2,5-Dihydroxybenzoic Acid (DHB) is a chemical compound widely used as a matrix in MALDI-TOF Mass Spectrometry. It plays a crucial role in the ionization of organic molecules, particularly for analyzing compounds that cannot be ionized directly using other methods. In chemical and biochemical laboratories, DHB is essential for the rapid identification and characterization of complex molecules such as proteins, polypeptides, and lipids. Its ability to generate stable ions makes it a preferred choice in various analytical studies.
DHB is valued for its stable chemical and physical properties, as well as its excellent ionization capability. Its aromatic structure enables strong interaction with the laser in MALDI techniques, ensuring consistent and reliable results. The compound is also resistant to temperature and humidity, making it suitable for storage and use under various laboratory conditions. Its non-reactive nature ensures minimal degradation, preserving the accuracy of analytical outcomes.
In Indonesian laboratories, DHB is commonly used in biomedical, pharmacological, and analytical chemistry research. It is a key component in the study of biomolecules and is frequently employed in academic and industrial settings. Its reliability and effectiveness make it an essential tool for researchers working on complex molecular analysis.
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- MALDI-TOF Mass Spectrometry for the analysis of biomolecules due to its ability to generate stable ions and interact effectively with laser energy.
- Protein and polypeptide characterization in biochemical research because of its role in facilitating ionization of large organic molecules.
- Lipid analysis in analytical chemistry laboratories where accurate and reliable ionization is required for complex molecular structures.
- Biomedical research for the identification of biomarkers and molecular targets in health and disease studies.
- Pharmaceutical development for the analysis of drug compounds and their metabolites during formulation and quality control processes.
| Brand | TCI |
|---|---|
| CAS number | 490-79-9 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Building Blocks > Non-Heterocyclic Building Blocks |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid powder |
| Storage | Store in a cool, dry place away from direct sunlight |
DHB should be stored in a cool, dry place, away from direct sunlight and sources of heat. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its non-reactive nature, it does not require special handling beyond standard laboratory safety practices. However, it is advisable to work in a well-ventilated area and wear appropriate personal protective equipment. The compound is stable under normal laboratory conditions and does not degrade easily, ensuring consistent performance in analytical applications. Proper storage ensures the material remains effective for its intended use in mass spectrometry and other analytical techniques.
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