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Description
Ethylene Glycol Bis(2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid, commonly known as EGTA, is a chemical compound widely used in biochemical and molecular biology research. It functions as a metal chelating agent, specifically designed to bind calcium ions with high affinity. EGTA is essential in laboratory settings where precise control of calcium ion concentration is required. Its ability to selectively sequester calcium makes it a critical reagent in experiments involving cellular signaling, enzyme activity, and physiological processes dependent on calcium regulation.
EGTA is favored for its chemical stability and specificity. Unlike other chelating agents, it does not bind to other metal ions such as magnesium or iron, ensuring that only calcium is targeted. This selectivity is crucial in maintaining the integrity of experimental conditions. Additionally, EGTA is highly soluble in water, making it easy to incorporate into various laboratory solutions. These properties make it an ideal choice for researchers aiming to achieve precise and controlled environments in their experiments.
In Indonesian laboratories, EGTA is extensively used in cell culture and biochemical research. It is commonly employed in studies that require the manipulation of intracellular calcium levels, such as in the investigation of cellular responses and signaling pathways. Its reliability and effectiveness have made it a preferred reagent for both academic and industrial research in the region.
Laboratory Applications
Cell Culture Experiments: EGTA is used to regulate calcium ion concentrations in cell culture media, enabling precise control over cellular signaling and enzyme activity.
Biochemical Research: It is essential in experiments where calcium ion binding is required to study enzyme kinetics and protein interactions.
Molecular Biology Studies: EGTA helps in creating controlled environments for studying calcium-dependent processes in molecular biology.
Enzyme Activity Assays: It is used to modulate calcium levels in assays to assess the effect of calcium on enzymatic reactions.
Signal Transduction Studies: EGTA is employed to investigate calcium-dependent signaling pathways in cellular responses.
Specifications
Brand: TCI
CAS Number: 67-42-5
Category: Life Science > Cell Culture > Reagents and Supplements
Pack Sizes: Available in various quantities as per supplier specifications
Physical Form: Solid powder
Storage Note: Store in a cool, dry place away from moisture and light
Handling and Storage
EGTA should be stored in a cool, dry place, away from moisture and direct light to maintain its chemical stability. It is recommended to use airtight containers to prevent exposure to humidity, which could affect its solubility and effectiveness. As a solid powder, it should be handled with care to avoid inhalation or skin contact. In laboratory settings, it is advisable to use gloves and a fume hood when preparing solutions to ensure safety. Proper labeling of containers is essential to prevent cross-contamination and ensure safe handling. Always follow standard laboratory safety protocols when working with chelating agents to maintain a safe and controlled environment.
![Ethylene Glycol Bis(2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid [for Biochemical Research] (CAS 67-42-5) - TCI E0805 - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510E0805.jpg?v=1768818736&width=1946)
![Ethylene Glycol Bis(2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid [for Biochemical Research] (CAS 67-42-5) - TCI E0805 - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510E0805.jpg?v=1768818736&width=1445)
