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Description
Ethyl 2,5-Dihydroxybenzoate is the ethyl ester of gentisic acid (2,5-dihydroxybenzoic acid), a phenolic compound carrying two hydroxyl groups on the benzene ring together with a single ester group. It is classified as a bioactive compound, that is, a biochemical material used in research to study the biological activity associated with a given molecular structure. In the laboratory, materials of this type serve as test compounds in activity screening, as comparison standards in instrumental analysis, and as intermediates in the synthesis of more complex phenolic derivatives. The presence of the dihydroxy substitution gives the molecule the characteristic chemistry of the aromatic hydroquinone class.
The property that makes this compound a preferred choice is its 2,5-dihydroxy substitution pattern, which confers reductive character and radical-scavenging capability, as is commonly encountered among phenolic compounds. The ethyl ester group makes the molecule more lipophilic than the free acid, so its solubility in organic solvents is better and its penetration in membrane-based test systems typically differs from that of the acid form. The combination of hydroxyl groups capable of forming hydrogen bonds with an ester group that can be hydrolysed makes this a versatile structure for researchers who need a defined phenolic scaffold to work from.
In Indonesian laboratories, a compound of this kind is normally handled within university research groups, government research institutes, and private analytical or formulation laboratories. It is typically weighed out in small quantities for dissolution in an organic solvent, then used for screening assays, for preparing comparison solutions on instruments, or as a starting material in derivative synthesis. Because it belongs to the bioactive compound group, it is usually ordered per research protocol rather than as a bulk routine consumable.
Laboratory Applications
- Bioactivity screening — the compound serves as a defined test substance whose phenolic structure allows researchers to relate observed biological activity directly to the 2,5-dihydroxy substitution pattern.
- Antioxidant and radical-scavenging studies — the reductive character typical of dihydroxy phenolic compounds makes this molecule a suitable subject for assays examining radical capture behaviour in solution.
- Comparison standard in instrumental analysis — the material can be prepared as a reference solution for chromatographic or spectroscopic work where a known phenolic ester is required for peak comparison.
- Organic synthesis intermediate — the hydrolysable ethyl ester and two reactive hydroxyl groups provide multiple handles for building more complex phenolic derivatives in stepwise preparative work.
- Structure–activity relationship studies — comparing this ester against the free gentisic acid lets researchers assess how increased lipophilicity changes behaviour in membrane-based or cell-based test systems.
Specifications
- Brand: TCI
- Product code: E1559
- CAS number: 3943-91-7
- Category: Life Science > Biochemicals and Reagents > Bioactive Compounds
- Pack sizes: supplied in standard TCI research-scale packaging; please confirm the available pack size when ordering
- Storage: store according to the conditions stated on the manufacturer's label and safety data sheet
Handling and Storage
Store the container tightly closed in a cool, dry place away from direct sunlight, heat sources, and strong oxidising agents, following the storage conditions printed on the manufacturer's label and safety data sheet. Phenolic compounds are generally sensitive to air and light, so keep the original container sealed and return it promptly after weighing; amber glass or the supplier's original packaging is appropriate. Handle the material in a fume hood or a well-ventilated area, wearing safety glasses, gloves, and a laboratory coat. Avoid contact with skin and eyes and avoid inhaling any dust generated during weighing. Label all prepared solutions clearly with compound name, concentration, solvent, and preparation date, and dispose of residues through the laboratory's chemical waste route.
