Bis(2,4-dinitrophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds by HPLC and FIA] TCI A5303

Chemical Identity

Other names: DNPO
CAS No.: 16536-30-4
PubChem: CID 3080704·SID 87563137
Formula: C14H6N4O12
Molecular weight: 422.22 g/mol
Purity: >98.0%(T)
Reference databases: ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: bis(2,4-dinitrophenyl) oxalate
SMILES: C1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])OC(=O)C(=O)OC2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-]
InChIKey: CBZOGAWUNMFXFQ-UHFFFAOYSA-N

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Bis(2,4-dinitrophenyl) Oxalate is a chemiluminescent reagent widely used in the laboratory for the detection and quantification of fluorescent compounds. This compound plays a crucial role in analytical chemistry, particularly in methods such as High-Performance Liquid Chromatography (HPLC) and Flow Injection Analysis (FIA). Its unique ability to induce chemiluminescent reactions makes it an essential tool for researchers aiming to measure trace amounts of fluorescent substances with high sensitivity and accuracy. The reagent is commonly employed in biochemistry and molecular biology to support advanced analytical techniques that require precise and reliable results.

The chemical properties of Bis(2,4-dinitrophenyl) Oxalate make it a preferred choice for analytical applications. It exhibits stability under normal laboratory conditions and maintains high reactivity, ensuring consistent performance in various analytical setups. Its complex molecular structure allows for specific interactions with fluorescent compounds, enhancing the accuracy of detection. These characteristics contribute to its reliability in both routine and specialized laboratory environments, making it a trusted reagent for analytical chemists and biochemists.

In Indonesian laboratories, Bis(2,4-dinitrophenyl) Oxalate is frequently used in research related to chemistry, pharmacy, and molecular biology. It supports the development of efficient analytical methods, especially in studies involving fluorescent compounds. Its application is widespread in academic and industrial settings where high precision and sensitivity are required for reliable data collection and analysis.

TCI brochures (PDF)

Chemiluminescence Reagents 2025-01-23 · p. 3
Reagents for Biological Research 2025-03-11 · p. 8

Related TCI products

Used in fluorescent compound detection due to its chemiluminescent properties, enabling sensitive and accurate quantification in HPLC and FIA.
Applied in biochemistry research for analyzing fluorescent molecules, providing reliable data for molecular interactions and structural studies.
Employed in pharmaceutical analysis for identifying and quantifying fluorescent compounds in drug development and quality control.
Utilized in molecular biology for detecting fluorescent markers in nucleic acid and protein analysis, supporting genetic and biochemical research.
Integrated into environmental analysis to detect fluorescent pollutants, aiding in the assessment of water and soil contamination.

Brand	TCI
CAS number	16536-30-4
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Luminescent Compounds/Detection
Pack sizes	Available in standard laboratory quantities as per supplier specifications
Physical form	Solid, typically in powder or crystalline form
Storage	Store in a cool, dry place away from light and moisture

Bis(2,4-dinitrophenyl) Oxalate should be stored in a cool, dry environment to maintain its chemical stability and reactivity. It is recommended to keep the reagent in a tightly sealed container to prevent exposure to moisture and air, which may affect its performance. The substance should be stored away from direct sunlight and sources of heat to avoid degradation. In laboratory settings, it is important to handle the reagent with appropriate personal protective equipment, such as gloves and safety goggles, to ensure safe handling. Proper labeling of storage containers is essential to prevent accidental exposure and ensure safe access. Regular monitoring of storage conditions helps maintain the integrity and effectiveness of the reagent for analytical applications.

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