• DMT-2'-O-methyl-rG(Ib) Phosphoramidite (CAS 150780-67-9) - TCI D5710 - AMI Scientific
1 1

TCI

CAS 150780-67-9

DMT-2'-O-methyl-rG(Ib) Phosphoramidite TCI D5710

DMT-2'-O-methyl-rG(Ib) Phosphoramidite TCI D5710
Rp 1.771.350,00
Rp 1.771.350,00
Sale Sold out
Shipping calculated at checkout.
Berat
Pembayaran hanya melalui request quotation dan dilakukan setelah tim kami menghubungi Anda. Mohon cantumkan nomor WhatsApp aktif untuk konfirmasi pesanan & pembuatan invoice. Estimasi pengiriman 4-5 minggu setelah pembayaran.

Dokumen Keamanan & Mutu

↓ Unduh SDS / MSDS (PDF resmi TCI)
CoA (Certificate of Analysis)

Spesifik per batch produksi — diterbitkan dan dikirim bersama barang sesuai nomor lot yang Anda terima.

Contoh CoA segera tersedia.

💬 Minta CoA via WhatsApp

View full details

TCI D5710 DMT-2'-O-methyl-rG(Ib) Phosphoramidite is a guanosine monomer carrying a 2'-O-methyl group, designed for the synthesis of modified RNA oligonucleotides. The guanine base is protected as the isobutyryl amide, the 5'-hydroxyl is capped with a dimethoxytrityl group, and the 3' position bears the phosphoramidite function that serves as the coupling point during solid-phase synthesis. This monomer completes the set of four 2'-O-methyl monomers, so researchers can assemble a fully modified sequence or place the modification only at selected positions, according to the oligonucleotide design strategy under development.

What makes this monomer valuable is the combination of the stability of the isobutyryl protecting group and the structural advantages of the 2'-O-methyl group. Methylation at the 2' position drives the sugar toward an RNA-like conformation, so the duplex that forms is more compact and its affinity increases, while the loss of the free 2'-hydroxyl eliminates the principal route of degradation by ribonucleases. Because there is no bulky 2' protecting group, steric hindrance at the coupling step is lower than with conventional RNA monomers, giving higher cycle efficiency — particularly at guanosine positions, which are usually the most difficult in any given sequence.

In Indonesian laboratories, this monomer is used in academic and institutional groups that operate automated oligonucleotide synthesizers for antisense, siRNA, and aptamer work, as well as in facilities that prepare modified probes and primers in house. It is typically ordered alongside the other three 2'-O-methyl monomers so that a complete modified sequence can be built in a single synthesis campaign, and drawn on selectively when only certain positions in a construct need to carry the modification.

  • Modified RNA oligonucleotide synthesis — the 3'-phosphoramidite group provides the coupling point for standard solid-phase cycles, letting the guanosine position be introduced without changing the established synthesis chemistry.
  • Antisense oligonucleotide preparation — the absence of a free 2'-hydroxyl removes the main ribonuclease degradation pathway, so sequences built with this monomer resist enzymatic breakdown better than unmodified RNA.
  • siRNA and duplex construct assembly — the RNA-like sugar conformation induced by 2'-O-methylation yields a more compact duplex with increased affinity for its complementary strand.
  • Site-specific modification of existing sequences — because the full set of four 2'-O-methyl monomers is available, the modification can be placed only at chosen positions rather than throughout the sequence.
  • Difficult guanosine-rich sequence synthesis — reduced steric hindrance compared with conventional RNA monomers raises coupling efficiency at guanosine, typically the hardest position in a sequence to incorporate cleanly.

Brand TCI
CAS number 150780-67-9
Molecular formula —
Purity —
Category Chemistry > Chemical Biology > Nucleic Acid Chemistry
Pack sizes available in the pack sizes listed for this catalogue item
Physical form —
Storage store under the conditions stated on the manufacturer's label
  • Product code: D5710

Phosphoramidite monomers are moisture-sensitive reagents and should be kept tightly closed in their original container, stored cold and dry as indicated on the manufacturer's label, and allowed to reach room temperature before opening so that condensation does not enter the vial. Handle and dispense under dry inert atmosphere wherever possible, using anhydrous solvents and dry glassware or septum-capped vials for transfer to the synthesizer. Work in a well-ventilated fume hood with gloves, safety glasses, and a laboratory coat, avoid contact with skin and eyes, keep the reagent away from water and moist air, and dispose of residues and contaminated solvents through the laboratory's chemical waste route in accordance with the safety data sheet.

—

Produk Terkait