Luminol [Chemiluminescence Reagent] TCI A5301

Chemical Identity

CAS No.: 521-31-3
PubChem: CID 10638·SID 87563135
Formula: C8H7N3O2
Molecular weight: 177.16 g/mol
Purity: >97.0%(HPLC)
Reference databases: ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 5-amino-2,3-dihydrophthalazine-1,4-dione
SMILES: C1=CC2=C(C(=C1)N)C(=O)NNC2=O
InChIKey: HWYHZTIRURJOHG-UHFFFAOYSA-N

Rp 662.550,00

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Luminol (521-31-3) is a chemical compound widely used in chemiluminescence reactions to detect the presence of heavy metals or specific compounds in samples. This reagent plays a crucial role in various laboratory settings, particularly in analytical chemistry, biochemistry, and environmental science. Its ability to emit light during chemical reactions makes it an essential tool for qualitative and quantitative analysis. Luminol is commonly employed in detection methods that require visual or instrumental observation of luminescent signals, offering a reliable and sensitive approach to sample analysis.

One of the key properties that make luminol a preferred choice is its chemical stability in solid form, which ensures consistent performance during experiments. Additionally, luminol exhibits high sensitivity to oxidizing agents such as hydrogen peroxide, allowing for the detection of trace amounts of target substances. Its luminescent properties are also highly visible, making it suitable for both manual and automated detection systems. These characteristics contribute to its widespread use in scientific research and laboratory testing.

In Indonesian laboratories, luminol is extensively used in scientific research, education, and environmental testing. It is commonly found in chemical, biological, and environmental laboratories, where it supports the detection of heavy metals and the analysis of various samples. Its availability in multiple packaging options ensures ease of use across different experimental scales and applications.

Advantages (TCI brochure)

Reacts with hydrogen peroxide in presence of metals such as iron to emit strong blue light
Emission wavelength 460 nm

TCI brochures (PDF)

Chemiluminescence Reagents 2025-01-23 · p. 3
Reactive Oxygen Species (ROS) and Oxidative Stress Research Reagents 2026-04-23 · p. 1
Reagents for Biological Research 2025-03-11 · p. 8

Related TCI products

Chemiluminescence Detection: Luminol is ideal for detecting heavy metals in environmental and biological samples due to its sensitivity and luminescent response.
Environmental Analysis: It is used to analyze water and soil samples for trace metal contamination, offering a reliable method for environmental monitoring.
Biochemical Research: Luminol supports biochemical assays by enabling the detection of specific compounds through luminescent signals in controlled experiments.
Educational Laboratories: It is a valuable teaching tool for demonstrating chemiluminescent reactions and their applications in analytical chemistry.
Forensic Science: Luminol is used in forensic investigations to detect trace evidence, such as bloodstains, due to its high sensitivity and visual luminescence.

From the TCI brochure

Luminol test for identification of blood stains in forensic science
Trace detection of hydrogen peroxide and metals catalyzing the reaction

Brand	TCI
CAS number	521-31-3
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Luminescent Compounds/Detection
Pack sizes	Available in various sizes to suit different experimental needs
Physical form	Solid powder
Storage	Store in a cool, dry place away from light and moisture

Data from the TCI brochure

Emission wavelength	460 nm

Source: TCI brochure L3036 (2025-01-23)

Luminol should be stored in a cool, dry place, away from light and moisture to maintain its chemical stability. It is recommended to use airtight containers to prevent exposure to humidity and air, which can affect its performance. Due to its sensitivity, it should be handled with care to avoid contamination or degradation. In laboratory settings, it is important to follow standard safety protocols when working with chemical reagents. Proper labeling and storage conditions ensure the reagent remains effective for analytical purposes. Always ensure that storage areas are well-ventilated and free from incompatible substances to maintain a safe working environment.

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