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Description
TCI D5704 DMT-2'-O-methyl-rU Phosphoramidite is a modified uridine phosphoramidite monomer used in the chemical synthesis of RNA oligonucleotides. Its structure carries a methyl group at the 2'-O position of the ribose sugar, a dimethoxytrityl (DMT) group protecting the 5'-hydroxyl, and a reactive phosphoramidite group at the 3' position. Because the uracil base has no exocyclic amine, this monomer requires no additional base protection. In the laboratory, the material is used to place 2'-O-methyl uridine residues at selected positions within an oligonucleotide strand, allowing researchers to design synthetic RNA with greater resistance to enzymatic degradation while retaining sequence recognition capability.
The property that makes this monomer important is the combination of chemical simplicity and the benefits of sugar modification. With no base protecting group in place, coupling proceeds cleanly and predictably, while the 2'-O-methyl group blocks hydrolysis by ribonucleases and favours a sugar conformation that is advantageous for duplex stability with a target RNA. The modification also simplifies the synthesis process compared with native RNA, because there is no 2' protecting group that must be removed at the final stage. Like other phosphoramidites, the material is moisture-sensitive and is handled under conditions that keep water out of the coupling chemistry.
In Indonesian laboratories, this monomer is typically used by research groups and service facilities that assemble oligonucleotides on automated synthesisers, whether for molecular biology, biochemistry, or nucleic acid chemistry work. It is generally ordered as part of a set of phosphoramidite building blocks, dissolved in anhydrous solvent immediately before a synthesis run, and used in campaigns where modified RNA strands are needed. Its handling requirements make it best suited to facilities with dry solvent supply and controlled storage.
Laboratory Applications
Synthesis of modified RNA oligonucleotides: the monomer inserts 2'-O-methyl uridine at chosen positions during automated solid-phase assembly, giving direct control over where the sugar modification appears in the strand.
Preparation of nuclease-resistant synthetic RNA: because the 2'-O-methyl group blocks ribonuclease hydrolysis, strands built with this monomer survive enzymatic conditions far better than fully unmodified RNA sequences.
Design of duplex-stabilised RNA constructs: the 2'-O-methyl substitution favours a sugar conformation that supports stable duplex formation with a target RNA, useful when hybridisation strength matters.
Sequence-recognition studies with modified backbones: researchers can add the sugar modification while keeping base pairing intact, so recognition of the intended sequence is preserved throughout the construct.
Simplified RNA synthesis workflows: with no exocyclic amine to protect and no 2' protecting group to remove, coupling and final deprotection steps are cleaner and more predictable than with native RNA amidites.
Specifications
Brand: TCI
CAS number: 110764-79-9
Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
Pack sizes: available in the pack sizes offered by TCI for this catalogue item (D5704); please confirm the required quantity when ordering.
Physical form: solid phosphoramidite monomer, dissolved in anhydrous solvent before use on a synthesiser.
Storage note: moisture-sensitive; store as recommended by the manufacturer and keep protected from water and humid air.
Handling and Storage
This phosphoramidite is moisture-sensitive and should be kept tightly closed in its original container, protected from humid air, and stored under the cool conditions recommended by the manufacturer for this product. Allow the container to reach ambient temperature before opening to avoid condensation on the solid, and dissolve the monomer in anhydrous solvent only immediately before a synthesis run. Use dry glassware or septum-sealed vials, and handle transfers with dry syringes or under an inert atmosphere where the workflow allows. General laboratory precautions apply: work in a fume hood when handling anhydrous solvents, wear gloves, a laboratory coat, and eye protection, avoid contact with skin and eyes, and dispose of residues and solvent waste according to institutional chemical waste procedures.
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