Ethyl Gallate is the ethyl ester of gallic acid, supplied specifically for the determination of total polyphenol content. As a phenolic compound bearing three hydroxyl groups on its aromatic ring, it serves as a reference material in colorimetric methods that measure the total phenolic content of food samples, beverages, plant extracts, and herbal products. The availability of a well-characterized analytical standard is essential so that measurement results can be fairly compared between laboratories and so that the calibration curves constructed genuinely represent the expected analytical response.
The property that makes this compound suitable as a standard is its better stability compared with several other polyphenols that degrade easily, together with its consistent response toward the Folin–Ciocalteu reagent and similar colorimetric methods. As an ester, the compound has adequate solubility in the water–ethanol or water–methanol mixtures commonly used to dissolve polyphenol standards. Its galloyl group also confers strong radical-scavenging activity, so the same compound is frequently used as a comparator in antioxidant activity testing.
In Indonesian laboratories, this standard is typically used where total polyphenol figures must be reported reliably: food and beverage quality control units, herbal and jamu product laboratories, agricultural and plantation commodity testing, and university research groups working on plant extracts. Because it is packaged as a defined analytical standard from TCI, it fits routine calibration work as well as method verification, where a stable and reproducible reference response is required across repeated analytical runs.
- Total polyphenol determination by the Folin–Ciocalteu method, where its consistent colorimetric response allows construction of calibration curves that accurately represent the phenolic content of the sample.
- Quality control of foods and beverages, since reported total polyphenol figures require a well-characterized standard so that results remain comparable between production batches and between testing laboratories.
- Analysis of plant extracts and herbal products, where the compound's stability compared with more easily degraded polyphenols makes it a more dependable day-to-day reference material for phenolic assays.
- Antioxidant activity testing, because the galloyl group present in the molecule provides strong radical-scavenging activity, making the compound a useful comparator alongside total phenolic measurements.
- Analytical method verification and inter-laboratory comparison, where a reproducible reference response is needed to confirm that the colorimetric procedure is performing as expected before routine samples are run.
| Brand | TCI (Tokyo Chemical Industry) |
|---|---|
| CAS number | 831-61-8 |
| Molecular formula | — |
| Purity | — |
| Category | Analytical Chemistry > Analytical Standards > Analytical Standards (Others) |
| Pack sizes | available in the standard TCI catalogue pack sizes for this item; refer to the current TCI packaging options |
| Physical form | — |
| Storage | store according to the conditions stated on the manufacturer's label and certificate of analysis |
- Product code: E0854
- Intended use: analytical standard for the determination of total polyphenol content
Store the container tightly closed in a cool, dry, well-ventilated place, protected from direct light and away from heat sources, following the conditions printed on the manufacturer's label. Keep the material in its original container, or in an amber glass vial with a chemically resistant closure if aliquots are prepared, to limit exposure to air and moisture that could affect the phenolic groups. Handle in a fume hood or well-ventilated area, wearing safety glasses, gloves, and a laboratory coat, and avoid generating dust during weighing. Prepare stock solutions in water–ethanol or water–methanol mixtures shortly before use, label them clearly with the preparation date, and consult the manufacturer's safety data sheet before first use and for waste disposal.
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![Ethyl Gallate [for Determination of Total Polyphenol Content] (CAS 831-61-8) - TCI E0854 - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510E0854.jpg?v=1769142254&width=1445)