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Description
TCI D4352 Aminopyrine, also known as aminophenazone or a substituted aminoantipyrine, is a pyrazolone compound that has long been recognised in pharmacology and is today widely used as a research reagent. In the laboratory setting, this compound serves primarily as a model substrate in drug metabolism studies, particularly the N-demethylation reaction catalysed by hepatic microsomal enzymes. Its role as a probe substrate makes it an essential tool for researchers who need to measure the activity of biotransformation enzyme systems, evaluate the effects of enzyme inducers and inhibitors, and understand the metabolic pathways of xenobiotic compounds in quantitative terms.
The property that makes aminopyrine a preferred choice is its pyrazolone structure bearing a dimethylamino group, a moiety that readily undergoes oxidative demethylation to release formaldehyde, which can be measured by simple and well-established colorimetric methods. This reaction produces a clear analytical signal, making the compound well suited to routine enzyme activity assays where reproducibility matters more than instrumental complexity. In addition, the compound dissolves well in aqueous media under common biochemical assay conditions, which simplifies the preparation of stock solutions and the dilution series required for kinetic work. TCI supplies this material with quality documentation appropriate for research use.
In Indonesian laboratories, this reagent is typically found in university pharmacology and pharmacy departments, biochemistry teaching and research laboratories, and institutional research centres working on drug metabolism and toxicology. It is commonly used in liver microsome preparations, in comparative studies of enzyme induction and inhibition, and in practical coursework where students learn to quantify biotransformation activity. Its use is intended strictly for experimental and research purposes, not for clinical or therapeutic application.
Laboratory Applications
- Hepatic microsomal N-demethylation assays — the dimethylamino group is cleanly demethylated by microsomal enzymes, giving a direct and reproducible readout of biotransformation activity in prepared liver fractions.
- Enzyme induction studies — researchers use it as a probe substrate to compare metabolic rates before and after treatment with inducing agents, quantifying how strongly the enzyme system responds.
- Enzyme inhibition screening — because the reaction rate is easily measured, the compound allows inhibitors to be ranked by the degree to which they suppress demethylation activity under controlled conditions.
- Colorimetric formaldehyde-based enzyme activity determination — the formaldehyde released during demethylation is quantified by established colour reactions, so no specialised instrumentation is required for routine measurement.
- Xenobiotic metabolism teaching and method development — its predictable behaviour and simple detection chemistry make it a dependable model substrate for training students and validating newly established assay protocols.
Specifications
- Brand: TCI (Tokyo Chemical Industry)
- CAS Number: 58-15-1
- Catalogue Number: D4352
- Category: Life Science > Biochemicals and Reagents > Pharmaceutical Research Reagents (for Experimental Use)
- Pack Sizes: available in standard TCI research pack sizes; please confirm the currently offered options when ordering
- Storage: store according to the conditions stated on the product label and Safety Data Sheet
Handling and Storage
Store the container tightly closed in a cool, dry, well-ventilated place, away from direct sunlight, moisture, and incompatible materials, following the storage conditions stated on the product label and Safety Data Sheet. Keep the material in its original supplier packaging or in a clean, chemically resistant and clearly labelled container. Handle the compound in a fume hood or a well-ventilated work area, and wear appropriate personal protective equipment including a laboratory coat, gloves, and safety glasses. Avoid inhalation of dust and direct contact with skin and eyes. Prepare stock solutions only in the quantities needed, record the preparation date, and dispose of residues and contaminated materials in accordance with applicable laboratory chemical waste procedures. This product is intended for experimental and research use only.
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