5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research] TCI B1239

Chemical Identity

CAS No.: 6578-06-9
PubChem: CID 81059·SID 87564175
Formula: C15H15BrClN2O4P
Molecular weight: 433.62 g/mol
Purity: >98.0%(T)
Reference databases: ChemSpider·ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline
SMILES: CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br
InChIKey: QEIFSLUFHRCVQL-UHFFFAOYSA-N

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5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt is a chemical compound widely used in biochemical research for its role in enzyme-based detection systems. This compound is specifically designed for use as a substrate in reactions involving alkaline phosphatase, a key enzyme in various analytical techniques. Its function is to provide a visible or measurable response when enzymatic activity occurs, making it essential for detecting proteins and enzymes in biological samples. This material is commonly employed in laboratory settings where precise and reliable results are required for scientific analysis.

The compound is valued for its chemical stability and high reactivity, which ensure consistent performance across different experimental conditions. Its specificity towards alkaline phosphatase allows for accurate and reproducible results, making it a preferred choice for researchers. The ability to dissolve easily in common solvents further enhances its usability, as it simplifies preparation and application in various laboratory protocols. These properties contribute to its reliability and effectiveness in biochemical assays.

In Indonesian laboratories, this compound is frequently used in biomedical, pharmacological, and biotechnological research. Local researchers rely on it for developing detection methods that require high sensitivity and specificity. Its availability in solid form and ease of handling make it a practical choice for a wide range of experimental applications. The compound’s versatility and performance in diverse conditions support its widespread use in both academic and industrial research settings.

TCI brochures (PDF)

Substrates for Biochemical Assays 2025-04-25 · p. 1
Antibodies and Related Reagents 2026-07-29 · p. 24
Reagents for Biological Research 2025-03-11 · p. 17

Related TCI products

Biochemical Detection: This compound is ideal for biochemical detection due to its reactivity with alkaline phosphatase, enabling precise identification of enzymatic activity in complex biological samples.
ELISA Assays: It is commonly used in ELISA techniques to detect specific proteins, offering a reliable and sensitive method for immunoassays.
Western Blot Analysis: The compound supports Western blot procedures by providing a clear signal when enzymes interact with target proteins, enhancing the accuracy of protein detection.
Molecular Biology Research: Its role in enzyme-based reactions makes it a valuable tool in molecular biology for studying enzyme function and protein interactions.
Biomedical Diagnostics: It is applied in diagnostic research to develop and validate methods for detecting biomarkers in clinical and research settings.

Brand	TCI
CAS number	6578-06-9
Molecular formula	—
Purity	—
Category	Life Science > Antibodies > Antibody Supplementary Products
Pack sizes	Available in standard laboratory quantities
Physical form	Solid
Storage	Store in a cool, dry place away from light

This compound should be stored in a cool, dry environment, away from direct sunlight to maintain its chemical stability. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. It is important to ensure that the compound is stored in a secure location, away from incompatible substances. Proper labeling of storage containers is essential for safe handling and to prevent accidental exposure. Maintaining proper storage conditions ensures the compound remains effective for its intended applications in laboratory research.

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