Sulfanilamide TCI S0119

Chemical Identity

CAS No.: 63-74-1
PubChem: CID 5333·SID 87575729
Formula: C6H8N2O2S
Molecular weight: 172.21 g/mol
Purity: >99.0%(HPLC)(T)
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 4-aminobenzenesulfonamide
SMILES: C1=CC(=CC=C1N)S(=O)(=O)N
InChIKey: FDDDEECHVMSUSB-UHFFFAOYSA-N

Rp 557.550,00

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CoA (Certificate of Analysis)

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Sulfanilamide from TCI is an aromatic sulfonamide compound recognized as one of the first synthetic antibacterial agents in the history of pharmacology. In the TCI catalog, it is listed under antibiotics within the life science biochemicals and reagents range. In the laboratory, sulfanilamide has two main roles. It serves as a reference compound in antibacterial activity studies, and it is a key component of the Griess reagent, which is widely used to determine nitrite levels in water, food, and biological samples.

Researchers choose this material because its mechanism of action is well documented. Sulfanilamide inhibits the bacterial enzyme dihydropteroate synthase by competing with p-aminobenzoic acid, which blocks folate synthesis. This makes it a reliable control in microbiology and antibiotic resistance research. In analytical chemistry, its primary aromatic amine group is readily diazotized by nitrite. The resulting diazonium product is then coupled with reagents such as N-(1-naphthyl)ethylenediamine to form an azo dye, and the color intensity can be measured spectrophotometrically.

In Indonesia, sulfanilamide is especially relevant to water quality testing laboratories, food testing laboratories, and research and teaching laboratories that work with microbiology or analytical chemistry. Laboratories that monitor nitrite in drinking water, environmental water, and processed food products use it routinely as part of colorimetric methods. University and institutional laboratories also use it as a well-characterized model compound when teaching or studying antibacterial mechanisms and sulfonamide chemistry.

TCI brochures (PDF)

Antimicrobials and Antitumor Agents for Research and Experimental Use 2025-04-24 · p. 6
Pharmaceutical Ingredients for Research and Experimental Use 2026-03-09 · p. 6

Related TCI products

Nitrite determination with the Griess method: sulfanilamide's primary aromatic amine reacts with nitrite to form a diazonium salt, which is the first step in this widely used colorimetric test.
Water quality analysis: laboratories testing drinking water and environmental water samples use sulfanilamide-based Griess reagent to measure nitrite reliably with standard spectrophotometric equipment.
Food testing: nitrite content in food samples can be determined with sulfanilamide-based colorimetry, which helps food testing laboratories run routine checks with an established method.
Antibacterial activity studies: because sulfanilamide's inhibition of dihydropteroate synthase is well documented, it works as a dependable reference compound when comparing the activity of new antibacterial candidates.
Antibiotic resistance research: microbiology laboratories use sulfanilamide as a control to study how bacteria develop resistance to sulfonamides and to agents that disrupt folate synthesis.

Brand	TCI (product code S0119)
CAS number	63-74-1
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Antibiotics
Pack sizes	available in several pack sizes; please contact our sales team to confirm the options
Physical form	solid; refer to the TCI certificate of analysis for lot-specific details
Storage	keep in a tightly closed container in a cool, dry place away from light

Store sulfanilamide in its original, tightly closed container in a cool, dry, well-ventilated area, protected from direct light and moisture. Keep it away from strong oxidizing agents and incompatible chemicals. When preparing Griess reagent solutions, use clean glassware and label every container clearly with its contents and preparation date. Wear appropriate personal protective equipment, including gloves, safety glasses, and a lab coat, and avoid creating or inhaling dust. Weigh the material in a well-ventilated area or under a fume hood. Always read and follow the TCI Safety Data Sheet for specific hazard, first-aid, and disposal information.

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