Cholecalciferol - Easily Formulated Organic Steroid

Cholecalciferol is an organic steroid compound belonging to the secosteroid family. Chemically, this compound is formed from 7-dehydrocholesterol. When exposed to ultraviolet (UV) radiation, a photochemical reaction occurs that breaks certain carbon bonds, transforming it into a vitamin D3 precursor, which then changes into cholecalciferol through thermal isomerization.

Cholecalciferol (C27H44O) has an open steroid nucleus structure. While regular steroids have four closed rings (A, B, C, and D), in this compound, the B ring is broken at the bond between carbon atoms C9 and C10 due to a photochemical reaction from UV light.

Cholecalciferol Specifications

• Chemical Name: 9,10-Secocholesta-5,7,10(19)-triene-3-ol, (3β,5Z,7E)-.

• Molecular Formula: C27H44O

• Molecular Weight: 384.64 g/mol.

• CAS Number: 67-97-0.

• Description: White to yellowish-white crystalline powder, odorless, and in the form of needles or prisms.

• Melting Point: Ranges from 83°C to 86°C.

• Solubility:

• Insoluble in water.

• Freely soluble in alcohol, chloroform, and ether.

• Soluble in vegetable oils (such as corn oil).

Cholecalciferol Handling

Handling Cholecalciferol requires precision due to its chemical properties, which are sensitive to environmental factors and can be dangerous if exposed in pure doses because it is acutely toxic.

Using PPE

Pure Cholecalciferol is toxic and can be harmful to your health. Therefore, before conducting research, it is mandatory to use full-body PPE. N100 dust masks, chemical-resistant gloves, and safety goggles are a must.

Dissolution Technique

Cholecalciferol is non-polar, so use organic solvents such as ethanol, chloroform, or methanol. Do not dissolve it in water due to different types.

Weigh the pure powder in a well-ventilated fume hood to avoid dust formation. If Cholecalciferol spills on the ground, immediately clean it with a damp cloth and dispose of it in a special chemical waste container.

Store at -20°C

After use, store the remaining test material in a freezer at -20°C. Place it in a tightly sealed container.

Advantages of Cholecalciferol

1. Cost Efficacy

Cholecalciferol has higher bioavailability than Ergocalciferol. This is evident from the lower dosage required to increase vitamin D levels in the blood. Furthermore, the volume of raw materials used is much smaller, which reduces logistics costs.

2. Easy to Formulate

The Cholecalciferol compound can be processed into various forms. Firstly, it is easily dissolved in vegetable oil for liquid drops. Then, in fine granular form, it can be used for dry tablet mixtures or powdered milk without damaging its chemical structure.

This ease of formulation is also directly proportional to better stability against heat and humidity. Once it becomes a final product, it has a more stable and consistent shelf life.

3. Global Regulation Friendly

Cholecalciferol has been recognized by global safety authorities as a GRAS (Generally Recognized as Safe) substance. In simple terms, this compound has passed BPOM (Indonesia's Food and Drug Authority) approval.

Given that Cholecalciferol has passed global safety tests, the product registration process for the international market is easy because its specification standards are accepted worldwide.

4. Soluble in Various Matrices

Cholecalciferol can be emulsified so that it dissolves in water-based products such as milk or functional beverages without changing the taste, color, or texture of the final product. This method is used by the food industry to maintain the consistency of the final product until it reaches consumers.

Applications (reagents) of Cholecalciferol in Research

Cholecalciferol is an active ingredient for various experiments. The following are some applications as active reagents.

1. Reference Standard

Cholecalciferol is used as an external reference due to its high purity. This test is used as an external standard in instrumental analysis methods.

Food, beverage, or supplement samples will be tested to measure vitamin D3 levels.

2. Cell Culture Medium

Cholecalciferol is added to culture media to study cellular responses. This test is often performed in cell biology research.

Cholecalciferol compounds are used as reagents to induce differentiation of stem cells into bone cells.

3. Pharmaceutical Formulation Research

Cholecalciferol is used as a molecule in the development of pharmaceutical technology. This compound acts as an active substance to test the effectiveness of drug delivery systems such as liposomes, lipid nanoparticles, or microemulsions.

Cholecalciferol Available at AMI Scientific

Cholecalciferol is an organic compound with high purity often used as a reference standard for food products to measure vitamin D3 levels. Together with TCI Japan, we offer a distribution system that delivers goods within 2 weeks. This speed makes your research process more efficient, faster to complete, and publication targets achieved.

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