2'-O-(2-Methoxyethyl)adenosine TCI M3942

TCI's 2'-O-(2-Methoxyethyl)adenosine is a modified adenosine nucleoside carrying a 2-methoxyethyl group at the two-prime position of the ribose sugar. This modification, commonly abbreviated as MOE among oligonucleotide researchers, ranks among the most important chemical changes in the development of nucleic acid-based therapeutics. In the laboratory, the compound serves as a starting material for the preparation of phosphoramidite monomers and as a reference material in the analysis of modified oligonucleotides. Its availability allows researchers to design oligonucleotide strands that are considerably more robust than natural strands without altering their ability to recognise complementary base sequences.

The advantage that makes this nucleoside a preferred choice lies in the influence of the methoxyethyl group on strand behaviour. The substituent at the two-prime position drives the sugar ring toward a conformation favourable to duplex formation, so affinity for the target RNA strand increases and the melting temperature of the duplex rises. At the same time, the group obstructs nuclease enzymes from reaching the neighbouring phosphodiester linkages, so the oligonucleotide survives far longer in biological fluids. The methoxyethyl chain thus delivers two benefits at once — stronger binding and greater resistance to degradation — while leaving base-pairing specificity intact.

In Indonesian laboratories, this nucleoside is typically used by university and institutional research groups working on antisense oligonucleotides, RNA chemistry, and nucleic acid analysis. It is handled in small quantities on the analytical and preparative scale, most often within organic synthesis or biochemistry laboratories equipped for moisture-sensitive work. Research teams also use it as a comparative standard when characterising modified oligonucleotide products by chromatographic and spectroscopic methods.

• Phosphoramidite monomer synthesis: the nucleoside provides the protected building block needed to introduce MOE residues into oligonucleotide chains during solid-phase assembly.
• Antisense oligonucleotide research: the two-prime modification raises target RNA affinity and nuclease resistance, making it central to studies on antisense strand design and stability.
• Reference material for oligonucleotide analysis: it serves as a comparison standard when identifying and characterising modified nucleoside components in synthesised oligonucleotide samples.
• Duplex stability studies: researchers use it to examine how the methoxyethyl substituent shifts sugar conformation and raises the melting temperature of oligonucleotide duplexes.
• Nuclease stability evaluation: because the group blocks enzyme access to nearby phosphodiester bonds, it supports experiments measuring oligonucleotide survival in biological fluids.

• Catalogue number: M3942

Store the container tightly closed in a cool, dry place, following the storage conditions stated by the manufacturer on the product label and safety data sheet. Keep the material in its original container or in a tightly sealed amber glass vial, protected from moisture and direct light, since modified nucleosides are generally sensitive to humidity. Handle the compound in a well-ventilated area or fume hood, wearing gloves, safety glasses, and a laboratory coat. Use clean, dry spatulas when weighing, and allow cold containers to reach room temperature before opening to prevent condensation. Dispose of residues in accordance with institutional chemical waste procedures.

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