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Description
TCI D5707 DMT-dA(Bz) Phosphoramidite is a protected nucleoside monomer that serves as a fundamental building block for the chemical synthesis of DNA oligonucleotides. The molecule carries an adenine base whose amino group is protected as a benzoyl (Bz) group, a 5'-hydroxyl group capped with dimethoxytrityl (DMT), and a reactive phosphoramidite group at the 3' position. This combination of protecting groups and reactive functionality is precisely what allows bases to be added one at a time in a controlled manner during solid-phase synthesis, so that researchers can assemble DNA sequences exactly as designed, with a direction and length entirely of their own choosing.
The main characteristics that make this monomer a preferred choice are its mutually orthogonal protection pattern and its status as a standard in nucleic acid chemistry. The DMT group can be removed selectively with a weak acid at each cycle, exposing a free hydroxyl for the following coupling step, while the benzoyl group remains intact until the final base-mediated deprotection stage. The phosphoramidite group itself is highly reactive toward azole activators, so the coupling reaction proceeds rapidly and efficiently. These properties underpin the efficiency of each individual cycle, which in turn determines the final yield of the assembled oligonucleotide.
In Indonesian laboratories, this monomer is typically used in research groups and service facilities that operate automated oligonucleotide synthesizers for molecular biology work. It fits routine preparation of custom DNA sequences where the direction, length, and base order are defined by the researcher rather than purchased ready-made, and it is equally suited to teaching and method-development settings in universities, research institutes, and applied laboratories that build their own nucleic acid chemistry capability.
Laboratory Applications
- Solid-phase DNA oligonucleotide synthesis: the 3'-phosphoramidite group couples rapidly under azole activation, enabling controlled, cycle-by-cycle addition of adenine residues on a solid support.
- Custom primer and probe preparation: the orthogonal DMT and benzoyl protection lets researchers assemble sequences of self-defined direction and length with the correct base order throughout.
- Automated synthesizer workflows: standardised protection chemistry means this monomer follows conventional detritylation, coupling, and final base deprotection steps without altering established instrument cycles.
- Nucleic acid chemistry method development: as a widely adopted standard monomer, it provides a reliable reference building block when coupling conditions or activator systems are being optimised and compared.
- Teaching and training in oligonucleotide chemistry: the clearly defined roles of the DMT, benzoyl, and phosphoramidite groups make it a practical example for demonstrating stepwise synthetic strategy.
Specifications
- Brand: TCI
- CAS number: 98796-53-3
- Catalogue reference: D5707
- Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
- Chemical class: protected nucleoside monomer bearing benzoyl-protected adenine, a 5'-DMT group, and a 3'-phosphoramidite group
- Pack sizes: available in the manufacturer's standard packaging options; please confirm the required size when ordering
- Storage: keep sealed and store according to the manufacturer's stated conditions on the product label
Handling and Storage
Store the container tightly closed in a cool, dry place, following the storage conditions stated by the manufacturer on the product label. Phosphoramidite chemistry is sensitive to moisture, so keep the material sealed in its original container, minimise exposure to atmospheric humidity, and allow cold material to reach ambient temperature before opening to avoid condensation. Handle and weigh the compound under dry conditions, preferably in a fume hood, using clean and thoroughly dried glassware and anhydrous solvents. Wear a laboratory coat, safety glasses, and suitable gloves, keep the compound away from acids, bases, and incompatible reagents, and dispose of residues and contaminated consumables in line with institutional chemical waste procedures.
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