2,3-Diaminonaphthalene [for Biochemical Research] TCI D4101

Name: TCI D4101 771-97-1 2,3-Diaminonaphthalene [for Biochemical Research]
Brand: TCI
SKU: TCI2510D410124587
Price: 1210650.0 IDR
Availability: InStock

Rp 1.210.650,00

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Berat 100mg

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Description

TCI D4101 2,3-Diaminonaphthalene [for Biochemical Research], CAS number 771-97-1, is a bicyclic aromatic compound carrying two neighbouring amino groups on the naphthalene skeleton. In the laboratory it is widely known as a fluorescence-based derivatization reagent: the ortho-diamine group reacts specifically with certain analytes to form a cyclic product that fluoresces strongly. For this reason, the compound has become an important tool in trace analysis, particularly for the determination of nitrite, nitric oxide, and selenium in biological and environmental samples whose concentrations are very low and difficult to detect by ordinary colorimetric methods.

The property that makes this reagent the preferred choice is the formation of derivative compounds that fluoresce with high intensity, so that the detection limit of a method can be pushed far below what conventional absorbance measurement can achieve. The selectivity of the ortho-diamine reaction toward the target analyte also reduces the matrix interference commonly encountered in biological samples. The compound is a solid that can be dissolved in organic solvents or dilute acid solution to prepare working solutions, which makes it straightforward to integrate into established fluorimetric and chromatographic procedures.

In Indonesian laboratories, this reagent is typically used where trace-level analytes must be quantified reliably: university and research-institute biochemistry laboratories studying nitric oxide metabolism, environmental laboratories screening water samples for nitrite, and analytical service laboratories determining selenium at low concentrations. It is generally handled as part of a fluorimetric workflow, where a small quantity of reagent is weighed out, dissolved fresh, and reacted with sample aliquots before measurement, so that a single package supports a long sequence of routine runs.

Laboratory Applications

Nitrite determination in water and biological samples: the ortho-diamine group reacts selectively with nitrite to give a strongly fluorescent product, allowing quantification at concentrations too low for routine colorimetric assays.
Nitric oxide research in biochemistry: because nitric oxide is measured indirectly through its reaction chemistry, this reagent provides the high-intensity fluorescent derivative needed to follow very small amounts in complex biological matrices.
Selenium trace analysis: the compound forms a fluorescent cyclic derivative with selenium species, making it suitable for environmental and biological samples where selenium is present only at trace concentrations.
Fluorimetric method development and validation: the strong fluorescence response and low detection limits make this reagent useful when a laboratory needs to establish or verify a sensitive analytical procedure for trace analytes.
Environmental sample screening: the selectivity of the ortho-diamine reaction reduces matrix interference, so laboratories can analyse difficult environmental and biological sample types with less sample clean-up before measurement.

Specifications

Brand: TCI (Tokyo Chemical Industry)
CAS number: 771-97-1
Product code: D4101
Category: Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Physical form: solid, soluble in organic solvents or dilute acid solution for the preparation of working solutions
Storage note: keep protected from light and air; grade is designated for biochemical research use

Handling and Storage

The main weakness of this compound is its sensitivity to light and to oxidation by air, which can cause changes in the material over time. Store the container tightly closed in a dark, cool place, and keep it in amber glass or another light-protecting container rather than in clear vessels. Working solutions should be prepared fresh and shielded from light until measurement. Handle the solid in a fume hood using gloves, safety glasses, and a laboratory coat, minimise exposure of the open container to air, and return it promptly to storage after weighing. Consult the manufacturer's safety data sheet before first use and follow your laboratory's chemical waste procedures for disposal.