Isomalto-oligosaccharides as High-Purity Standards for HPLC and MS Analysis and Enzyme Substrates

Isomalto-oligosaccharides as High-Purity Standards for HPLC and MS Analysis and Enzyme Substrates

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Isomalto-oligosaccharides (IMO) are linear oligosaccharides composed of glucose units linked by alpha-(1->6) glycosidic bonds. Isomaltose (1), isomaltotriose (2), isomaltotetraose (3), and isomaltopentaose (4) are high-purity isomalto-oligosaccharides obtained by hydrolysis of dextran, then separated and purified according to their chain length. Owing to their well-defined chemical structures and high purity, they are suitable as standard materials for HPLC and MS analyses, enzyme substrates, and various functional evaluations. Recent research interest includes prebiotic studies on selective utilization by bifidobacteria and lactic acid bacteria, evaluation of interactions with metal ions based on alpha-1,6-linked low-molecular-weight structures, and pharmaceutical research focusing on iron transport functions. Consequently, IMO are used across research areas, from basic to applied studies.

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