyl Phosphate Hexahydrate, commonly abbreviated as pNPP, is a chromogenic substrate that forms the backbone of many phosphatase-based enzymatic assays in biochemical laboratories. The compound acts as a phosphate group donor that is hydrolysed by alkaline phosphatase and acid phosphatase alike, releasing 4-nitrophenol, which develops a bright yellow colour under alkaline conditions. That colour change is measured spectrophotometrically, allowing enzyme activity to be quantified in a way that is simple, rapid, and free of complicated instrumentation. In everyday practice, pNPP serves as the bridge between an enzyme reaction that cannot be seen and an optical signal that is easy to read.\u003c\/p\u003e\n\u003cp\u003eThe characteristics that make this material such a widely chosen reagent are its good solubility in water and aqueous buffers, its stability as a hydrated disodium salt, and its low background absorbance before the reaction proceeds, so that the signal-to-noise ratio is preserved. The hexahydrate form makes weighing and dissolution straightforward at small scale, while the hydrolysis product carries a strong absorption coefficient in the visible wavelength region. The grade offered here is intended for biochemical research, matching the demands of work where reaction responses must be consistent and reproducible from one run to the next.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, pNPP is routinely used across university biochemistry teaching and research labs, clinical and diagnostic development settings, and enzyme-focused work in food, agricultural, and environmental laboratories. It appears wherever phosphatase activity has to be measured with a plate reader or a conventional spectrophotometer, and it is equally common as the detection substrate in alkaline phosphatase-conjugated immunoassay workflows, where it supports antibody-based detection alongside other antibody supplementary products.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAlkaline phosphatase activity assays — pNPP is hydrolysed efficiently by the enzyme and yields a bright yellow product under alkaline conditions, so activity can be quantified directly by absorbance.\u003c\/li\u003e\n\u003cli\u003eAcid phosphatase activity assays — the same substrate serves acid phosphatase reactions, letting a single reagent cover both enzyme classes and simplifying reagent inventory in a working biochemistry laboratory.\u003c\/li\u003e\n\u003cli\u003eELISA and immunoassay detection — as a chromogenic substrate for alkaline phosphatase-conjugated antibodies, it converts specific binding events into a readable optical signal in microplate formats.\u003c\/li\u003e\n\u003cli\u003eEnzyme kinetics and inhibitor screening — its low background absorbance and strong product absorption give a clean, linear optical readout suitable for rate measurements and inhibition studies.\u003c\/li\u003e\n\u003cli\u003eBiochemistry teaching and method development — good aqueous solubility and easy small-scale weighing in the hexahydrate form make it practical for student practicals and for validating new assay protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eProduct code: D4005\u003c\/li\u003e\n\u003cli\u003eCAS Number: 333338-18-4\u003c\/li\u003e\n\u003cli\u003eCategory: Life Science \u0026gt; Antibodies \u0026gt; Antibody Supplementary Products\u003c\/li\u003e\n\u003cli\u003ePhysical form: hydrated disodium salt (hexahydrate), water-soluble\u003c\/li\u003e\n\u003cli\u003eGrade: for biochemical research\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in standard TCI catalogue pack sizes — please confirm the size required when ordering\u003c\/li\u003e\n\u003cli\u003eStorage: store as directed on the product label; keep containers tightly closed and protected from light and moisture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the material in its original tightly closed container in a cool, dry place, protected from light and moisture, since the hydrated salt is hygroscopic in character and its assay performance depends on an intact, low-background reagent. 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