{"product_id":"tci2510d620427252","title":"TCI D6204 93183-36-9 Diisopropylammonium Tetrazol-1-ide","description":"\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eTCI D6204 Diisopropylammonium Tetrazol-1-ide is a salt formed from the tetrazolide anion and the diisopropylammonium cation, and it is widely recognised as an activating reagent in nucleic acid synthesis. In the preparation of oligonucleotides by the phosphoramidite method, every addition of a single nucleotide base requires an activator that protonates the diisopropylamino group on the phosphoramidite, converting that group into a good leaving group that can be displaced by the hydroxyl group of the growing chain. Without a suitable activator, this coupling step proceeds slowly and incompletely.\u003c\/p\u003e\n\u003cp\u003eThe property that makes this reagent such a frequent choice is the balance of its acidity. An activator must be acidic enough to protonate the phosphoramidite, yet it must not be so acidic that it causes premature loss of the dimethoxytrityl protecting group, which would lead to the insertion of incorrect bases. The ammonium salt form also confers good solubility in acetonitrile, the standard solvent for automated synthesis, so that activator solutions do not readily precipitate and block the fluid lines of the synthesiser. As a solid salt, the reagent is likewise easier to handle and weigh out than the more sensitive free tetrazole.\u003c\/p\u003e\n\u003cp\u003eIn Indonesian laboratories, this reagent belongs to the chemical biology and nucleic acid chemistry workstream, where it supports both automated and manual oligonucleotide assembly. It is typically found in university molecular biology facilities, research institutes, and laboratories that prepare custom primers, probes, and modified oligonucleotide sequences. Because coupling efficiency determines the yield of every full-length product, groups working on nucleic acid synthesis generally treat the activator as a core consumable rather than an occasional purchase.\u003c\/p\u003e\n\u003ch3\u003eLaboratory Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSolid-phase oligonucleotide synthesis: the reagent protonates the diisopropylamino group of each incoming phosphoramidite so that the coupling step to the growing chain proceeds rapidly and to completion.\u003c\/li\u003e\n\u003cli\u003eAutomated synthesiser operation: its good solubility in acetonitrile keeps activator solutions homogeneous, so the delivery lines and valves of the instrument are not blocked by precipitated solid during extended runs.\u003c\/li\u003e\n\u003cli\u003ePrimer and probe preparation: laboratories assembling custom DNA sequences rely on the reagent to maintain per-cycle coupling efficiency, since every incomplete addition reduces the yield of full-length product.\u003c\/li\u003e\n\u003cli\u003eModified oligonucleotide chemistry: the balanced acidity allows activation of phosphoramidite building blocks without prematurely removing the dimethoxytrityl protecting group and causing incorrect base insertion.\u003c\/li\u003e\n\u003cli\u003eManual and small-scale nucleic acid coupling: as a stable solid salt it is easier to weigh, dissolve, and dispense than free tetrazole, which suits benchtop work and method development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBrand: TCI\u003c\/li\u003e\n\u003cli\u003eCAS number: 93183-36-9\u003c\/li\u003e\n\u003cli\u003eCategory: Chemistry \u0026gt; Chemical Biology \u0026gt; Nucleic Acid Chemistry\u003c\/li\u003e\n\u003cli\u003ePhysical form: solid salt of the tetrazolide anion and the diisopropylammonium cation\u003c\/li\u003e\n\u003cli\u003ePack sizes: available in the catalogue pack sizes supplied by TCI for this item\u003c\/li\u003e\n\u003cli\u003eStorage: keep in the tightly closed original container, protected from moisture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHandling and Storage\u003c\/h3\u003e\n\u003cp\u003eStore the reagent in its tightly closed original container in a cool, dry place, away from moisture and from incompatible materials. Because the material is used to prepare solutions in anhydrous acetonitrile, containers and glassware should be clean and dry before weighing or dissolution, and the container should not be left open longer than necessary. Handle the solid in a fume hood using gloves, safety glasses, and a laboratory coat, and avoid generating or inhaling dust. Prepared activator solutions should be kept sealed and clearly labelled. Consult the manufacturer's safety data sheet before use, and follow local institutional rules for waste collection and disposal.\u003c\/p\u003e","brand":"TCI","offers":[{"title":"5g","offer_id":48086795747546,"sku":"TCI2510D620427252","price":2411850.0,"currency_code":"IDR","in_stock":true},{"title":"25g","offer_id":48086795780314,"sku":"TCI2510D620427253","price":8162700.0,"currency_code":"IDR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/7156\/8602\/files\/TCI2510D6204.jpg?v=1769142171","url":"https:\/\/amiscientific.com\/en\/products\/tci2510d620427252","provider":"AMI Scientific","version":"1.0","type":"link"}