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Description
TCI D5754, CAS 220524-71-0, is Green DND-26, a fluorescent dye built on a boron difluoride dipyrromethene framework and belonging to the dipyrromethene, or BODIPY, family. Compounds in this class are widely recognised as cell imaging probes because they produce bright green emission, and variants carrying a weakly basic amine group such as morpholine are used specifically to label acidic organelles inside living cells. In cell biology and fluorescence microscopy laboratories, this material serves as a marker dye that helps researchers observe the distribution and dynamics of organelles directly.
The properties that make dipyrromethene dyes such a frequent choice are their narrow, sharp absorption and emission bands, high fluorescence quantum yield, and relatively low sensitivity to changes in solvent polarity and the acidity of the medium. Together these characteristics produce a bright, stable signal against a low background, so the resulting images are easier to interpret. The lipophilic character of the dye allows it to cross the membranes of living cells without any permeabilisation treatment, while the weakly basic amine group promotes accumulation in low-pH compartments.
In Indonesian laboratories, this material is used in the same way as in cell biology and fluorescence microscopy work elsewhere: as a marker dye applied to living cells so that organelle distribution and behaviour can be followed under the microscope. It fits research groups and teaching laboratories that run live-cell imaging, where a dye that enters cells without permeabilisation and gives a bright, stable green signal simplifies both sample preparation and image interpretation.
Laboratory Applications
Live-cell fluorescence imaging — the dye crosses living cell membranes without permeabilisation, so cells can be labelled and observed directly without extra preparation steps that disturb them.
Acidic organelle labelling — the weakly basic amine group drives accumulation in low-pH compartments, allowing researchers to mark acidic organelles selectively within intact living cells.
Organelle distribution studies — bright green emission against a low background makes it straightforward to map where labelled organelles sit throughout the cell volume.
Organelle dynamics observation — the high fluorescence quantum yield and stable signal support following how labelled organelles move and change over the course of an experiment.
Multi-channel fluorescence microscopy — narrow, sharp absorption and emission bands help keep the green channel well separated from other dyes used alongside it in the same sample.
Pack sizes: available in the pack sizes offered by the manufacturer for this catalogue number
Storage: store as directed on the manufacturer's label and product documentation
Handling and Storage
Store the product under the conditions stated on the manufacturer's label and accompanying documentation, keeping the container tightly closed when not in use. As with other fluorescent dyes, protect the material from prolonged exposure to light and keep it in its original container or an equivalent tightly sealed, light-protected vessel. Handle in a well-ventilated area or fume hood, wearing a laboratory coat, gloves, and eye protection, and avoid contact with skin and eyes as well as inhalation of any dust. Prepare and transfer solutions using clean, dry glassware to prevent contamination, label all working solutions clearly, and consult the manufacturer's safety data sheet before use and for disposal guidance.
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