Bis(3-sulfo-N-succinimidyl) Glutarate Disodium Salt TCI B5778

Name: TCI B5778 881415-72-1 Bis(3-sulfo-N-succinimidyl) Glutarate Disodium Salt
Brand: TCI
SKU: TCI2510B577812518
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Berat 50mg 250mg

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Description

Bis(3-sulfo-N-succinimidyl) glutarate disodium salt is a homobifunctional crosslinking reagent belonging to the N-hydroxysuccinimide (NHS ester) group of compounds. Being homobifunctional means the molecule carries two identical reactive groups at both ends, and these NHS ester groups react specifically with primary amine (–NH₂) groups found on proteins, peptides, and other biomolecules, forming stable and durable amide bonds. In the laboratory, this reagent plays an important role in protein–protein conjugation, the attachment of proteins to solid surfaces, and the preparation of bioconjugates used in a wide range of biochemical and immunochemical assays. Its ability to link biomolecules covalently under mild conditions makes it a versatile tool for researchers working on assay development and biomolecular engineering.

The main advantage of this compound lies in its hydrophilic character, which comes from the sulfonate groups in its structure. These sulfo groups make the compound fully water-soluble, so it can be used directly in aqueous reaction systems without requiring additional organic solvents. Because no organic cosolvent is needed, the risk of organic solvents disturbing protein stability is eliminated, which is especially valuable when working with sensitive enzymes, antibodies, and other labile biomolecules. The reagent reacts rapidly with primary amines at a neutral to slightly basic pH range, allowing efficient conjugation under conditions that are generally compatible with most biological samples and that help preserve the native structure and activity of the proteins being crosslinked.

In Indonesian laboratories, this reagent is commonly used in bioconjugation workflows such as protein–protein crosslinking, antibody labeling, enzyme immobilization, and the preparation of conjugates for immunodiagnostic applications. It is particularly convenient for biochemistry and immunochemistry facilities that prefer to avoid handling organic solvents, since the reagent can be added directly to aqueous buffer systems. Academic institutions and research centers across Indonesia use it to build stable, reproducible conjugates for routine analyses as well as for more advanced studies in assay development, biosensor research, and molecular diagnostics, where reliable and gentle crosslinking chemistry is essential for obtaining consistent results.

Laboratory Applications

Protein–protein conjugation: The two identical NHS ester ends react with primary amines on separate protein molecules, forming stable amide linkages that covalently join the proteins together in purely aqueous buffer systems.
Protein immobilization on solid surfaces: The water-soluble sulfo groups allow the reagent to couple proteins onto amine-functionalized surfaces such as beads, microtiter plates, and membranes under mild, buffer-compatible conditions.
Antibody–enzyme conjugate preparation: Its fast reaction with primary amines at neutral to slightly basic pH produces active immunodiagnostic conjugates while minimizing any damage to antibody and enzyme function.
Bioconjugate synthesis for immunoassays: The reagent forms stable amide bonds with target biomolecules under gentle conditions, making it suitable for building well-defined conjugates used in ELISA and other immunochemical tests.
Crosslinking in soluble protein systems: Because no organic cosolvent is required, the reagent can be added directly to buffered protein solutions, preserving protein stability throughout the conjugation reaction.

Specifications

Brand: TCI
Product code: B5778
CAS number: 881415-72-1
Category: TCI
Chemical name: Bis(3-sulfo-N-succinimidyl) glutarate disodium salt
Description: Homobifunctional NHS ester crosslinking reagent with two identical reactive groups; fully water-soluble due to its sulfonate groups

Handling and Storage

Store the reagent in its original, tightly sealed container in a cool, dry place, protected from moisture and direct light, since NHS ester groups are sensitive to hydrolysis. Keep the container tightly closed after each use to minimize exposure to atmospheric humidity. When handling the material, wear appropriate personal protective equipment, including laboratory gloves and safety goggles, and work in a well-ventilated area. Avoid skin and eye contact, do not inhale any dust that may form, and wash hands thoroughly after handling. Follow standard laboratory safety procedures, keep the material away from incompatible substances, and always refer to the product label and safety documentation supplied by the manufacturer before use.