Hyaluronidase from Bovine Testes TCI H0164

Description

Hyaluronidase from Bovine Testes is an enzyme isolated from bovine testicular tissue that catalyses the breakdown of hyaluronic acid, the principal glycosaminoglycan forming the extracellular matrix of connective tissue. By cleaving the glycosidic bonds along this polysaccharide chain, the enzyme lowers the viscosity of the matrix and increases tissue permeability. Within the TCI catalogue the product carries the code H0164 and is grouped under the glycoscience product line. In the laboratory, hyaluronidase is used for dissociating tissue into single-cell suspensions, preparing histological samples, studying glycosaminoglycan structure, and supporting a wide range of cell biology and reproductive biology research.

The principal characteristic that makes this enzyme a preferred choice is its catalytic activity, which works rapidly under near-physiological conditions, allowing tissue to be broken down without the harsh chemical treatments that would damage cells. Bovine testes are recognised as a source yielding hyaluronidase of high activity and have long served as the classical reference material in extracellular matrix research. Supplied as a solid preparation, the enzyme can be dissolved in aqueous buffer as required, allowing researchers to set working concentrations precisely. As with any protein preparation, however, its activity depends on careful handling and appropriate storage conditions being maintained throughout use.

In Indonesian laboratories, this enzyme is typically encountered in university research groups, biomedical and veterinary institutes, and cell culture facilities where primary cells must be released from tissue before culture. It also appears in histology and pathology preparation workflows, and in reproductive biology work where matrix digestion is part of established protocol. Because it is offered as a catalogue chemical from TCI, it is generally ordered as a research-grade reagent for defined experimental protocols rather than for routine bulk consumption.

Laboratory Applications

• Tissue dissociation into single-cell suspensions, where the enzyme digests the hyaluronic acid holding cells together so primary cells can be released intact for downstream culture work.
• Histological sample preparation, in which controlled digestion of matrix glycosaminoglycans clarifies tissue architecture and improves the accessibility of structures being examined under the microscope.
• Glycosaminoglycan structural studies, where the enzyme's defined cleavage of glycosidic bonds within hyaluronic acid chains generates fragments useful for characterising polysaccharide organisation.
• Cell biology research involving the extracellular matrix, since removing hyaluronic acid allows investigators to examine how matrix composition influences cell adhesion, migration, and behaviour.
• Reproductive biology investigations, a field in which testicular hyaluronidase has a long-established role and where matrix digestion forms part of many standard laboratory protocols.

Specifications

• Brand: TCI (catalogue code H0164)
• CAS number: 37326-33-3
• Category: Chemistry > Chemical Biology > Glycoscience
• Pack sizes: available in the pack sizes listed for this catalogue item; please refer to the product listing
• Physical form: solid preparation, soluble in aqueous buffer for preparation of working solutions
• Storage: store according to the conditions stated by the manufacturer for this enzyme preparation

Handling and Storage

As an enzyme preparation, this product should be stored under the cold conditions specified by the manufacturer and kept tightly closed in its original container to limit exposure to moisture and air. Keep the solid dry, and prepare aqueous solutions in clean buffer only as needed, since enzyme activity in solution declines more rapidly than in the solid state. Repeated warming and cooling should be avoided, as should repeated freeze-thaw cycles of prepared solutions. Handle the material in a well-ventilated area or laboratory fume hood, wearing gloves, safety glasses, and a laboratory coat, and avoid generating or inhaling dust when weighing the solid. Protein preparations may cause sensitisation on repeated exposure, so minimise direct skin contact. Always consult the manufacturer's safety data sheet before first use, and dispose of residues in accordance with institutional chemical and biological waste procedures.