3,3'-Diaminobenzidine Tetrahydrochloride Hydrate [for Biochemical Research] TCI D3757

Name: TCI D3757 868272-85-9 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate [for Biochemical Research]
Brand: TCI
SKU: TCI2510D375724121
Price: 1417500.0 IDR
Availability: InStock

Rp 1.417.500,00

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Berat 1g 5g

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Description

TCI D3757 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate is the hydrated tetrahydrochloride salt form of DAB, prepared specifically for biochemical research and classified within antibody supplementary products. Like its free base counterpart, this compound functions as a chromogenic substrate for peroxidase enzymes, particularly horseradish peroxidase conjugated to secondary antibodies. When the enzyme acts in the presence of hydrogen peroxide, DAB is oxidised and precipitates as an insoluble brown reaction product, allowing the position of an antigen in tissue or a protein band on a membrane to be observed directly by eye or under an ordinary light microscope.

The principal advantage of the tetrahydrochloride salt form lies in its ease of handling. As a salt, this compound is considerably more soluble in water and aqueous buffers than the free base, so working solutions can be prepared quickly without the need for additional organic solvents. The salt form is also generally more stable against oxidation during storage, which reduces the risk of the material darkening before it is used. The properties of the final reaction product remain equally advantageous: the brown precipitate that forms adheres strongly and resists dehydration and subsequent processing steps.

In Indonesian laboratories, this product is typically used wherever peroxidase-based antibody detection is routine. It serves immunohistochemistry benches in hospital and university pathology units, research groups working on tissue localisation studies, and laboratories performing membrane-based protein detection. Because the salt dissolves readily in aqueous buffer, it suits facilities that prefer straightforward reagent preparation, and the stable brown product allows slides and membranes to be archived and re-examined long after the staining run is complete.

Laboratory Applications

Immunohistochemistry on tissue sections — the insoluble brown precipitate marks antigen position directly on the slide and remains visible under an ordinary light microscope without specialised optics.
Western blot and membrane-based protein detection — DAB oxidation produces a permanent brown band on the membrane, giving a visual record of the target protein that needs no imaging equipment.
Horseradish peroxidase conjugate detection systems — the compound acts as the chromogenic substrate for HRP linked to secondary antibodies, completing the enzyme-based signal generation step.
Routine diagnostic and teaching histology preparations — because the reaction product resists dehydration and further processing, stained slides can be mounted, archived and reviewed repeatedly.
General biochemical research on peroxidase activity — the salt dissolves quickly in aqueous buffer, so working solutions for enzyme-linked assays can be prepared without organic solvents.

Specifications

Brand: TCI
CAS number: 868272-85-9
Category: Life Science > Antibodies > Antibody Supplementary Products
Product code: D3757
Chemical form: tetrahydrochloride salt, hydrate; grade stated for biochemical research
Pack sizes: available in the pack sizes offered by the manufacturer for this catalogue item
Storage: store as directed for the product; keep protected from light and air to limit oxidation

Handling and Storage

Keep the container tightly closed and store it in a cool, dark place, since DAB salts darken on exposure to light and air. Original manufacturer packaging or amber glass with a well-sealed cap is suitable; transfer only the quantity needed for immediate use and reseal promptly. Prepare working solutions fresh in aqueous buffer and handle them in a fume hood where practicable. Wear gloves, a laboratory coat and eye protection, avoid skin contact and inhalation of the powder, and collect DAB-containing waste separately for disposal according to institutional chemical waste procedures.