The Iwaki PA NESSLER50S Nessler colour comparison tube with marking is a cylindrical glass tube with a flat bottom, made for visual colorimetric testing. Analysts use it to compare the colour intensity of a sample solution against a standard solution, usually by looking down through the liquid column from above against a white background. The name "Nessler" comes from its classic use in determining ammonia with Nessler's reagent. Today it is used much more widely, including in pharmacopoeial limit tests and water quality checks. The marking on the tube makes it easy to fill every comparison tube to the same volume.
Laboratories choose this tube because its dimensions are uniform. The diameter and glass wall thickness are consistent from tube to tube, so each tube gives the same light path length, and colour comparisons stay fair and repeatable. The flat bottom and clear glass help the observer judge very small differences in colour. The marking comes in two colours, amber and white, so users can pick the line that is easiest to read for their solution colour and lighting. The 50 mL and 100 mL sizes let laboratories match the tube to the procedure they follow.
In Indonesian laboratories, Nessler tubes are commonly used in pharmaceutical quality control, environmental testing, water treatment, and teaching laboratories. Many of these labs run routine limit tests and simple colour comparisons without a spectrophotometer, or use visual comparison as a quick check alongside instrumental methods. A matched set of uniform tubes gives analysts consistent results across shifts and operators. It also supports documented procedures based on pharmacopoeial and water quality methods.
- Ammonia determination with Nessler's reagent: this is the tube's classic use, and the uniform light path lets the colour developed in the sample be compared directly against prepared standards.
- Pharmacopoeial limit tests: analysts compare sample and reference solutions side by side, and the consistent tube dimensions keep the visual comparison fair and repeatable.
- Water quality checks: the clear glass and flat bottom help analysts see small colour differences in treated or natural water samples viewed from above against a white background.
- Visual colorimetric comparison against standard series: the marking lets every tube in a series be filled to the same volume, so differences in liquid height do not bias the colour reading.
- Teaching and training in analytical chemistry: students can learn the principles of visual colorimetry and the importance of equal path length using a simple, robust, and easily understood tool.
| Brand | Iwaki |
|---|---|
| CAS number | — |
| Molecular formula | — |
| Purity | — |
| Category | Nessler Tube |
| Pack sizes | — |
| Physical form | — |
| Storage | — |
- Product code: PA NESSLER50S
- Form: cylindrical clear glass tube with a flat bottom and a fill-volume marking
- Marking colour options: amber or white
- Sizes: 50 mL and 100 mL
Store Nessler tubes clean and dry in a rack or padded box, kept upright and separate so the glass does not knock together. Keep them away from dust and strong chemical vapours, which could leave residues or affect the marking. Clean tubes promptly after use with a suitable laboratory detergent, rinse thoroughly with purified water, and let them dry before storage, because any residue can affect later colour comparisons. Do not use abrasive brushes or scouring agents, as scratches on the glass can scatter light. Handle the tubes carefully as glassware, check for chips or cracks before each use, and replace damaged tubes so the comparison set stays matched. Wear appropriate personal protective equipment when handling reagents such as Nessler's reagent.
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