N,N-Dimethyl-1,4-phenylenediamine Dihydrochloride [for Biochemical Research] TCI D3931

Chemical Identity

Other names: DMPD·2HCl · 4-Dimethylaminoaniline Dihydrochloride · N,N-Dimethyl-p-phenylenediammonium Dichloride · DMPD.2HCl
CAS No.: 536-46-9
PubChem: CID 2724166·SID 87561606
Formula: C8H12N2.2HCl
Molecular weight: 209.11 g/mol
Purity: >99.0%(HPLC)(T)
Storage: 0-10°C
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 4-N,4-N-dimethylbenzene-1,4-diamine;dihydrochloride
SMILES: CN(C)C1=CC=C(C=C1)N.Cl.Cl
InChIKey: IAEDWDXMFDKWFU-UHFFFAOYSA-N
MDL: MDL00012991

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TCI D3931 N,N-Dimethyl-1,4-phenylenediamine Dihydrochloride [for Biochemical Research] is the dihydrochloride salt of a para-phenylenediamine derivative that carries two methyl groups on one of its nitrogen atoms. The compound is a classic chromogenic reagent, and its role in the laboratory is to develop colour when it undergoes oxidation or reacts with certain analytes, so that the presence of a substance that was originally colourless can be followed visually or measured quantitatively with a spectrophotometer. This product class is categorised as a supplementary material for biochemical research and antibody-based work.

The property that makes this reagent a preferred choice is the ease with which it is oxidised into a strongly coloured species, combined with its good solubility in water thanks to the dihydrochloride salt form. The salt form also makes the powder more stable to handle and weigh than the free base, which darkens in air far more readily. Because the response is sensitive and relatively rapid, the reagent is suited both to qualitative bench-top tests and to colorimetric methods that require absorbance readings. The electron-donating character of the dimethylamino group is what underlies this colour formation behaviour.

In Indonesian laboratories, this reagent fits routine analytical and biochemical research work where a colour-forming indicator is needed to confirm or measure a reaction. It is typically used in university research laboratories, biochemistry and life science units, and analytical laboratories that run colorimetric procedures. Because it serves as a supplementary product for antibody-related work, it is generally kept on hand as part of the reagent stock supporting detection and staining workflows rather than as a bulk consumable.

TCI brochures (PDF)

Substrates for Biochemical Assays 2025-04-25 · p. 5
Antibodies and Related Reagents 2026-07-29 · p. 23
Reagents for Biological Research 2025-03-11 · p. 19

Related TCI products

Colorimetric assay development: the reagent forms a strongly coloured oxidation product, allowing colourless analytes to be quantified by absorbance measurement on a standard laboratory spectrophotometer.
Qualitative bench-top screening tests: because the colour response is sensitive and relatively rapid, results can be judged visually without instrumentation during preliminary or routine confirmation work.
Antibody-based detection support: it is classified as an antibody supplementary product, so it serves as the colour-generating component in detection systems built around antibody reagents.
Oxidation-based indicator chemistry: the electron-donating dimethylamino group makes the molecule readily oxidised, so it acts as a reliable visual indicator of oxidising conditions in a reaction system.
General biochemical research reagent preparation: its good water solubility in the dihydrochloride form makes it convenient to dissolve into aqueous working solutions for biochemical experiments.

From the TCI brochure

Combined with N0864 for o-Dianisidine (footnote *2)

Brand	TCI
CAS number	536-46-9
Molecular formula	—
Purity	—
Category	Life Science > Antibodies > Antibody Supplementary Products
Pack sizes	available in standard TCI catalogue pack sizes; please confirm the required size when ordering
Physical form	solid powder (dihydrochloride salt)
Storage	keep in a tightly closed container, protected from air and light

Grade / designation: for Biochemical Research

Store the reagent in a tightly closed container, protected from air and light, and keep it in a cool, dry place away from oxidising agents. Amber glass bottles or the original supplier container are suitable, and the container should be reclosed promptly after each use because phenylenediamine derivatives darken on exposure to air. Weigh the powder in a well-ventilated area or fume hood, and prepare aqueous solutions fresh where the method allows. Wear gloves, safety glasses and a laboratory coat during handling, avoid contact with skin and eyes and avoid inhaling dust, and dispose of residues and contaminated materials according to the chemical waste procedures of your laboratory.

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