TCI
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Description
TCI D4215 N,N'-Diacetylchitobiose is a disaccharide built from two N-acetylglucosamine units joined through a beta-1,4 glycosidic linkage. The compound represents the core structural fragment of chitin and of the N-type glycan chains found on glycoproteins, which places it in an important position within the field of glycoscience. In the laboratory, it serves as a reference standard and a model substrate for studying chitin-degrading enzymes, sugar-binding lectins, and the biosynthesis and processing pathways of glycans in cells, without the need to isolate that fragment from complicated natural sources.
The properties that make this material valuable are its structural purity and the clarity of its chemical definition. Unlike extracted chitin or chitosan, which are polydisperse and difficult to control in terms of chain length, this disaccharide has a single, well-defined structure, so enzyme kinetics data, lectin binding constants, and spectra obtained from it can be interpreted without ambiguity. Its hydrophilic character and solubility in aqueous media also make it straightforward to use in enzymatic assays and in buffer-based binding studies where the sample must dissolve cleanly and behave reproducibly.
Because it is supplied in a small 20 mg pack, the compound suits work at analytical scale, where only limited quantities are consumed per experiment. In Indonesian laboratories, this format fits research groups in university chemistry and biochemistry departments, biotechnology institutes, and enzyme research units that carry out characterisation work on individual samples rather than production-scale preparations. The small pack allows a defined reference material to be kept on hand for method development and repeated assay runs without holding a large inventory of a specialised glycan fragment.
Laboratory Applications
- Chitinase and chitin-degrading enzyme studies: the compound serves as a chemically defined model substrate, so hydrolysis can be followed and kinetic parameters determined without the ambiguity introduced by polydisperse chitin preparations.
- Lectin binding assays: as a pure N-acetylglucosamine disaccharide it acts as a defined ligand for sugar-binding proteins, allowing binding constants to be measured and interpreted against a single known structure.
- N-glycan biosynthesis and processing research: it represents the core fragment of N-type glycan chains, making it a suitable model for investigating how glycans are assembled and processed within cells.
- Reference standard in analytical and spectroscopic work: its single defined structure gives unambiguous spectra, so it can be used to confirm identity, calibrate methods, and validate the assignment of related carbohydrate signals.
- Glycoscience method development: the compound provides a reliable, reproducible starting material for establishing assay conditions in carbohydrate chemistry work before those methods are applied to more complex or less defined biological samples.
Specifications
- Brand: TCI
- CAS number: 35061-50-8
- Chemical name: N,N'-Diacetylchitobiose
- Category: Chemistry > Chemical Biology > Glycoscience
- Pack size: 20 mg
- Storage: store according to the manufacturer's stated conditions on the product label
Handling and Storage
Store the material in its original tightly closed container, following the storage conditions stated by the manufacturer on the product label and in the accompanying documentation. As a hydrophilic carbohydrate, it should be protected from moisture and kept in a dry place; allow a chilled container to reach room temperature before opening so that condensation does not enter the vial. Handle in a clean, dry working area using clean spatulas and dry glassware to avoid contaminating the remaining contents, and reseal the container promptly after each use. Given the small pack size, weigh out only the quantity required for the experiment at hand. Wear a laboratory coat, safety glasses, and suitable gloves during handling, and consult the manufacturer's safety data sheet before use and before disposal.
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