5-Methylcytidine TCI M1931

TCI M1931 5-Methylcytidine is a modified nucleoside built from a 5-methylcytosine base attached to a ribose sugar. The compound is an analogue of cytidine carrying an additional methyl group at position five of the pyrimidine ring, a modification found naturally in the RNA of a wide range of organisms. In molecular biology and nucleic acid chemistry laboratories, this material serves as a reference substance and as a synthetic starting material, allowing researchers to study the role of RNA methylation, including its influence on structural stability, translation, and recognition by particular nucleic acid binding proteins inside the cell.

The properties that make this compound important are the clearly defined position of its methyl group and its status as a naturally occurring epigenetic mark. Methylation at position five does not alter the ability of the base to form Watson–Crick pairing with guanine, but it does change the steric and electronic character of the major groove, thereby affecting interactions with enzymes and reader proteins. As a free nucleoside, the molecule carries hydroxyl groups on the ribose that can be selectively protected and phosphorylated, which makes it a practical starting point for preparing phosphoramidites and other derivatives required in oligonucleotide work.

In Indonesian laboratories, this material is typically used in university research groups, biotechnology institutes, and analytical service units working on nucleic acid chemistry and epigenetics. It is generally handled in small quantities on the analytical scale, weighed out for the preparation of reference solutions or for use as a building block in multi-step synthesis. Researchers ordinarily incorporate it into method development and validation work where a well-characterised modified nucleoside standard is required.

• Analytical reference standard for RNA modification studies, where a defined 5-methylated nucleoside is needed to identify and confirm the corresponding signal obtained from digested RNA samples.
• Starting material for phosphoramidite synthesis, because the free ribose hydroxyl groups can be selectively protected and phosphorylated to build modified oligonucleotides for downstream experimental work.
• Epigenetics research on RNA methylation, since the compound represents a naturally occurring mark and lets researchers probe how methylation influences transcript stability and cellular processing.
• Enzyme and binding protein studies, as the methyl group alters major groove steric and electronic properties without disturbing Watson–Crick pairing, making recognition effects possible to isolate experimentally.
• Method development for nucleoside analysis, providing a consistent modified nucleoside against which chromatographic and spectrometric procedures can be established, compared, and validated by laboratory staff.

• Product code: M1931

Store this nucleoside in its original tightly closed container, kept in a cool, dry place away from direct sunlight, moisture, and sources of heat, and follow the storage temperature stated by the manufacturer on the product label. Use clean, dry spatulas and glassware when weighing to avoid contamination of the remaining material, and reseal the container promptly after each use. Handle the substance in a well-ventilated area or fume hood, wearing a laboratory coat, gloves, and safety glasses. Consult the safety data sheet before use, and dispose of residues and contaminated consumables according to institutional chemical waste procedures.

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