Disodium 4-Nitrophenyl Phosphate Hexahydrate [for Biochemical Research] TCI D4005

Name: TCI D4005 333338-18-4 Disodium 4-Nitrophenyl Phosphate Hexahydrate [for Biochemical Research]
Brand: TCI
SKU: TCI2510D400524464
Price: 827400.0 IDR
Availability: InStock

Rp 827.400,00

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Berat 1g 5g

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Description

Disodium 4-Nitrophenyl Phosphate Hexahydrate, commonly abbreviated as pNPP, is a chromogenic substrate that forms the backbone of many phosphatase-based enzymatic assays in biochemical laboratories. The compound acts as a phosphate group donor that is hydrolysed by alkaline phosphatase and acid phosphatase alike, releasing 4-nitrophenol, which develops a bright yellow colour under alkaline conditions. That colour change is measured spectrophotometrically, allowing enzyme activity to be quantified in a way that is simple, rapid, and free of complicated instrumentation. In everyday practice, pNPP serves as the bridge between an enzyme reaction that cannot be seen and an optical signal that is easy to read.

The characteristics that make this material such a widely chosen reagent are its good solubility in water and aqueous buffers, its stability as a hydrated disodium salt, and its low background absorbance before the reaction proceeds, so that the signal-to-noise ratio is preserved. The hexahydrate form makes weighing and dissolution straightforward at small scale, while the hydrolysis product carries a strong absorption coefficient in the visible wavelength region. The grade offered here is intended for biochemical research, matching the demands of work where reaction responses must be consistent and reproducible from one run to the next.

In Indonesian laboratories, pNPP is routinely used across university biochemistry teaching and research labs, clinical and diagnostic development settings, and enzyme-focused work in food, agricultural, and environmental laboratories. It appears wherever phosphatase activity has to be measured with a plate reader or a conventional spectrophotometer, and it is equally common as the detection substrate in alkaline phosphatase-conjugated immunoassay workflows, where it supports antibody-based detection alongside other antibody supplementary products.

Laboratory Applications

Alkaline phosphatase activity assays — pNPP is hydrolysed efficiently by the enzyme and yields a bright yellow product under alkaline conditions, so activity can be quantified directly by absorbance.
Acid phosphatase activity assays — the same substrate serves acid phosphatase reactions, letting a single reagent cover both enzyme classes and simplifying reagent inventory in a working biochemistry laboratory.
ELISA and immunoassay detection — as a chromogenic substrate for alkaline phosphatase-conjugated antibodies, it converts specific binding events into a readable optical signal in microplate formats.
Enzyme kinetics and inhibitor screening — its low background absorbance and strong product absorption give a clean, linear optical readout suitable for rate measurements and inhibition studies.
Biochemistry teaching and method development — good aqueous solubility and easy small-scale weighing in the hexahydrate form make it practical for student practicals and for validating new assay protocols.

Specifications

Brand: TCI
Product code: D4005
CAS Number: 333338-18-4
Category: Life Science > Antibodies > Antibody Supplementary Products
Physical form: hydrated disodium salt (hexahydrate), water-soluble
Grade: for biochemical research
Pack sizes: available in standard TCI catalogue pack sizes — please confirm the size required when ordering
Storage: store as directed on the product label; keep containers tightly closed and protected from light and moisture

Handling and Storage

Store the material in its original tightly closed container in a cool, dry place, protected from light and moisture, since the hydrated salt is hygroscopic in character and its assay performance depends on an intact, low-background reagent. Amber glass or opaque containers are preferred for prepared stock solutions, and solutions should be made fresh in the appropriate buffer where assay sensitivity matters. Handle the powder in a well-ventilated area or fume hood, wearing gloves, safety glasses, and a laboratory coat, and avoid generating dust. Use clean, dry spatulas to prevent moisture ingress into the bulk container, label all prepared solutions clearly with the preparation date, and follow the manufacturer's safety data sheet together with your institutional waste-handling procedures for disposal.