Sulfur-Transfer Reagents Xanthane Hydride and DDTT for Oligonucleotide Phosphorothioate Synthesis
Xanthane Hydride (X0001) and DDTT (D5920) are sulfur-transfer reagents for the synthesis of oligonucleotide phosphorothioates via the phosphoramidite method. Xanthane Hydride is used as a sulfur-transfer reagent and as a solution for DNA/RNA synthesizers, at 0.02 M in acetonitrile/pyridine (9:1) or 0.20 M in pyridine.³) ⁵) Its key advantage is that it does not yield oxidizing substances, carbon disulfide, or cyanamide as additional reaction products, which can cause side reactions and generate toxic substances. DDTT shares this advantage, and additionally has a dmf-protected amino group compared with X0001. It is expected to be more stable than X0001 in solution.⁶) Beaucage reagent (B3125) and bis(phenylacetyl) disulfide (B3623) are also widely used for similar purposes.¹) ²)
Related products
- TCI X0001 6846-35-1 Xanthane Hydride
- TCI D5920 1192027-04-5 N,N-Dimethyl-N'-(3-thioxo-3H-1,2,4-dithiazol-5-yl)formimidamide
- TCI B3125 66304-01-6 3H-1,2-Benzodithiol-3-one 1,1-Dioxide
Source
- TCI Product Highlights: www.tcichemicals.com/ID/en/product/tci-topics/ProductHighlights_20180709
- TCI product page X0001: tcichemicals.com/ID/en/p/X0001
- TCI product page D5920: tcichemicals.com/ID/en/p/D5920
- TCI brochure PP016: Sulfur-Transfer Reagents for Synthesis of Oligonucleotide Phosphorothioates
For research and laboratory use only, not for human consumption.
