3,3'-Diaminobenzidine [for Biochemical Research] TCI D3756

Name: TCI D3756 91-95-2 3,3'-Diaminobenzidine [for Biochemical Research]
Brand: TCI
SKU: TCI2510D375624119
Price: 827400.0 IDR
Availability: InStock

Rp 827.400,00

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Berat 1g 5g

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Description

3,3'-Diaminobenzidine, commonly abbreviated as DAB, is a tetra-amino aromatic compound prepared for biochemical research purposes. Its molecule consists of two directly linked benzene rings, each carrying two amino groups, which provides four nucleophilic centres together with a readily oxidised pi-electron system. Its principal role in the laboratory is as a chromogenic substrate in reactions catalysed by peroxidase enzymes, where DAB is oxidised and precipitates as an insoluble brown product exactly at the site of the enzyme. In addition, this tetra-amino framework is also used as a monomer and building block in heterocyclic synthesis.

The characteristics that make DAB such a widely chosen reagent are the stability, contrast, and strong adherence of its oxidation precipitate to tissues or membranes. The brown colour that forms is insoluble in both water and alcohol, so preparations can be dehydrated, cleared, and mounted in a permanent medium without any loss of signal. It is precisely this property that allows stained preparations to be archived and re-examined years later. As a free base, the compound is generally dissolved first in a suitable solvent before use in the working system.

In Indonesian laboratories, this material is typically handled in biochemistry, molecular biology, histology, and pathology work, where peroxidase-based detection is a routine part of the workflow. It is also drawn on by organic and materials synthesis groups that require a non-heterocyclic building block with multiple amino centres. Supplied as a TCI catalogue item under the Chemistry > Building Blocks > Non-Heterocyclic Building Blocks category, it is ordered by university research units, hospital laboratories, and industrial R&D facilities alike.

Laboratory Applications

Immunohistochemistry (IHC) detection: DAB oxidised by horseradish peroxidase deposits an insoluble brown precipitate directly at the antigen site, giving sharp, high-contrast localisation on tissue sections.
Western blot membrane development: the precipitate adheres firmly to the blotting membrane and does not wash away, producing permanent visible bands that require no imaging equipment to read.
Immunocytochemistry on cultured cells: because the coloured product is insoluble in water and alcohol, stained cell preparations survive dehydration and clearing without any loss of signal intensity.
Enzyme histochemistry and peroxidase activity mapping: the reaction proceeds precisely where the enzyme is located, so endogenous or introduced peroxidase activity can be mapped with good spatial fidelity.
Heterocyclic and polymer synthesis: the four amino groups on a directly linked biphenyl framework make this a versatile monomer and building block for condensation into heterocyclic ring systems.

Specifications

Brand: TCI (Tokyo Chemical Industry), catalogue number D3756
CAS number: 91-95-2
Category: Chemistry > Building Blocks > Non-Heterocyclic Building Blocks
Grade: [for Biochemical Research]
Chemical form: free base of the tetra-amino aromatic compound, dissolved in a suitable solvent before use
Pack sizes: available in the standard TCI research pack sizes; storage conditions follow the manufacturer's label

Handling and Storage

Store the container tightly closed in a cool, dry place away from direct sunlight, heat sources, and strong oxidising agents, following the storage conditions stated on the manufacturer's label. Amber glass or the original supplier container is preferred, since the compound is readily oxidised and light exposure should be minimised. Handle weighing and dissolution inside a fume hood, and wear a laboratory coat, nitrile gloves, and eye protection throughout. Avoid raising dust, and do not inhale or allow contact with skin. Prepare working solutions fresh where possible and keep them protected from light. Collect residues and used solutions as designated chemical waste for disposal in accordance with applicable institutional and local regulations.