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Description
TCI D5702 DMT-dT Phosphoramidite is a thymidine phosphoramidite monomer used for the chemical synthesis of DNA oligonucleotides. Its 5' hydroxyl group is protected by a dimethoxytrityl (DMT) group, while the 3' position carries a phosphoramidite function that is reactive toward coupling. Unlike the adenine, cytosine, and guanine monomers, thymine has no exocyclic amine, so this monomer requires no additional protecting group on its base. In the laboratory it serves as one of the four core monomers delivered by an automated DNA synthesiser, building an oligonucleotide strand step by step on a solid support through the repeating cycle of deprotection, coupling, capping, and oxidation.
The most notable characteristics of this material are the simplicity of its protection pattern and the reliability of its coupling reaction. Because the base itself does not need to be protected, thymidine monomers generally show clean and efficient synthesis behaviour, and they are frequently used as a benchmark monomer when researchers evaluate instrument performance or reagent quality. The dimethoxytrityl group also allows the efficiency of each individual cycle to be monitored through colorimetric measurement of the trityl cation released during detritylation. As with all phosphoramidites, quality is determined by low water content and freedom from contaminants that would interfere with the coupling step.
In Indonesian laboratories, this monomer is used in academic and research facilities that operate their own oligonucleotide synthesis capability, including molecular biology, biotechnology, and nucleic acid chemistry groups. It is typically loaded onto an automated synthesiser together with the other three standard monomers to prepare primers, probes, and custom DNA sequences for downstream work. Because its behaviour is predictable, it is also a practical choice for training new operators and for routine verification of synthesiser and reagent performance.
Laboratory Applications
- Automated DNA oligonucleotide synthesis — supplies the thymidine residue during each coupling cycle on solid support, making it one of the four indispensable monomers for assembling any DNA sequence.
- PCR primer preparation — allows laboratories to synthesise their own custom primers in house, with the thymidine positions of the designed sequence introduced directly by this monomer.
- Hybridisation probe synthesis — supports the construction of defined DNA probes for detection and blotting work, where accurate sequence assembly at every T position is essential.
- Synthesiser and reagent performance checks — its simple protection pattern and clean coupling behaviour make it the preferred reference monomer for evaluating instrument condition and reagent quality.
- Coupling efficiency monitoring during synthesis — the released dimethoxytrityl cation gives a colorimetric signal that lets operators track stepwise yield cycle by cycle throughout a run.
Specifications
- Brand: TCI (Tokyo Chemical Industry)
- CAS Number: 98796-51-1
- Catalogue Number: D5702
- Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
- Pack sizes: available in the standard research pack sizes offered for this catalogue item; please confirm the required size when ordering
- Storage: keep in a cool, dry place under the moisture-free conditions required for phosphoramidite reagents
Handling and Storage
Store this phosphoramidite in a cool, dry location in its original tightly closed container, protected from moisture and atmospheric humidity, since water content is the principal factor that degrades coupling performance. Keep the reagent in a sealed, moisture-resistant vessel and allow the container to reach ambient temperature before opening to prevent condensation on the contents. Handle in a dry environment, ideally under an inert atmosphere, and use clean, dry glassware and anhydrous solvents when dissolving the material for use on a synthesiser. Work in a well-ventilated area or fume hood, wear gloves, safety glasses, and a laboratory coat, and consult the manufacturer's safety data sheet before handling. Avoid contact with contaminants that would interfere with the coupling step, and reseal the container immediately after each use.
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