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Description
TCI C2244 Cloxacillin Sodium Salt Monohydrate is the sodium salt monohydrate form of cloxacillin, a semisynthetic penicillin antibiotic supplied as a biochemical reagent for laboratory research. Cloxacillin belongs to the isoxazolyl penicillin group, a class engineered to resist penicillinase enzymes, so the compound remains active against bacteria that produce them. In microbiology laboratories, this distinctive property is what makes it a valuable test substance, whether as a member of a susceptibility testing panel or as a tool for differentiating bacterial strains according to their beta-lactam resistance patterns.
The properties that lead researchers to select this material begin with its resistance to hydrolysis by penicillinase, which arises from the bulky isoxazolyl side chain structure. Added to this is good solubility in water, a benefit of the sodium salt form. The monohydrate form presents a crystalline powder that is easier to handle and to weigh consistently. This combination simplifies the preparation of sterile stock solutions by membrane filtration as well as the preparation of dilution series for microplate assays. As a reagent-grade material, its consistency supports reproducibility of results across serial testing.
In Indonesian laboratories, the material fits naturally into routine microbiological workflows where beta-lactam behaviour needs to be characterised. Research groups, teaching laboratories, and analytical facilities use it where a penicillinase-stable penicillin is required alongside other antibiotics in a comparative panel. The 1 g and 5 g pack sizes accommodate both smaller method development work and laboratories running repeated assay series, allowing the reagent to be matched to the scale of the work at hand.
Laboratory Applications
Antimicrobial susceptibility testing panels: its penicillinase-stable structure provides a reference point distinct from penicillinase-labile penicillins, allowing clear interpretation of resistance profiles across tested isolates.
Bacterial strain differentiation studies: the compound helps distinguish isolates by their beta-lactam resistance patterns, since penicillinase-producing organisms respond differently to cloxacillin than to susceptible penicillins.
Microplate dilution assays: good water solubility from the sodium salt form allows accurate serial dilution series to be prepared, supporting quantitative comparison of antibacterial response across concentration ranges.
Sterile stock solution preparation: the readily soluble crystalline powder dissolves cleanly for membrane filtration sterilisation, giving reproducible working stocks for downstream microbiological experiments and repeated assay runs.
Beta-lactam resistance mechanism research: as a reagent-grade isoxazolyl penicillin, it serves as a defined tool substance for studying penicillinase activity and enzyme-mediated resistance behaviour in bacterial cultures.
Specifications
Brand: TCI
Product code: C2244
CAS number: 7081-44-9
Category: Life Science > Biochemicals and Reagents > Antibiotics
Store the material according to the manufacturer's stated conditions on the product label, keeping the container tightly closed and protected from moisture, since the sodium salt form is readily soluble and can take up water from humid air. Use clean, dry spatulas and weighing vessels, and return the container to its designated storage location promptly after weighing. Prepared stock solutions should be kept in clearly labelled, sealed containers appropriate for aqueous antibiotic solutions. As with all antibiotic reagents, handle the powder with gloves and eye protection, avoid generating dust, and work in a well-ventilated area or fume hood to prevent inhalation or skin sensitisation. Dispose of residues and contaminated consumables in accordance with local laboratory waste procedures.
