• 57-09-0 Hexadecyltrimethylammonium Bromide [for Biochemical Research] - AMI Scientific
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TCI

CAS 57-09-0

Hexadecyltrimethylammonium Bromide [for Biochemical Research] TCI H1251

Hexadecyltrimethylammonium Bromide [for Biochemical Research] TCI H1251
Rp 502.950,00
Rp 502.950,00
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TCI H1251 with CAS number 57-09-0 is Hexadecyltrimethylammonium Bromide, widely known by the abbreviation CTAB or as cetrimonium bromide, supplied in a grade intended specifically for biochemical research. The compound is a cationic surfactant built from a hydrophobic sixteen-carbon alkyl chain paired with a positively charged, strongly hydrophilic quaternary ammonium head group. This amphiphilic architecture is what allows CTAB to solubilise cell membranes, form micelles above a certain concentration, and interact strongly with negatively charged molecules such as nucleic acids and with charged particle surfaces.

The property that makes CTAB a first-choice reagent is its ability to form complexes with DNA while simultaneously dissolving proteins, polysaccharides, and membrane components. In solutions of high ionic strength the CTAB–DNA complex remains soluble, whereas at low ionic strength that same complex precipitates out. This salt-dependent behaviour is the foundation of reliable DNA isolation methods for plant samples that are rich in polysaccharides and phenolic compounds, where conventional lysis buffers tend to carry contaminants through into the final extract. As a cationic surfactant, CTAB additionally serves as a shape-directing agent in nanoparticle synthesis and as a micelle-forming agent in chromatographic work.

In Indonesian laboratories this reagent is typically used in molecular biology and plant biotechnology settings, where CTAB-based extraction protocols are the routine approach for recovering genomic DNA from leaf, seed, and other plant tissues. It is equally at home in university research groups, agricultural and plantation research institutes, and materials chemistry laboratories preparing nanostructured materials, where a consistent biochemical-research grade of the surfactant supports reproducible results across repeated preparations.

  • Plant genomic DNA extraction — the salt-dependent solubility of the CTAB–DNA complex lets polysaccharides and phenolic compounds be washed away, giving cleaner nucleic acid from difficult plant tissues.
  • Cell membrane lysis and solubilisation — the sixteen-carbon hydrophobic tail inserts into lipid bilayers and disrupts them, releasing intracellular contents together with proteins and membrane components in a single step.
  • Nanoparticle synthesis as a shape-directing agent — the cationic head group adsorbs selectively onto growing particle surfaces, guiding anisotropic growth and controlling the final morphology of the product.
  • Micellar chromatography and separation work — above its critical micelle concentration the surfactant forms micelles that act as a pseudo-stationary or mobile phase for separating analytes.
  • Nucleic acid complexation and precipitation studies — the strong electrostatic interaction between the quaternary ammonium head and the negatively charged DNA backbone makes it useful for controlled precipitation experiments.

Brand TCI
CAS number 57-09-0
Molecular formula —
Purity —
Category TCI
Pack sizes —
Physical form —
Storage store in a tightly closed container in a cool, dry place
  • Product code: H1251
  • Chemical name: Hexadecyltrimethylammonium Bromide (CTAB, cetrimonium bromide)
  • Grade: for Biochemical Research; pack sizes available on request

Store the container tightly closed in a cool, dry, well-ventilated place away from moisture, since this surfactant is hygroscopic in character and absorbed water can affect weighing accuracy and solution behaviour. Keep the material in its original supplier packaging or in a clean, chemically resistant closed container, clearly labelled and separated from strong oxidising agents. Handle the solid in a fume hood or a well-ventilated area to avoid generating and inhaling dust, and wear gloves, safety glasses, and a laboratory coat throughout weighing and solution preparation. Avoid contact with skin and eyes, wash hands thoroughly after handling, and follow the supplier safety data sheet together with your institution's chemical waste procedures for disposal of residues and rinsings.

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