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Description
TCI C2248 Cephalexin Monohydrate is a first-generation cephalosporin antibiotic supplied in monohydrate form for laboratory use. The compound acts by inhibiting bacterial cell wall synthesis, specifically the peptidoglycan cross-linking step carried out by penicillin-binding proteins. In microbiology and biotechnology laboratories, cephalexin is used as a standard reagent for antibiotic susceptibility testing, as a selective component in growth media, and as a comparison substance in pharmaceutical analysis. Its availability matters for laboratories running routine testing as well as antimicrobial resistance research.
The characteristics that make it a widely chosen material are its notable spectrum of activity against Gram-positive bacteria and a portion of Gram-negative bacteria, combined with good stability as a monohydrate solid. The monohydrate form provides a fixed water-of-crystallization content, so weighed masses are more consistent than with forms that readily absorb ambient moisture. Its beta-lactam ring is both the centre of activity and the part most sensitive to heat, moisture, and extreme pH, which makes careful handling an important part of working with this compound.
In Indonesian laboratories, this material typically supports routine microbiological workflows in hospital, academic, and industrial settings: susceptibility panels, selective media preparation, and reference comparison in pharmaceutical quality work. Because ambient conditions in Indonesia are warm and humid for most of the year, the moisture sensitivity of the beta-lactam ring makes controlled storage and disciplined weighing practice particularly relevant for laboratories handling this reagent over an extended working period.
Laboratory Applications
- Antibiotic susceptibility testing: serves as a standard cephalosporin reference reagent, allowing laboratories to assess bacterial response to a first-generation cephalosporin under controlled and repeatable testing conditions.
- Selective growth media preparation: incorporated as a selective component in culture media, suppressing susceptible bacteria so that target organisms can be isolated and cultivated more reliably.
- Antimicrobial resistance research: supports studies on bacterial resistance mechanisms, since its action on penicillin-binding proteins provides a defined target for investigating cell wall synthesis inhibition.
- Pharmaceutical comparative analysis: used as a comparison substance in analytical work, giving laboratories a consistent cephalexin material against which sample results can be evaluated.
- Routine microbiological quality testing: suitable for repeated routine assays because the monohydrate solid offers stable, consistent weighing behaviour across multiple testing sessions over time.
Specifications
- Brand: TCI
- CAS number: 23325-78-2
- Category: Life Science > Biochemicals and Reagents > Antibiotics
- Pack sizes: available in standard TCI catalogue pack sizes; please confirm the currently offered packaging option when ordering
- Physical form: monohydrate solid with fixed water-of-crystallization content
- Storage: keep in a cool, dry place protected from heat and moisture
Handling and Storage
Store the material in a cool, dry location, away from direct sunlight, heat sources, and high humidity, since the beta-lactam ring is sensitive to heat, moisture, and extreme pH. Keep the compound in its original tightly closed container, or in a clean, well-sealed glass or chemically compatible vessel, and close the container promptly after each use to limit moisture uptake. Weigh the solid quickly and return it to storage without delay. Standard laboratory precautions apply: wear gloves, a lab coat, and eye protection, avoid the generation and inhalation of dust, handle the material in a well-ventilated area or fume hood, and wash hands thoroughly after handling. Label containers clearly and follow institutional procedures for disposal of antibiotic-containing waste.
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