Di(N-succinimidyl) Pimelate TCI D5872

Name: TCI D5872 74648-14-9 Di(N-succinimidyl) Pimelate
Brand: TCI
SKU: TCI2510D587226862
Price: 2155650.0 IDR
Availability: InStock

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Berat 250mg 1g

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Description

TCI D5872 Di(N-succinimidyl) Pimelate is a homobifunctional crosslinking reagent built around a seven-carbon pimelic acid chain terminated at both ends with N-hydroxysuccinimide (NHS) ester groups. Both active ends are designed to react with primary amines, such as the lysine side chains of proteins or the free amino terminus of a peptide, forming stable amide bonds. Reagents of this kind are essential tools in chemical biology for joining two biological molecules, mapping the spatial proximity between proteins, and immobilising biomolecules onto solid surfaces in research laboratories.

The principal advantage of this reagent lies in the controlled reactivity of its NHS esters combined with a clearly defined spacer length. NHS esters react rapidly with free amines under near-neutral to slightly alkaline conditions, releasing N-hydroxysuccinimide as a by-product that is easily separated from the reaction mixture. The seven-carbon pimelate bridge provides a fixed distance between the two crosslinking points, so that crosslinking experiments can be interpreted quantitatively in structural studies. Its homobifunctional nature keeps the procedure simple, because both ends share the same selectivity toward primary amine groups.

In Indonesian laboratories, this reagent is typically used in university and institutional research groups working on protein chemistry, proteomics, and biomolecule immobilisation. It is handled in small-scale aqueous or organic reaction systems, usually as part of protein conjugation, affinity support preparation, or structural proximity work. Because it is supplied under the TCI brand, it fits laboratories that require consistent, reproducible reagent quality for method development and for teaching advanced bioconjugation techniques.

Laboratory Applications

Protein–protein crosslinking: the two identical NHS ester ends react with lysine residues on separate proteins, locking interacting partners together as stable amide-linked complexes for downstream analysis.
Spatial proximity mapping: the fixed seven-carbon pimelate bridge defines a known distance between crosslinked sites, allowing researchers to draw quantitative structural conclusions about protein architecture and contact regions.
Peptide conjugation work: free amino termini of peptides are readily coupled through the NHS ester chemistry, letting laboratories build defined peptide–peptide or peptide–protein constructs reproducibly.
Immobilisation of biomolecules on solid supports: amine-functionalised surfaces and resins can be linked to proteins or peptides, producing stable affinity materials for binding and separation experiments.
Bioconjugate preparation in chemical biology: the homobifunctional design simplifies protocol development, since a single reagent and one reaction condition handle both coupling steps toward primary amines.

Specifications

Brand: TCI
CAS number: 74648-14-9
Category: Chemistry > Chemical Biology > Linkers and Crosslinkers
Product code: D5872
Pack sizes: available in standard TCI research-scale packaging; please confirm the required size when ordering
Storage note: keep in a cool, dry place, tightly closed and protected from moisture

Handling and Storage

Store the reagent in a cool, dry location in its original tightly closed container, protected from moisture, since NHS esters are sensitive to hydrolysis. Keep the container sealed when not in use and allow it to reach room temperature before opening to limit condensation. Weigh and transfer the material in a well-ventilated area or fume hood, using clean, dry glassware or plastic labware and anhydrous solvent where a stock solution is required. Wear a laboratory coat, safety glasses, and suitable gloves during handling, avoid contact with skin, eyes, and inhalation of dust, and wash hands after use. Prepare working solutions fresh before an experiment and dispose of residues and contaminated consumables in accordance with local chemical waste procedures. Always consult the manufacturer's safety data sheet before first use.