Tetrabutylammonium Hydroxide (10% in Water) [Reagent for Ion-Pair Chromatography] TCI I0364

Chemical Identity

CAS No.: 2052-49-5
PubChem: CID 2723671·SID 87571717
Formula: C16H37NO
Molecular weight: 259.47 g/mol
Reference databases: ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: tetrabutylazanium hydroxide
SMILES: CCCC[N+](CCCC)(CCCC)CCCC.[OH-]
InChIKey: VDZOOKBUILJEDG-UHFFFAOYSA-M

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Tetrabutylammonium Hydroxide (10% in Water) is a specialized reagent used in analytical chemistry, particularly in ion-pair chromatography. This compound plays a crucial role in the separation of ions within chromatographic columns, enabling precise analysis of complex mixtures. It is widely utilized in laboratories for its ability to form ion pairs with analytes, enhancing resolution and detection in chromatographic processes. Its stability and compatibility with various analytical systems make it an essential tool in modern chemical analysis.

The chemical properties of Tetrabutylammonium Hydroxide make it a preferred choice for chromatographic applications. It is chemically stable and does not readily react with other reagents under normal laboratory conditions. Its 10% aqueous concentration ensures ease of handling and storage, while its non-toxic nature minimizes environmental and health risks. These characteristics contribute to its reliability and safety in laboratory settings, making it a trusted reagent for both qualitative and quantitative analysis.

In Indonesian laboratories, Tetrabutylammonium Hydroxide is commonly used in research institutions, universities, and industrial laboratories. It supports a wide range of analytical tasks, including the determination of ion composition in samples. Its ability to accelerate analysis without affecting sample integrity makes it a valuable asset in the field of analytical chemistry. The compound is particularly favored for its consistent performance and ease of integration into standard analytical workflows.

TCI brochures (PDF)

Ion-Pair Reagents for HPLC 2025-12-09 · p. 2

Related TCI products

Ion-Pair Chromatography: This reagent is ideal for ion-pair chromatography as it forms stable ion pairs with analytes, improving resolution and detection in complex mixtures.
Qualitative Analysis: It enables the identification of ions in samples by facilitating their separation in chromatographic systems.
Quantitative Analysis: Its consistent behavior allows for accurate quantification of ions in solution, making it suitable for precise analytical work.
Environmental Testing: Used in environmental laboratories to analyze water and soil samples for ion content and contamination.
Pharmaceutical Research: It supports the analysis of pharmaceutical compounds by enhancing ion separation in chromatographic processes.

From the TCI brochure

Ion-pair reagent for acidic samples in HPLC

Brand	TCI
CAS number	2052-49-5
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Chromatography Reagents and Solvents
Pack sizes	Available in standard laboratory quantities as per supplier specifications
Physical form	Aqueous solution
Storage	Store in a cool, dry place away from direct sunlight

Data from the TCI brochure

Reagent volume	12.5 mL (diluted to 1 L with an aqueous solvent such as methanol - water)
pH	7.5 (adjusted by addition of aqueous phosphoric acid (10%))

Source: TCI brochure A1084 (2025-12-09)

Tetrabutylammonium Hydroxide should be stored in a cool, dry place, away from direct sunlight and sources of heat. It is recommended to keep the reagent in a tightly sealed container to prevent evaporation and contamination. The solution is non-toxic and does not pose significant health risks, but standard laboratory safety practices should still be followed. It is compatible with most common laboratory glassware and plastic containers, provided they are suitable for aqueous solutions. Regular inspection of storage conditions is advised to ensure the reagent remains stable and effective for analytical use.

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