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Description
TCI D5701 DMT-dC(bz) Phosphoramidite is a phosphoramidite monomer used for the chemical synthesis of DNA oligonucleotides. It is a deoxycytidine derivative in which the 5'-hydroxyl group is protected by a dimethoxytrityl group, the exocyclic amine of the base is protected by a benzoyl group, and the 3'-position carries a reactive phosphoramidite group. In the laboratory, this material serves as one of the four basic monomers that DNA synthesizers use to assemble a nucleotide chain one residue at a time on a solid support. Each synthesis cycle comprises removal of the trityl protecting group, coupling of the monomer, capping of unreacted groups, and oxidation of the phosphite linkage to a phosphate.
The characteristics that determine the quality of this monomer are its functional group protection pattern and its chemical cleanliness. The dimethoxytrityl group can be removed selectively with a weak acid and releases a characteristic colour that instruments use to monitor the coupling efficiency of each cycle, giving operators a direct readout of how the synthesis is progressing. The benzoyl group protects the exocyclic amine of cytosine throughout the synthesis and is then removed during the final base-mediated deprotection step. Low water content and low impurity levels are critically important, because phosphoramidites are highly sensitive to moisture; even a small amount of water can reduce coupling performance across the assembly.
In Indonesian laboratories, this monomer is typically used in university, research institute, and biotechnology settings where DNA oligonucleotides are prepared in-house rather than ordered as finished sequences. It is handled as a consumable on automated synthesizers, dissolved in dry solvent and installed on the instrument as one of the four standard base positions. Groups working on nucleic acid chemistry, molecular biology reagent preparation, and method development draw on it for routine and custom oligonucleotide assembly.
Laboratory Applications
Automated DNA oligonucleotide synthesis: supplies the protected cytosine monomer required at every C position during solid-support chain assembly on a synthesizer.
Primer and probe preparation: allows laboratories to build their own sequences in-house, with the trityl colour release confirming that each coupling step has proceeded efficiently.
Nucleic acid chemistry research and method development: serves as a well-defined, standard-pattern building block for studying coupling, capping, and oxidation steps in phosphoramidite chemistry.
Modified and custom oligonucleotide construction: functions as the standard dC(bz) component alongside other monomers when sequences are designed with non-routine lengths or compositions.
Teaching and training in solid-phase synthesis: provides a conventional protection scheme suitable for demonstrating the full deprotection, coupling, capping, and oxidation cycle to students and new operators.
Specifications
Brand: TCI
CAS number: 102212-98-6
Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
Product code: D5701
Pack sizes: available in the manufacturer's standard pack sizes for this catalogue item
Storage: moisture-sensitive material; store under the manufacturer's recommended conditions
Handling and Storage
Because phosphoramidites are highly sensitive to moisture, this material should be kept tightly closed and protected from humidity at all times, following the storage conditions stated by the manufacturer. Use containers that maintain a dry, sealed environment, and allow the container to reach ambient temperature before opening so that condensation does not form on the contents. Dissolve the monomer in dry solvent immediately before use on the synthesizer, and handle it in a low-humidity environment where possible. Standard laboratory precautions apply: work in a fume hood, wear gloves, safety glasses, and a laboratory coat, avoid contact with skin and eyes, and consult the manufacturer's safety data sheet before handling and for waste disposal.
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