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Description
TCI D3789 Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate [for Biochemical Research], CAS number 6381-92-6, is the disodium salt of EDTA supplied in its dihydrate form and prepared for biochemical research work. It is a versatile chelating agent that binds divalent and trivalent metal ions such as calcium, magnesium, iron, and zinc, forming stable water-soluble complexes. Because a great many enzymes and biological processes depend on metal ions, EDTA is one of the most frequently encountered reagents in molecular biology, cell culture, and chemical analysis laboratories.
Several properties make this material a preferred choice. Its hexadentate coordinating groups bind metal ions strongly, giving reliable and reproducible sequestration across a wide range of sample types. Its good solubility in water suits aqueous buffer preparation, and the disodium salt form is far more practical to work with than free-acid EDTA. EDTA stock solutions are normally prepared with pH adjustment using sodium hydroxide so that optimal solubility is reached — a routine step familiar to every molecular biology laboratory. The [for Biochemical Research] designation confirms that the grade of this material is prepared for sensitive biological work.
As a stable crystalline solid, this material is straightforward to handle, weigh, and store in typical Indonesian laboratory conditions. It is used across university research laboratories, molecular biology and cell culture facilities, hospital and clinical research units, and quality control laboratories, where EDTA solutions are prepared in-house as part of standard buffer systems. Its status as a routine, high-turnover reagent means it is normally kept as a standing stock item on the general reagent shelf rather than ordered per experiment.
Laboratory Applications
- Buffer and stock solution preparation: EDTA is a standard component of TE, TAE, and TBE buffer systems, and the water-soluble disodium salt dissolves readily with sodium hydroxide pH adjustment for reproducible stock solutions.
- Nuclease inhibition in molecular biology: by chelating the divalent metal ions that many nucleases require as cofactors, this reagent helps protect nucleic acid samples during extraction, handling, and storage.
- Cell culture reagent and supplement work: EDTA sequesters calcium and magnesium ions in aqueous media formulations, which is why it appears among reagents and supplements used in cell culture laboratories.
- Enzyme activity studies: because numerous enzymes depend on metal ion cofactors, controlled addition of this chelating agent allows researchers to examine metal dependence and to suppress unwanted metal-catalysed activity.
- Chemical and analytical determinations: the strong, stable water-soluble complexes formed with calcium, magnesium, iron, and zinc make EDTA a widely used complexing agent in routine chemical analysis procedures.
Specifications
- Brand: TCI (Tokyo Chemical Industry)
- CAS number: 6381-92-6
- Category: Life Science > Cell Culture > Reagents and Supplements [Cell Culture]
- Grade designation: for Biochemical Research
- Physical form: stable crystalline solid; soluble in water (disodium salt, dihydrate)
- Pack sizes: available in TCI catalogue pack sizes — please confirm the currently listed packaging option when ordering
- Storage: store in a tightly closed container in a cool, dry place
Handling and Storage
Store the material in its original tightly closed container in a cool, dry place, protected from moisture so that the crystalline solid remains free-flowing and the dihydrate form is preserved. Suitable containers are the supplier's original packaging or clean, dry, well-sealed glass or chemically compatible plastic bottles, clearly labelled with the substance name, CAS number, and preparation date for any solutions made in-house. Follow general laboratory practice: weigh the solid in a well-ventilated area or fume hood to avoid dust generation, wear a laboratory coat, safety glasses, and gloves, and avoid contact with skin and eyes and inhalation of dust. Use clean, dedicated spatulas to prevent cross-contamination, close the container immediately after dispensing, and keep prepared aqueous stock solutions labelled and stored according to your laboratory's standard procedures. Always consult the manufacturer's Safety Data Sheet before first use and dispose of waste in line with local regulations and institutional policy.
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