DL-Dithiothreitol (DTT) - A Relatively Stable Reagent for Electrophoresis Studies

DL-Dithiothreitol (DTT) is a standard reagent for reducing disulfide bonds in proteins and stabilizing sulfhydryl groups. This reagent is often used to replace 2-mercaptoethanol due to its high effectiveness and non-pungent odor.

DTT is characterized by its tendency to be more stable than other reducing agents, but stock solutions must be properly stored to prevent degradation. Its neutral pH makes it suitable for use in electrophoresis studies.

DL-Dithiothreitol TCI D1071 Specifications

DL-Dithiothreitol TCI D1071 is often known as Cleland's Reagent and DL-1,4-Dimercapto-2,3-butanediol.

Product Information

• Product Name: DL-Dithiothreitol

• Catalog Number: D1071

• CAS Number: 3483-12-3

• Molecular Formula: C4H10O2S2

• Molecular Weight: 154.24 g/mol

Specifications

• Purity (Iodometric Titration): Min. 98.0%

• Purity (HPLC): Min. 97.0 area%

• Physical Form: Powder or crystals, white to almost white

• Melting Point: 41.0 to 45.0 °C

• Solubility in Water: Soluble (yields a nearly transparent solution)

• Other Solubility: Soluble in methanol and dichloromethane

DL-Dithiothreitol Applications

DL-Dithiothreitol can be used in protein purification processes. It performs reduction by breaking disulfide bonds between protein molecules. The addition of DTT to sample buffer denatures proteins by breaking cross-links, allowing protein molecules to move freely according to their molecular weight.

The protein purification process with DTT is useful for preventing the formation of protein aggregates. If protein purification undergoes aggregation, the protein will precipitate, leading to the loss of the desired target protein.

One use of DTT in research is to improve the accuracy of pathogen identification. In microbiological research, DTT is used to release microorganisms from biofilms on medical implants without killing the microbial bacteria.

DL-Dithiothreitol Safety Classification

DTT is classified under GHS Category 4 for acute toxicity, GHS Category 2 for skin irritation, and Category 2A for serious eye irritation. This compound has a pungent and unpleasant sulfur odor.

When using it for research, wear face protection and gloves to prevent skin contact. Also, wear closed clothing and ensure good air circulation to avoid inhaling vapors.

DTT should be stored in a tightly sealed container. Store at -20°C under nitrogen gas to maintain long-term stability.

DL-Dithiothreitol Available at AMI Scientific

AMI Scientific is a chemical distributor for academic and industrial research needs, providing DL-Dithiothreitol TCI D1071. Together with TCI Japan, we offer a distribution system for goods delivery within 2 weeks. This speed makes your research process more efficient, quicker to complete, and helps achieve publication targets. The faster it arrives, the faster you get promoted.

Accelerate your research cycle starting now. Contact AMI Scientific for consultation and ordering of DL-Dithiothreitol TCI D1071 via this link.