DAPI 2HCl [for Biochemical Research] TCI A2412

Chemical Identity

Other names: DAPI 2HCl
CAS No.: 28718-90-3
PubChem: CID 160166·SID 135727109
Formula: C16H17Cl2N5
Molecular weight: 350.2 g/mol
Purity: >90.0%(HPLC)
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride
SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
InChIKey: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Rp 1.590.750,00

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DAPI 2HCl is a chemical compound widely used in biochemical research to enhance the visualization of cellular structures and nuclear components. It serves as a fluorescent dye that binds specifically to DNA, making it an essential tool in cell staining applications. In the laboratory, DAPI 2HCl is employed to stain cell nuclei, allowing researchers to observe changes in morphology and genetic activity under fluorescence microscopy. Its ability to provide high-resolution images makes it a valuable asset in various biological studies.

One of the key properties that make DAPI 2HCl a preferred choice is its strong fluorescence characteristics. This enables clear and detailed imaging of cellular nuclei, even at low concentrations. Additionally, DAPI 2HCl is stable under certain conditions and resists degradation, ensuring consistent results across experiments. Its non-toxic nature also allows for safe use in sample preparation without compromising cell integrity, making it suitable for a wide range of applications in life sciences.

In Indonesian laboratories, DAPI 2HCl is commonly used in biomedical research, pharmacology, and cell biology. It is particularly favored in academic institutions and research centers that require microscopic analysis of cellular structures. Its reliability and effectiveness have made it a standard component in many experimental protocols, supporting both basic and applied research in the life sciences.

Advantages (TCI brochure)

DAPI fluorescence is detected in central root cells after TOMEI-II treatment (Fig 3f).

TCI brochures (PDF)

Rapid Transparency Reagent for Plants TOMEI 2024-02-16 · p. 2
Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation iTOMEI 2023-06-06 · p. 2
Cell Biology Reagents 2025-07-23 · p. 2
Cell Imaging Reagents 2026-06-25 · p. 2
Organelle Research Reagents 2026-08-06 · p. 2
Streptavidins and Related Reagents 2025-02-28 · p. 1

Related TCI products

Fluorescent Cell Staining: DAPI 2HCl is ideal for staining cell nuclei, enabling clear visualization under fluorescence microscopy for detailed cellular analysis.
Nuclear Imaging Studies: Its strong fluorescence allows researchers to study nuclear morphology and DNA distribution in various cell types with high precision.
Genetic Activity Monitoring: DAPI 2HCl helps in observing changes in genetic activity by highlighting nuclear structures during cell division or stress responses.
Microscopy Research: It is widely used in fluorescence microscopy to enhance the contrast and clarity of cellular components, especially in biomedical and pharmacological studies.
Sample Preparation for Analysis: Its non-toxic properties make it suitable for preparing biological samples without disrupting cellular structures for further analysis.

From the TCI brochure

Nuclear staining in fluorescence observation of TOMEI-cleared samples

Brand	TCI
CAS number	28718-90-3
Molecular formula	—
Purity	—
Category	Life Science > Cell Culture > Cell Staining
Pack sizes	Available in various quantities as per standard laboratory supply
Physical form	Solid or powder, depending on formulation
Storage	Store in a cool, dark place away from moisture and light

Data from the TCI brochure

Konsentrasi pewarnaan	5 μg/mL, 30 menit (DAPI staining (footnote 1, Application 1 and 3, Application 2))

Source: TCI brochure LL055 (2024-02-16)

DAPI 2HCl should be stored in a cool, dark environment to maintain its stability and prevent degradation. It is recommended to keep the compound in a tightly sealed container to avoid exposure to moisture and light, which can affect its fluorescence properties. In a laboratory setting, it is important to handle DAPI 2HCl with care, using appropriate protective equipment such as gloves and safety goggles. While it is generally non-toxic, it is advisable to follow standard laboratory safety protocols to ensure safe handling and minimize any potential risks. Proper storage and handling will help preserve the integrity and effectiveness of the compound for long-term use in research applications.

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