1,2-Phenylenediamine Dihydrochloride from TCI is the dihydrochloride salt of o-phenylenediamine. Both amino groups in this salt are protonated and paired with chloride ions. TCI classifies it as a non-heterocyclic building block, and it is a practical alternative to the free base form. In the laboratory, researchers use it as a starting material for benzimidazoles and quinoxalines, as a chromogenic substrate in peroxidase enzyme assays, and as a derivatization reagent for α-dicarbonyl compounds. The salt form is often preferred when the work is done in aqueous solution.
The main reason to choose the dihydrochloride is its good solubility in water. The salt is also generally more resistant to air oxidation than free o-phenylenediamine. Because the amino groups are protonated, the material keeps better in storage and is easier to weigh accurately. It dissolves readily in aqueous buffers, for example when preparing ELISA substrate solutions. When a synthesis needs the free amine, adding a base releases the amino groups easily. The characteristic ortho reactivity used to close heterocyclic rings therefore stays fully available.
In Indonesia, university chemistry departments, research institutes and analytical laboratories often need this material for organic synthesis and bioanalytical work. Synthetic chemists use it to build nitrogen-containing heterocycles for teaching, thesis research and medicinal chemistry projects. Biochemistry and immunology laboratories use it to prepare substrate solutions for peroxidase-based detection. Analytical laboratories use it to derivatize α-dicarbonyl compounds before chromatographic analysis. A single reagent that serves synthesis, enzyme assays and analytical derivatization is useful for laboratories that share chemical stock across several research groups.
- Benzimidazole synthesis: the two adjacent amino groups condense with carboxylic acids or aldehydes to close the benzimidazole ring once a base releases the free amine.
- Quinoxaline preparation: reaction with 1,2-dicarbonyl compounds forms the quinoxaline ring system, and the ortho arrangement of the amino groups makes this ring closure efficient.
- Chromogenic peroxidase assays: the salt dissolves readily in aqueous buffers, so it is a convenient substrate for horseradish peroxidase detection in ELISA and similar enzyme assays.
- Derivatization of α-dicarbonyl compounds: it reacts with α-dicarbonyl analytes to form stable quinoxaline derivatives, which are easier to detect and measure in chromatographic analysis.
- Aqueous-phase reagent preparation: because it dissolves well in water and resists air oxidation better than the free base, laboratories can prepare stock solutions more reliably and consistently.
| Brand | TCI (product code P0171) |
|---|---|
| CAS number | 615-28-1 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Building Blocks > Non-Heterocyclic Building Blocks |
| Pack sizes | available in the pack sizes TCI offers for this product |
| Physical form | solid dihydrochloride salt of o-phenylenediamine |
| Storage | keep tightly closed and protect from air, moisture and light |
Store 1,2-Phenylenediamine Dihydrochloride in its original tightly sealed container in a cool, dry, well-ventilated place, away from direct light and heat. Amber glass or other light-protective, chemically resistant containers are suitable if the material is repackaged. Keep it away from strong oxidizing agents and strong bases. Close containers promptly after use to reduce exposure to air and moisture. Aromatic diamines and their salts need careful handling: wear gloves, safety glasses and a lab coat, weigh the powder in a fume hood or ventilated enclosure, and avoid creating or inhaling dust. Follow the supplier's safety data sheet and local regulations for handling and disposal.
—
Produk Terkait
-
N,N,N',N'-Tetramethyl-1,4-phenylenediamineDihydrochloride TCI T0151
Regular price From Rp 584.850,00Regular price -
1,2-PhenylenediamineDihydrochloride[forBiochemicalResearch] TCI P1144
Regular price Rp 610.050,00Regular price -
1,3-PhenylenediamineDihydrochloride TCI P0172
Regular price From Rp 689.850,00Regular price -
1,4-PhenylenediamineDihydrochloride TCI P0152
Regular price From Rp 848.400,00Regular price -
N-Methyl-1,2-phenylenediamineDihydrochloride TCI M1754
Regular price Rp 2.359.350,00Regular price -
N,N-Dimethyl-1,4-phenylenediamineDihydrochloride TCI D0780
Regular price Rp 2.889.600,00Regular price -
N,N-Diethyl-1,4-phenylenediamineDihydrochloride TCI D0519
Regular price From Rp 1.353.450,00Regular price -
N,N,N',N'-Tetrakis[4-(diisobutylamino)phenyl]-1,4-phenylenediamine TCI T3770
Regular price From Rp 2.094.750,00Regular price
![615-28-1 1,2-Phenylenediamine Dihydrochloride [for Biochemical Research] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510P1144.jpg?v=1767134361&width=533)
![485831-34-3 N,N,N',N'-Tetrakis[4-(diisobutylamino)phenyl]-1,4-phenylenediamine - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510T3770.jpg?v=1767144326&width=533)