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Description
Guanidine Hydrochloride is the hydrochloride salt of guanidine and ranks among the most widely used chaotropic compounds in biochemistry laboratories, while also serving as an important material in perovskite solar cell research. In aqueous solution, the guanidinium cation is highly effective at disrupting the hydrogen bonding and hydrophobic interactions that stabilize protein structure. On the materials side, the guanidinium cation is larger than methylammonium, and for this reason it has been studied intensively as an additive and as a mixed cation for improving the stability of organic-inorganic perovskite thin films.
The property that makes this material a preferred choice is its very high solubility in water, which allows the preparation of highly concentrated solutions for protein denaturation, nucleic acid extraction, and stepwise protein refolding studies. As a denaturant, its strength is greater than that of urea at equivalent concentrations, so unfolding experiments can be carried out across a more compact concentration range. In the perovskite context, the addition of guanidinium has been reported to assist passivation of surface defects, extend charge carrier lifetime, and improve thin film morphology, making it a variable of interest in device optimization work.
In Indonesian laboratories, this reagent is typically encountered in two distinct settings. Biochemistry and molecular biology groups use it for routine protein and nucleic acid work, where concentrated stock solutions are prepared in advance and diluted to the working range required by each experiment. Materials science and renewable energy groups, meanwhile, use it as a cation source and additive in perovskite thin film formulations, where small and carefully controlled quantities are added to precursor solutions during device fabrication.
Laboratory Applications
- Protein denaturation studies, because the guanidinium cation disrupts hydrogen bonding and hydrophobic interactions that stabilize protein structure more strongly than urea at equivalent concentrations.
- Nucleic acid extraction, where the very high aqueous solubility of this salt allows preparation of the concentrated chaotropic solutions such protocols depend on.
- Protein refolding investigations, since concentrated stock solutions can be diluted stepwise to follow the unfolding and refolding transition across a controlled concentration series.
- Perovskite thin film additive work, as guanidinium incorporation has been reported to passivate surface defects and improve the morphology of organic-inorganic perovskite layers.
- Mixed cation perovskite formulation, because the guanidinium cation is larger than methylammonium and is studied for its effect on perovskite layer stability and charge carrier lifetime.
Specifications
- Brand: TCI
- CAS number: 50-01-1
- Category: Materials Science > Electronic Materials > Perovskite Solar Cell (PSC) Materials
- Catalogue number: G0162
- Pack sizes: available in the standard TCI catalogue pack sizes; please confirm the size required when ordering.
- Storage: store in a tightly closed container in a cool, dry place away from moisture.
Handling and Storage
Store this product in its original tightly closed container in a cool, dry, and well ventilated area. Because the material is highly soluble in water, it should be protected from moisture and humidity, which are common concerns in Indonesian laboratory conditions; keeping the container sealed between uses and avoiding prolonged exposure to open air will help maintain material quality. Handle with standard laboratory personal protective equipment, including gloves, safety glasses, and a laboratory coat, and weigh out quantities in a well ventilated area or fume hood to avoid inhalation of dust. Use clean, dry spatulas and glassware when dispensing, keep solutions clearly labelled with concentration and preparation date, and consult the manufacturer safety data sheet before first use.
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