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Description
The TCI D5940 136834-22-5 DMT-2'-Fluoro-dA(Bz) Phosphoramidite is a specialized chemical compound used in nucleic acid synthesis, specifically in the construction of oligonucleotides. It plays a crucial role in chemical biology laboratories where precise modifications to DNA and RNA sequences are required. This phosphoramidite is a key reagent in solid-phase synthesis, enabling the controlled assembly of nucleotide chains with specific base modifications. Its application is essential for creating modified nucleic acids with enhanced properties for various research purposes.
This compound is preferred due to its chemical stability under controlled conditions and its ability to undergo controlled reactions during synthesis. The phosphoramidite structure allows for efficient coupling with other reagents, ensuring high yield and accuracy in oligonucleotide production. Its sensitivity to moisture and oxidation necessitates careful handling, but this also ensures that the final product maintains high purity and specificity. These properties make it ideal for applications requiring high precision and reproducibility in nucleic acid synthesis.
In Indonesian laboratories, this phosphoramidite is commonly used in biomedical research, diagnostic development, and pharmaceutical applications. Researchers in universities and research institutions rely on it for creating modified oligonucleotides used in gene therapy, molecular diagnostics, and drug discovery. Its role in advancing genetic research and therapeutic development is significant, making it a valuable tool in modern molecular biology laboratories.
Laboratory Applications
Biochemical synthesis of modified oligonucleotides for gene therapy applications due to its precise coupling properties.
Development of diagnostic tools requiring specific base modifications for enhanced detection accuracy.
Production of nucleic acid probes for molecular biology research, leveraging its controlled reactivity in synthesis.
Support in pharmaceutical research for creating therapeutic oligonucleotides with targeted modifications.
Use in genetic engineering projects where specific base substitutions are necessary for functional studies.
Specifications
Brand: TCI
CAS number: 136834-22-5
Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
Pack sizes: Not specified
Physical form: Not specified
Storage note: Requires dry, cool conditions to prevent degradation
Handling and Storage
This phosphoramidite should be stored in a dry, cool environment to maintain its chemical stability and prevent degradation. It is recommended to use airtight containers to minimize exposure to moisture and oxygen, which can lead to oxidation and reduced efficacy. Due to its sensitivity, it should be handled in a controlled laboratory setting with appropriate safety measures, such as gloves and fume hoods. Proper storage ensures the compound remains viable for use in nucleic acid synthesis. Regular monitoring of storage conditions is essential to maintain the integrity of the material during long-term use.
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