Glycosynthase (Endo-M-N175Q) Recombinant TCI G0365 is a specialized enzyme used in chemical biology and glycoscience research. This enzyme, derived from *Mucor hiemalis* and expressed in *Escherichia coli*, plays a crucial role in glycosylation reactions. It is designed to catalyze the formation of glycan and glycoprotein structures, making it an essential tool for researchers studying carbohydrate-based biomolecules. Its controlled structure and activity ensure reliable performance in laboratory settings, supporting the synthesis of complex carbohydrates with high precision.
The enzyme’s unique properties, such as high endonuclease activity and substrate specificity, make it a preferred choice for biochemical studies. The N175Q variant enhances stability and efficiency under laboratory conditions, reducing the risk of degradation and improving experimental reproducibility. Its ability to efficiently cleave glycan chains ensures accurate results in glycosylation-related experiments. These features make it ideal for applications requiring precise enzymatic control and minimal side reactions.
In Indonesian laboratories, this enzyme is widely used in genetic disease research, pharmacology, and biotechnology. Its role in glycan synthesis supports studies on protein glycosylation, which is critical for understanding cellular processes and disease mechanisms. Researchers rely on its reliability and performance to advance their work in biochemical and molecular biology fields.
- Glycan Synthesis: This enzyme is ideal for synthesizing complex glycans, providing controlled and efficient enzymatic activity for structural studies.
- Glycoprotein Research: Its specificity enables accurate modification of glycoproteins, aiding in the study of protein-carbohydrate interactions.
- Genetic Disease Studies: The enzyme supports research into carbohydrate-related genetic disorders by facilitating precise glycan synthesis.
- Pharmacological Applications: It is used to investigate the role of glycosylation in drug development and therapeutic targeting.
- Biotransformation Processes: Its endonuclease activity aids in the breakdown and modification of glycan structures for biotechnological applications.
| Brand | TCI |
|---|---|
| CAS number | 37278-88-9 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Chemical Biology > Glycoscience |
| Pack sizes | 100m units/vial |
| Physical form | Recombinant enzyme in lyophilized powder form |
| Storage | Store at -20°C to maintain enzymatic activity and stability |
This enzyme should be stored at -20°C to ensure optimal stability and activity. It is recommended to keep it in airtight, moisture-proof containers to prevent degradation. Avoid exposure to light and high humidity, as these can affect its performance. When handling, use appropriate personal protective equipment to ensure safety. The enzyme is best stored in a freezer with a consistent temperature to maintain its integrity. Always ensure that the storage area is secure and free from contaminants to preserve its quality for laboratory use.
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