Trifluoroacetic Acid (TFA) TCI A5711 is a chemical reagent widely used in laboratory settings for quantitative and qualitative analysis. It is a strong acid and serves as an ion-pair reagent in liquid chromatography and mass spectrometry (LC-MS) applications. Its primary role is to enhance ionization and stability of ions during detection processes, making it essential for accurate and reliable analytical results. TFA is commonly employed in analytical chemistry to improve the performance of chromatographic techniques and to facilitate the detection of complex compounds.
The properties of TFA that make it a preferred choice include its high acidity and chemical stability. These characteristics allow it to effectively ionize molecules that are difficult to ionize under normal conditions. Its stability under standard laboratory conditions ensures consistent performance and ease of handling. Additionally, TFA is compatible with various analytical instruments and methodologies, making it a versatile reagent for a wide range of applications. Its reliability and effectiveness contribute to its widespread use in both research and industrial laboratories.
In Indonesian laboratories, TFA is frequently used in analytical chemistry, pharmaceutical research, and environmental studies. It plays a crucial role in procedures that require liquid chromatography and ion detection techniques. The availability of TFA in the local market supports scientific research and testing by providing researchers with a reliable and essential reagent for their experiments.
Advantages (TCI brochure)
- Highly volatile ion-pair reagent allowing continuous LC-MS analysis
- Does not contaminate LC-MS interfaces
TCI brochures (PDF)
- Ion-Pair Reagents for HPLC 2025-12-09 · p. 4
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- Liquid Chromatography-Mass Spectrometry (LC-MS) for enhanced ionization and detection of complex compounds.
- Pharmaceutical Analysis to improve the ionization of analytes in drug development and quality control.
- Environmental Testing for the detection of trace organic pollutants in water and soil samples.
- Chemical Synthesis to act as a catalyst or reagent in organic reactions requiring acidic conditions.
- Analytical Chemistry for the preparation of ion-pair reagents in chromatographic separations.
From the TCI brochure
- Ion-pair reagent for LC-MS; separation of benzylamine and phenylalanine shown in brochure
| Brand | TCI |
|---|---|
| CAS number | 76-05-1 |
| Molecular formula | — |
| Purity | — |
| Category | Analytical Chemistry > Analytical Reagents > Chromatography Reagents and Solvents |
| Pack sizes | 500 mL, 1 L (standard sizes) |
| Physical form | Liquid |
| Storage | Store in a cool, dark place away from moisture and incompatible materials. |
Data from the TCI brochure
| Mobile phase | CH3OH / H2O = 40 / 60 containing 5mM IPC Reagent (Kaseisorb LC ODS 2000, 2.0 mm I.D. X 150 mm; flow 0.2 mL/min; 25°C; UV 254 nm; MS 1100 MSD (Agilent) ESI (Positive)) |
|---|
Source: TCI brochure A1084 (2025-12-09)
Trifluoroacetic Acid should be stored in a cool, dark location, away from moisture and incompatible substances. It is recommended to use sealed, corrosion-resistant containers such as glass or polyethylene to prevent degradation. Due to its strong acidic nature, it should be handled with appropriate personal protective equipment, including gloves, goggles, and a lab coat. In the laboratory, it should be kept in a well-ventilated area to minimize exposure. Access to this reagent should be restricted to trained personnel only to ensure safe handling and prevent accidental spills or contamination. Regular inspection of storage conditions is necessary to maintain its stability and effectiveness.
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![76-05-1 Trifluoroacetic Acid (ca. 0.5mol/L in Water) [Ion-Pair Reagent for LC-MS] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5711.jpg?v=1769141526&width=1445)