TCI D5812 Dimethylenastron is a small-molecule inhibitor widely used as a research tool in cell biology and cancer research. The compound belongs to the tetrahydropyrimidinone class of derivatives developed from the monastrol scaffold, and it is known as an inhibitor of kinesin spindle protein (KSP/Eg5), the motor protein responsible for separating centrosomes and establishing the bipolar mitotic spindle. In the laboratory, its role is to serve as a pharmacological tool for disrupting cell division in a specific manner, allowing researchers to study the consequences of mitotic inhibition in living cells.
The characteristic that makes this compound a preferred choice is the selectivity of its mechanism. Unlike classical antimitotic agents that act directly on microtubules and therefore also affect non-dividing cells, Eg5 inhibitors target a motor protein that functions only during mitosis. The typical result is the formation of monoastral spindles and the arrest of cells in the mitotic phase, a phenotype that is easily recognised under the microscope and serves as a very clear indicator that the compound is working. As a cell-permeable small molecule, it acts on cultured cells without the need for special delivery systems.
In Indonesian laboratories, this compound is typically used in university research groups, biomedical institutes, and cancer research units that maintain cell culture facilities. It is generally applied to cultured cell systems as a mechanistic probe rather than as a routine reagent, supporting studies where the effects of mitotic arrest need to be observed directly. Because it is supplied by TCI as a catalogue research chemical, it is normally ordered in small quantities for defined experimental programmes and stored under the conditions specified by the manufacturer.
- Mitotic arrest studies — the compound blocks Eg5 motor activity, halting cells in mitosis so researchers can examine cell cycle checkpoints and the downstream consequences of prolonged mitotic delay in living cultures.
- Spindle assembly research — inhibition of centrosome separation produces characteristic monoastral spindles, giving cell biologists a reliable and reproducible model for studying how bipolar spindle architecture is normally established.
- Cancer research screening — because dividing tumour cells depend heavily on Eg5 activity, this inhibitor is used as a reference tool compound when evaluating antimitotic strategies and comparing candidate agents in cultured cancer cell lines.
- Mechanism-of-action comparison studies — its motor-protein-specific action allows direct comparison against microtubule-targeting agents, helping researchers distinguish spindle-specific effects from broader cytoskeletal disruption in non-dividing cells.
- Live-cell imaging experiments — the monoastral spindle phenotype is easily recognised under the microscope, making this compound well suited to time-lapse and fluorescence imaging work that requires a clear, visually verifiable readout.
| Brand | TCI (Tokyo Chemical Industry) |
|---|---|
| CAS number | 863774-58-7 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Cell Biology > Cancer Research |
| Pack sizes | supplied in TCI research-scale catalogue pack sizes; storage as specified on the manufacturer label and certificate of analysis |
| Physical form | — |
| Storage | — |
- Catalogue reference: D5812
- Product type: small-molecule KSP/Eg5 inhibitor, cell-permeable research reagent
Store the compound according to the temperature conditions stated on the manufacturer's label, keeping the container tightly closed and protected from moisture and light. Keep the material in its original supplier container or in a suitable tightly sealed vial, and allow it to reach room temperature before opening to reduce condensation. Prepare stock solutions in a clean laboratory environment and aliquot them to avoid repeated freeze–thaw cycles. Handle the compound as a biologically active research chemical: wear gloves, a laboratory coat, and eye protection, weigh and dispense in a fume hood or suitable enclosure, avoid inhalation of dust and contact with skin, and dispose of residues and contaminated consumables through the laboratory's chemical waste procedures. For research use only, not for diagnostic or therapeutic applications. Consult the safety data sheet before use.
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