3alpha-Hydroxy-7-oxo-5beta-cholanic Acid TCI H0869

TCI H0869 3alpha-Hydroxy-7-oxo-5beta-cholanic Acid is a semisynthetic bile acid belonging to the steroid class, carrying a hydroxyl group at the 3-alpha position and a ketone group at position seven of the cholanic skeleton. The compound is widely known among researchers as an oxidized bile acid derivative and serves as an important reference material in studies of bile acid metabolism. In the laboratory, this material is used as a standard, as an enzyme substrate, and as a synthetic intermediate for obtaining other bile acid derivatives with differing hydroxyl configurations.

The property that makes this compound a preferred choice is the presence of the keto group at position seven, which is prochiral and can therefore be stereoselectively reduced into a hydroxyl group with either alpha or beta orientation depending on the reagent or enzyme used. This characteristic makes the compound a popular test substrate in biocatalysis research employing hydroxysteroid dehydrogenase enzymes. The curved 5-beta steroid skeleton gives it the amphiphilic character typical of bile acids, with a distinct hydrophobic face and a hydrophilic face, which is important in studies of micelle formation and interactions with biological membranes.

In Indonesian laboratories, this material is typically handled in academic research groups, pharmaceutical and biochemistry laboratories, and analytical facilities that work on bile acid metabolism and steroid chemistry. It is commonly used in small quantities for method development, preparation of reference solutions, and enzymatic conversion experiments, where consistent identity of the steroid skeleton is more important than large working volumes.

• Bile acid metabolism studies: the compound serves as a recognized oxidized bile acid derivative, allowing researchers to trace metabolic conversion pathways and compare intermediates against a defined reference material.
• Enzymatic biocatalysis research: the prochiral keto group at position seven can be stereoselectively reduced by hydroxysteroid dehydrogenase enzymes, making it a practical test substrate for evaluating enzyme selectivity.
• Synthetic intermediate preparation: laboratories use this material as a starting point for building other bile acid derivatives whose hydroxyl groups differ in orientation at the seven position.
• Analytical standard and reference solution preparation: the defined steroid structure lets analysts prepare calibration and identification solutions for bile acid work with consistent chemical identity.
• Micelle and membrane interaction studies: the amphiphilic 5-beta cholanic skeleton, with separate hydrophobic and hydrophilic faces, supports investigations of aggregation behaviour and interaction with biological membranes.

• Chemical class: semisynthetic bile acid of the steroid group, 5-beta cholanic acid skeleton

Store the container tightly closed in a cool, dry place away from direct sunlight, moisture, and sources of heat or ignition, following the storage conditions stated on the manufacturer label. Keep the material in its original container or in a clean, chemically compatible, well-sealed glass container, clearly labelled with the substance name and CAS number. Handle inside a fume hood or in a well-ventilated area, using a laboratory coat, gloves, and safety goggles. Avoid generating dust when weighing the solid, and return the container to storage promptly after use. Consult the safety data sheet before first handling and dispose of residues in accordance with laboratory chemical waste procedures.

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