(+)-4-Cholesten-3-one TCI C1366

Chemical Identity

Other names: (+)-3-Oxo-4-cholestene
CAS No.: 601-57-0
PubChem: CID 91477·SID 87566378
Formula: C27H44O
Molecular weight: 384.65 g/mol
Purity: >95.0%(GC)
Reference databases: ChEBI·ChemSpider·ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one
SMILES: CC(C)CCCC(C)C1CCC2C1(CCC3C2CCC4=CC(=O)CCC34C)C
InChIKey: NYOXRYYXRWJDKP-GYKMGIIDSA-N
MDL: MDL00003663

Rp 3.207.750,00

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Dokumen Keamanan & Mutu

↓ Unduh SDS / MSDS (PDF resmi TCI)

CoA (Certificate of Analysis)

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(+)-4-Cholesten-3-one from TCI, product code C1366, is a steroid ketone closely related in structure to cholesterol. It is an oxidation product of cholesterol. One way it forms is through the enzyme cholesterol oxidase, which converts the hydroxyl group into a ketone and shifts the double bond to create a conjugated enone system. In life science and biochemistry laboratories, this compound is used as a standard, a substrate, or a test compound in sterol metabolism research. It is a useful reference material for studying how cholesterol is converted and processed.

Researchers choose this compound mainly because its conjugated unsaturated ketone system gives a distinctive ultraviolet absorption. This makes it easy to detect by UV spectrophotometry or by HPLC with a UV detector, which is much harder to do when the analyte is cholesterol itself. As a result, it is a practical reference for monitoring enzymatic reactions and steroid transformations, where clear and reliable detection matters. The compound has also been reported to show biological activity that is being studied in lipid metabolism research. Purity and optical rotation data are given in the TCI certificate of analysis supplied with each lot.

In Indonesia, (+)-4-Cholesten-3-one is relevant to biochemistry, pharmacy, microbiology, and nutrition laboratories that study cholesterol metabolism and enzyme characterisation. University research groups and institutional laboratories can use it as an analytical standard when developing UV or HPLC-UV methods, as a reference product in cholesterol oxidase assays, and as a test compound in studies of steroid transformation by microorganisms. Its well-defined identity under CAS number 601-57-0 helps keep research results consistent and traceable.

TCI brochures (PDF)

Regenerative Medicine Research Reagents 2016-03-18 · p. 1
Steroids 2026-07-28 · p. 7

Related TCI products

Cholesterol oxidase assays: the compound is the direct oxidation product of this enzyme, so it works well as a reference standard for confirming and measuring enzyme activity in kinetic studies.
HPLC-UV method development: its conjugated enone system absorbs UV light distinctively, which allows sensitive detection and clear peak identification in sterol analysis by chromatography.
UV spectrophotometric monitoring: researchers can follow the conversion of cholesterol in real time, because the product absorbs in the UV range where cholesterol is much harder to detect.
Sterol metabolism research: as a structurally close relative of cholesterol, it is used as a substrate or test compound for studying the pathways that transform sterols in biological systems.
Microbial steroid transformation studies: microbiology laboratories can use it as a reference compound to identify and measure products formed when microorganisms break down or modify cholesterol.

Brand	TCI (product code C1366)
CAS number	601-57-0
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Steroids
Pack sizes	as listed on this product page
Physical form	refer to the TCI product documentation
Storage	follow the storage conditions on the TCI label and certificate of analysis

Keep (+)-4-Cholesten-3-one in its original, tightly closed TCI container, in a cool, dry, well-ventilated place away from direct light and heat. Follow the storage temperature on the product label and the TCI certificate of analysis. When weighing or transferring the compound, use clean, dry glass or chemically compatible containers to avoid contamination. Handle it in line with good laboratory practice: wear gloves, safety glasses, and a lab coat, avoid creating dust, and do not inhale it or let it touch your skin or eyes. Read the Safety Data Sheet before use, and dispose of waste according to institutional and local regulations.

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